Therapeutic Activity of Pretazettine on Rauscher Leukemia: Combination of Antiviral Activity and Cellular Protein Inhibition

Abstract

Pretazettine hydrochloride (PTZ) has been found to inhibit protein synthesis, without being inhibitory to DNA and RNA, in Rauscher leukemic blood cells in mice for at least 6 h after its administration. With comparison to Virazole and cycloheximide, the specific anti-Rauscher virus activity of PTZ has been demonstrated only in acutely-infected NIH/3T3 cells but not in chronically-infected cells. It is not certain that the inhibitory action of PTZ on reverse transcriptase is contributory to its therapeutic activity in leukemic mice.

Discovery and Development of Therapeutic Drugs Against Lethal Human RNA Viruses: A Multidisciplinary Assault.

“…The molecular formula for axinfor halichondrin B and homohalichondrin B, respectively. astatin 1 (3) was deduced from high-field (400 MHz) 'H The characteristic mean graph "fingerprints" of the haliand ' 3 C NMR studies (see Table I of the supplementary chondrins were very similar; an analysis by the COMPARE material) in conjunction with the high-resolution FAB MS pattern-recognition algorithm 13 showed their mean graph peak matching experiments just noted. Combined 'H, 'H profiles to be most highly correlated to those produced by COSY, 'H, 13C COSY, 'H, 'H relayed COSY, 9" HMBC, 9b structurally unrelated, tubulin-binding standard agents" and NOESY experiments confirmed the amino acid sesuch as vincristine and taxol.…”

Effect of Pretazettine and Viva-Natural, a Dietary Seaweed Extract, on Spontaneous AKR Leukemia in Comparison with Standard Drugs

“…The mechanism of this rever sal effect of PTZ is not known. It may be due to the antiviral [2,3] or antitumor [4. 5] or the immunoenhancing action [personal commun.…”

“…a narcissus alkaloid, was therapeu tically active against Rauscher viral leukemia and spontaneous AKR T cell leukemia [1][2][3]. PTZ is in hibitory to the growth of murine retroviruses in cell cultures and in mice [2,3], PTZ is an inhibitor of viral reverse transcriptase [2] and is cytotoxic due to the specific inhibitory action on cellular protein synthesis [2], and therefore PTZ is also effective against nonviral transplantable tumors such as Ehrlich ascites and Lewis lung carcinoma [4.…”

“…a narcissus alkaloid, was therapeu tically active against Rauscher viral leukemia and spontaneous AKR T cell leukemia [1][2][3]. PTZ is in hibitory to the growth of murine retroviruses in cell cultures and in mice [2,3], PTZ is an inhibitor of viral reverse transcriptase [2] and is cytotoxic due to the specific inhibitory action on cellular protein synthesis [2], and therefore PTZ is also effective against nonviral transplantable tumors such as Ehrlich ascites and Lewis lung carcinoma [4. 5], Recently, we have found that an extract of a dietary seaweed Undaria pinnantifida, named Viva-Natural, was therapeutically active against Lewis lung carcinoma probably through the activation of immune systems [6], We have now studied the effect of PTZ and Viva-Natural for their antileukemic activity against AKR spontaneous T cell leukemia in comparison to standard cytotoxic and immunomodulating agents.…”

Therapeutic Activity of Pretazettine on Ehrlich Ascites Carcinoma: Adjuvant Effect on Standard Drugs in ABC Regimen

“…The proba ble active principle has been identified as pretazettine, an unstable alkaloid of the Amaryllidaceae family [5], and a method for the production of the stable hydrochloride of pretazettine (PTZ) for animal use has been established [5], In addition to the re markable antiviral activity against Rauscher and AKR leukemia [3,6], PTZ posesses a moderate or reversible cytotoxicity resulting from the specific inhibitory action on cellular protein synthesis [4,10]. Now we report the therapeutic activity of PTZ against Ehrlich ascites carcinoma and the beneficial adjuvant effect on the thera peutic activity of the individual standard an ticancer drugs in the ABC (adriamycin, BCNU, and cyclophosphamide) regimen on Ehrlich cells.…”