Therapeutic application of chick early amniotic fluid: effective rescue of acute myocardial ischemic injury by intravenous administration

Abstract

Myocardial regeneration has been considered a promising option for the treatment of adult myocardial injuries. Previously, a chick early amniotic fluid (ceAF) preparation was shown to contain growth-related factors that promoted embryonic growth and cellular proliferation, though the nature of the components within ceAF were not fully defined. Here we tested whether this ceAF preparation is similarly effective in the promotion of myocardial regeneration, which could provide an alternative therapeutic for intervening myocardial injury. In this study, a myocardial ischemic injury model was established in adult mice and pigs by multiple research entities, and we were able to show that ceAF can efficiently rescue damaged cardiac tissues and markedly improve cardiac function in both experimental models through intravenous administration. ceAF administration increased cell proliferation and improved angiogenesis, likely via down-regulation of Hippo-YAP signaling. Our data suggest that ceAF administration can effectively rescue ischemic heart injury, providing the key functional information for the further development of ceAF for use in attenuating myocardial injury.

Chick Early Amniotic Fluid (ceAF) Deters Tumorigenesis via Cell Cycle Arrest and Apoptosis

“…Furthermore, these fluids have low immunogenicity, anti-inflammation, and non-inflammatory features. Our previous studies also demonstrate the promising therapeutic potential of ceAF against cutaneous wound healing and myocardial infarctions [ 32 , 33 ]. Several in vivo experiments show that their tumor tropism has opened new horizons for cancer cell therapies [ 34 ].…”

Chick early amniotic fluid component improves heart function and protects against inflammation after myocardial infarction in mice

“…Our previous study indicated that ceAF can effectively rescue heart function after acute myocardial ischemic injury by intravenous administration in mice and swine ( 22 ). However, the active component was still unclear.…”

“…Preparation and separation of ceAF from 7-days chick embryo were performed as previously described ( 22 ). Briefly, the eggs impregnated with male seed were incubated at ∼38°C with 50% humidity for 7 days ( 23 ).…”

“…A more recent study showed that human AF (hAF) reduces C-reactive protein, a marker of inflammation, and improved clinical outcomes in hospitalized patients with COVID-19 after intravenous 10 ml hAF without obvious adverse events ( 21 ), indicating an immune-mediating role of hAF. Previously, we demonstrated that chick early amniotic fluid (ceAF) can effectively rescue heart dysfunction after ischemic injury in swine and mouse models ( 22 ). ceAF contains multiple fractions and the functional components of cardio-protective effect remain to be explored.…”

Editorial: New strategies to inhibit cell death in myocardial ischemia-reperfusion injury: how to succeed? Volume II

“…Cui et al describes the cardioprotective effects of the main active component, peak 8 (P8) fraction, from Chick early amniotic fluid (ceAF). In a previous study, the authors discovered that ceAF is very effective in the rescue of myocardial injury, in mice and pigs models of IR injury ( 1 ). In the present topic, they demonstrate that P8 treatment was able to decrease apoptosis in vitro in human embryonic stem cell-derived cardiomyocytes subjected to hypoxia and reoxygenation.…”