Therapeutic Applications for Adipose-Derived Stem Cells in Wound Healing and Tissue Engineering

McCarthy, Mary Ellen; Brown, Theodore; Bukowska, Joanna; Bunnell, Bruce A.; Frazier, Trivia; Wu, Xiying; Gimble, Jeffrey M.

doi:10.1007/s40778-018-0125-9

Cited by 13 publications

(9 citation statements)

References 92 publications

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“…In the current study, although IL-8 expression increased in Groups 1 and 2 compared to Group 4, there was no statistically significant difference (p> 0.05). As noted in the above studies (6,39,40), since inflammation was significantly high in non-diabetic wounds, target gene expressions were found to be quite low in all groups from the first days of the wound (days 4 and 8) to the last day (day 24).…”

Section: Discussionmentioning

confidence: 50%

“…Interleukin 8 (IL-8) gene is responsible for re-epithelization, tissue shaping, chemotaxis of neutrophil, proinflamation and the vessel development (9). The C-X-C Motif Chemokine Receptor 1 (CXCR1) gene is very important in the formation of angiogenesis with granulation tissue (6,10,11).…”

Section: öZmentioning

confidence: 99%

“…Wound healing process, cell migration to the wound area (macrophages, neutrophils, monocyte and mast cells) and triggering of the acute inflammatory response, as well as cell division and differentiation, are associated with the growth factors of protein and enzyme production and secretion of inflammatory cytokines. (6,7). Interleukin 6 (IL-6) gene is closely related to the differentiation and development of numerous dermal and epidermal cell types, and healing of skin wounds (8).…”

Section: öZmentioning

confidence: 99%

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Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits

Yavuz

Temamoğulları

Yiğin

et al. 2020

Dicle Üniversitesi Veteriner Fakültesi Dergisi

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Centaurium erythraea, a species of flowering plant in the Gentianaceae family, is a plant commonly used in the wound treatment. This study aims to investigate the effect of Centaurium erythraea for enhancing healing process in full-thickness skin wounds in diabetic rabbits. A total of 28 young female New Zealand rabbits were used and animals were divided into four equal groups randomly. Diabetes mellitus was created by injection of alloxan monohydrate. After 15 days following diabetes induction, two full-thickness wounds, a diameter of 2.6 cm, were created equidistantly from the dorsal median line under general anesthesia. For wounds, on a daily bas is, oily homogenized form of Centaurium erythraea was administered in Group 1 (n=7), while pomade form of the titrated extract of Centella asiatica in Group 2 (n=7), pure olive oil in Group 3 (n=7) and normal saline was applied in Group 4 (n=7). Wound edges were measured on the 4th, 8th, 12th, 16th, 20th and 24th days, furthermore, histopathological and genetic examinations were performed on tissue samples taken on the same days. Examining the wound diameters, healing rates in the Group 1 and Group 2 were found statistica lly significant compared to Group 3 and Group 4 (p<0,05). It was noted that the increase of connective tissue, collagen proliferation and epitelogenesis were significant in Groups 1 and 2 compared to Groups 3 and 4 (p<0.05). IL-6, IL-8 and CXCR1 gene expressions were observed to be low in all diabetic groups. Although the expression values were low in groups 1 and 2, it was found that there was a significant difference in the expression amounts of the target genes on the 4th and 8th days compared to other groups (p <0.0 5). When the wound healing was examined in terms of wound diameters, histopathology and gene expression, it occurred in Group 2, Group 1, Group 3 and Group 4, respectively. In conclusion, oily homogenized form of Centaurium erythraea was found to have a positive effect on full-thickness wound healing of diabetic rabbits.

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Section: Discussionmentioning

confidence: 50%

Section: öZmentioning

confidence: 99%

Section: öZmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits

Yavuz

Temamoğulları

Yiğin

et al. 2020

Dicle Üniversitesi Veteriner Fakültesi Dergisi

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“…A promising and costeffective source for MSCs is adipose tissue. Particularly, adipose-derived mesenchymal stem cells (ADSCs) represent significant cell type to use in this context for improving wound healing [20]. Extracting ADSCs from adipose tissue is less invasive than bone biopsies and the amount of extracted ADSCs per gram tissue is fivefold higher than what can be isolated from bone marrow [21].…”

Section: Introductionmentioning

confidence: 99%

Enhanced healing of a full-thickness wound by a thermoresponsive dressing utilized for simultaneous transfer and protection of adipose-derived mesenchymal stem cells sheet

Rezapour

Yeganeh

Gharibi

et al. 2020

J Mater Sci: Mater Med

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To boost the healing process in a full-thickness wound, a simple and efficient strategy based on adipose-derived mesenchymal stem cells (ADSCs) transplantation is described in this work. To increase the chance of ADSCs immobilization in the wound bed and prevent its migration, these cells are fully grown on the surface of a thermoresponsive dressing membrane under in vitro condition. Then, the cells sheet with their secreted extracellular matrix (ECM) is transferred to the damaged skin with the help of this dressing membrane. This membrane remains on wound bed and acts both as a cell sheet transfer vehicle, after external reduction of temperature, and protect wound during the healing process like a common wound dressing. The visual inspection of wounded skin (rat animal model) at selected time intervals shows a higher wound closure rate for ADSCs treated group. For this group of rats, the better quality of reconstructed tissue is approved by results of histological and immunohistochemical analysis since the higher length of the new epidermis, the higher thickness of re-epithelialization layer, a higher level of neovascularization and capillary density, and the least collagen deposition are detected in the healed tissue.

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“…Adipose-derived stem cells (ADSCs) are one kind of mesenchymal stem cells (MSCs) that has been most widely used in the clinic in recent years. ADSCs have been transplanted in patients to treat a variety of diseases, such as knee osteoarthritis, Crohn's disease, diabetes mellitus type 1, and chronic obstructive pulmonary disease [1][2][3][4] … In recent years, off-theshelf ADSCs have been developed to treat diseases like ischemic heart disease/ heart failure 5 , Crohn's disease 6 … Therefore, understanding optimal long-term in vitro expansion of ADSCs is important to yield adequate numbers of stem cells for laboratory and clinical applications. However, one major issue related to in vitro expansion of ADSCs is the aging of the cells during the expansion period.…”

Section: Introductionmentioning

confidence: 99%

High glucose induces early senescence in adipose-derived stem cells by accelerating p16 and mTOR

Pham

2019

Biomed. Res. Ther.

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Introduction: The senescence of stem cells is the primary reason that causes aging of stem cell-containing tissues. Some hypotheses have suggested that high glucose concentration in diabetic patients is the main factor that causes senescence of cells in those patients. This study aimed to evaluate the effects of high glucose concentrations on the senescence of adipose-derived stem cells (ADSCs). Methods: ADSCs were isolated and expanded from human adipose tissues. They were characterized and confirmed as mesenchymal stem cells (MSCs) by expression of surface markers, their shape, and in vitro differentiation potential. They were then cultured in 3 different media- that contained 17.5 mM, 35 mM, or 55 mM of D-glucose. The senescent status of ADSCs was recorded by the expression of the enzyme beta-galactosidase, cell proliferation, and doubling time. Real-time RT-PCR was used to evaluate the expression of p16, p21, p53 and mTOR. Results: The results showed that high glucose concentrations (35 mM and 55 mM) in the culture medium induced senescence of human ADSCs. The ADSCs could progress to the senescent status quicker than those cultured in the lower glucose-containing medium (17.5 mM). The senescent state was related to the up-regulation of p16 and mTOR genes. Conclusion: These results suggest that high glucose in culture medium can trigger the expression of p16 and mTOR genes which cause early senescence in ADSCs. Therefore, ADSCs should be cultured in low glucose culture medium, or normal glucose concentration, to extend their life in vitro as well as in vivo.

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Therapeutic Applications for Adipose-Derived Stem Cells in Wound Healing and Tissue Engineering

Cited by 13 publications

References 92 publications

Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits

Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits

Enhanced healing of a full-thickness wound by a thermoresponsive dressing utilized for simultaneous transfer and protection of adipose-derived mesenchymal stem cells sheet

High glucose induces early senescence in adipose-derived stem cells by accelerating p16 and mTOR

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