Therapecutic efficacy of the leaf extract of Croton joufra Roxb. against experimental cestodiasis in rats

Gogoi, Shyamalima; Yadav, Arun K.

doi:10.1007/s12639-016-0819-9

Cited by 5 publications

(7 citation statements)

References 22 publications

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“…To date, only a few studies on anthelmintic potentials of medicinal plants have backed their in vitro assays with in vivo studies. For example, Gogoi and Yadav (2016) and Gogoi and Yadav (2017) have supplemented their in vitro anthelmintic studies of plants with in vivo studies, using H. diminuta-rat and S. obvelata-mice models [18,30]. The findings are in agreement with that of Gogoi and Yadav (2016), who in their in vitro and in vivo efficacy testing of Caesalpinia bonducella, against H. diminuta and S. obvelata, also observed a dosedependent efficacy of extract [18].…”

Section: Discussionsupporting

confidence: 53%

“…In another study by Gogoi and Yadav (2017), the in vitro and in vivo anthelmintic efficacy of Croton joufra leaf extract was investigated against H. diminuta. The in vitro study showed a dose-dependent effect on parasite, and the highest concentration of the extract (30 mg/ml) caused mortality of in worms 1.53 ± 0.12 h, p<0.001 as compared to control value, b p<0.001 as compared to pre-treatment period, one-way ANOVA, followed by Tukey's test compared to 3.46 ± 0.10 h shown by PZQ (1 mg/ml).…”

Section: Discussionmentioning

confidence: 99%

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Studies on the anthelmintic potentials of the roots of Asparagus racemosus willd. (Asparagaceae)

Soren

Yadav

2021

Clin Phytosci

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Background The Santhal tribe in Assam, India use the roots of Asparagus racemosus (Asparagaceae) as a deworming remedy. The study aimed to investigate the anthelmintic credentials of this plant, using two representative groups of helminth parasites. Methods The in vitro testing was conducted against Hymenolepis diminuta (cestode) and Syphacia obvelata (nematode). Parasites were exposed to 10, 20 and 30 mg/ml concentrations of plant extract, and efficacy was adjudged on the basis of parasites paralysis and mortality. In vivo efficacy was examined using H. diminuta-rat and S. obvelata-mice models where animals were administered 125, 250 and 500 mg/kg doses of extract. Results In vitro assay, against H. diminuta revealed that at 30 mg/ml concentration the extract showed almost a comparable efficacy with that of reference drug praziquantel (PZQ) (1 mg/ml). The in vitro efficacy of extract against S. obvelata was however lower than H. diminuta. In vivo studies against H. diminuta at 500 mg/kg revealed 53.88 and 24 % reduction in eggs per gram (EPG) and worm counts respectively. Against S. obvelata the extract showed 26.61 and 30.93 % reduction for the same. Conclusions The findings of this study present suggest that the roots of A. racemosus are effective against intestinal helminthic infections and justifies its use as an anthelmintic in the traditional medicine of the Santhals.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Studies on the anthelmintic potentials of the roots of Asparagus racemosus willd. (Asparagaceae)

Soren

Yadav

2021

Clin Phytosci

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“…Protoberberines are another class of tetracyclic benzylisoquinolines that display antagonism at Sm.5HTR L . Rotundine is a constituent of traditional Chinese herbal medicine preparations, with activity against both cestodes (Das et al, 2009; Gogoi and Yadav, 2017) and schistosomes (Chan et al, 2016c). We previously identified racemic D/L-rotundine) as an antagonist at Sm.5HTR L and showed this compound inhibited movement of adult worms (Chan et al, 2016c).…”

Section: Resultsmentioning

confidence: 99%

“…The successful functional expression and miniaturization of a Sm.5HTR L profiling assay has enabled high-throughput compound screens to identify ligands with potential anti-schistosomal activity (Chan et al, 2018). Given these data validating Sm.5HTR L as a viable anti-schistosomal drug target, and the precedent of both tryptaminergic and benzylisoquinoline natural products as serotonergic ligands (Cabedo et al, 2009; Nichols, 2012; Harding, 2016) and antiparasitic compounds (Bonne, 1919; Iwasa et al, 1999; Nkwengoua et al, 2009; Das et al, 2009; Shahinas et al, 2012; Gogoi and Yadav, 2017), we performed a more detailed exploration of the properties of these pharmacophores that determine their efficacy as Sm.5HTR L ligands and activity against ex vivo cultured parasites.…”

Section: Discussionmentioning

confidence: 99%

Structure-activity profiling of alkaloid natural product pharmacophores against a Schistosoma serotonin receptor

Marchant

Harding

Chan

2018

International Journal for Parasitology: Drugs and Drug Resistan

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Serotonin (5-HT) is an important regulator of numerous aspects of flatworm biology, ranging from neuromuscular function to sexual maturation and egg laying. In the parasitic blood fluke Schistosoma mansoni, 5-HT targets several G-protein coupled receptors (GPCRs), one of which has been demonstrated to couple to cAMP and regulate parasite movement. This receptor, Sm.5HTRL, has been successfully co-expressed in mammalian cells alongside a luminescent cAMP-biosensor, enabling pharmacological profiling for candidate anti-schistosomal drugs. Here, we have utilized this assay to perform structure-activity investigations of 143 compounds containing previously identified alkaloid natural product pharmacophores (tryptamines, aporphines and protoberberines) shown to regulate Sm.5HTRL. These experiments mapped regions of the tryptamine pharmacophore amenable and intolerant to substitution, highlighting differences relative to orthologous mammalian 5-HT receptors. Potent Sm.5HTRL antagonists were identified, and the efficacy of these compounds were evaluated against live adult parasites cultured ex vivo. Such structure-activity profiling, characterizing the effect of various modifications to these core ring systems on Sm.5HTRL responses, provides greater understanding of pharmacophores selective for this target to aid future drug development efforts.

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“…Although the usage of traditional medicine has increased worldwide, research in this area is inadequate [6]. Several authors have scientifically validated the efficacy of plants used in traditional medicines to treat helminthiasis through both in vitro and in vivo studies and have found them to be efficacious [7,8].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro and in vivo anthelmintic efficacy of Cyperus compressus Linn., a traditionally used anthelmintic plant in parasite-animal models

Soren

Yadav

2020

Futur J Pharm Sci

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Background The decoction of the roots of Cyperus compressus (Cyperaceae) is used to treat helminth infection by the Santhal tribe of Assam. The study evaluated the anthelmintic efficacy claims of the plant C. compressus through pre-clinical in vitro and in vivo studies employing available parasite-animal models such as Hymenolepis diminuta-Wistar rat (cestode) and Syphacia obvelata-Swiss mice (nematode) models. Results Phytochemical analysis revealed the presence of alkaloids, glycosides, reducing sugars, flavonoids, terpenoids, tannins, and steroids. In vitro studies were conducted employing H. diminuta and S. obvelata. In vitro studies against H. diminuta revealed mortality of parasites at 8.3 ± 0.05 h at the highest concentration of C. compressus methanolic root extract (30 mg/ml), whereas reference drug praziquantel (PZQ), showed mortality at 5.84 ± 0.01 h. Against S. obvelata, in the same concentration of the extract, mortality of parasites occurred in a much later time of 24.13 ± 0.03 h, whereas in the reference drug albendazole (ABZ), the parasites showed mortality at 7.24 ± 0.08 h. In vivo studies against H. diminuta revealed 61.74% reduction in the eggs per gram (EPG) counts and 24% reduction in worm counts at the highest dose of 700 mg/kg body weight (b.w.) of plant extract. Against S. obvelata, at 700 mg/kg b.w., 28.92% and 33.85% reduction in EPG and worm counts were recorded respectively. Conclusion Although the reference drugs showed better in vitro and in vivo efficacy, the plant extract showed a better in vitro efficacy against cestode parasite compared to its nematode counterpart indicating that it possesses a better cestocidal efficacy. EPG reductions were higher against H. diminuta, whereas worm count reduction was higher against S. obvelata. The findings justify the use of C. compressus as an anthelmintic in the traditional medicine of the Santhals of India.

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Therapecutic efficacy of the leaf extract of Croton joufra Roxb. against experimental cestodiasis in rats

Cited by 5 publications

References 22 publications

Studies on the anthelmintic potentials of the roots of Asparagus racemosus willd. (Asparagaceae)

Studies on the anthelmintic potentials of the roots of Asparagus racemosus willd. (Asparagaceae)

Structure-activity profiling of alkaloid natural product pharmacophores against a Schistosoma serotonin receptor

Evaluation of in vitro and in vivo anthelmintic efficacy of Cyperus compressus Linn., a traditionally used anthelmintic plant in parasite-animal models

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