Theranostics of skin neoplasms based on luminescence diagnostics in combination with photodynamic therapy in the absorption band of porphyrin

Abstract

We report the results of developing a technique for theranostics of skin neoplasms based on luminescence diagnostics in combination with photodynamic therapy (PDT) in the absorption band of porphyrin. It is shown that the therapeutic effect is achieved exclusively due to PDT, without the participation of the hyperthermia process, which occurs at temperatures above 42 °C. The Fluroscan gel [based on the dipotassium salt of the ytterbium complex of 2,4-di-(a-methoxyethyl)deuteroporphyrin IX (Yb-DMDP)] is used as a preparation for theranostics. The main photophysical properties and possible mechanisms of accumulation of nanosized low-toxic photosensitisers based on this compound are studied. It is shown that the Yb-DMDP compound in a DMSO solution (30% aqueous DMSO) enhances photophysical characteristics (luminescence lifetime 5-10 ms, luminescence quantum yield up to 1%, extinction coefficient ~1.96 × 105 M-1 cm-1 at a wavelength of 398 nm). Experimental animals are used to test the proposed technique for theranostics of tumours using the Fluroscan gel and a fibre-optic laser fluorimeter.