Therapeutic effect of adipose-derived mesenchymal stem cells on Cisplatin induced testicular damage in adult male albino rat

Meligy, Fatma Y.; Elgheed, Amal T Abo; Alghareeb, Shymaa M

doi:10.1080/01913123.2019.1572256

Cited by 44 publications

(40 citation statements)

References 75 publications

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“…Treatment with resveratrol in Groups II and IV started 48 h before starting the experiment and treatment in all Groups continued for further 4 successive weeks. The doses of resveratrol [21,22] and cisplatin [23][24][25] were selected as per previously published. Protein content were specified using protein assay kit.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

Cisplatin induced testicular damage through mitochondria mediated apoptosis, inflammation and oxidative stress in rats: impact of resveratrol

Aly

Eid

2020

Endocr J

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The target of this study was to explore the role of mitochondria mediated apoptosis and inflammation in cisplatininduced testicular damage and to evaluate the ameliorative effect of resveratrol. Adult male Wistar rats were randomly allocated to 4 groups. Group I (Control) received normal saline, Group II (Resveratrol) received resveratrol (50 mg/kg/day), Group III (Cisplatin) received cisplatin (7.5 mg/kg/week, i.p.) and Group IV (Resveratrol + Cisplatin) received resveratrol and cisplatin in the same regimen of treatment. Treatment with resveratrol in Groups II and IV started 48h before cisplatin injection and continued for further 4 successive weeks. Cisplatin-treated rats showed reduced body weight, absolute testes weight and sperm count, motility and viability. On the other hand, cisplatin treatment increased the percentage of sperm abnormalities. It also decreased serum testosterone level, mitochondrial membrane potential while, increased cytochrome C liberation from the mitochondria into the cytosol. The activities of caspase-3 &-9 were increased. The level of TNF-α, IL-6 and Bax were increased whereas Bcl-2 was decreased. Oxidative stress markers were found to increase with a concomitant reduction in the antioxidant enzymes and GSH levels. These results were confirmed by immunohistochemical and histopathological analysis. Contrary to all these results, there were improvements in cisplatin induced testicular damage through attenuation of mitochondria mediated apoptosis, inflammation, and oxidative stress owing to resveratrol pretreatment. Thus, resveratrol, as a potential therapeutic agent, may hold promise in preventing mitochondria mediated apoptosis and inflammation in cisplatin-induced testicular damage in rats.

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Section: Animals and Treatmentsmentioning

confidence: 99%

Cisplatin induced testicular damage through mitochondria mediated apoptosis, inflammation and oxidative stress in rats: impact of resveratrol

Aly

Eid

2020

Endocr J

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“…22 In this study, the human ADSC dosage used was 1x10 6 cells in 1000µL PBL solution into the rete testis (about 1x10 6 cells (1 cc)) via intratesticular route. 23 The administration of intratesticular human ADSC is based on the presence of confounding blood-testis-barrier (BTB), which could complicate the action of ADSC if given as an intravascular injection. This finding is also supported by Shinohara et al in 2018, which proved that direct stem cell intratesticular injection into the seminiferous tubule of an infertile rat is a significant route of administration.…”

Section: Discussionmentioning

confidence: 99%

Intratesticular Human Adipose-Derived Stem Cell (hADSC) Transplantation Decreased Oxidative Stress in Testicular Torsion Model of Wistar Rat

Siregar¹,

Noegroho²,

Adriansjah³

et al. 2021

RRU

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“…Until now, MSCs isolated from various tissues have been used in cell-based therapies to promote the repair of testicular damage or treatment of male infertility. Although the results of these studies have been promising, further research in this area is needed [24,34,35]. In this regard, the use of cells possessing immunosuppressive properties, which occur naturally in affected testicular tissue, could be bene cial.…”

Section: Discussionmentioning

confidence: 99%

Sertoli Cells Possess Immunomodulatory Properties and the Ability of Mitochondrial Transfer Similar to Mesenchymal Stem Cells

Porubská

Vasek

Somova

et al. 2021

Preprint

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It is becoming increasingly evident that selecting an optimal source of mesenchymal stem cells (MSCs) is crucial for the successful outcome of MSC-based therapies. During the search for cells with potent regenerative properties, Sertoli cells (SCs) have been proven to modulate immune response in both in vitro and in vivo models. Based on morphological properties and expression of surface markers, it has been suggested that SCs could be a kind of MSCs, however, this hypothesis has not been fully confirmed. Therefore, we compared several parameters of MSCs and SCs, with the aim to evaluate the therapeutic potential of SCs in regenerative medicine. We showed that SCs successfully underwent osteogenic, chondrogenic and adipogenic differentiation and determined the expression profile of canonical MSC markers on the SC surface. Besides, SCs rescued T helper (Th) cells from undergoing apoptosis, promoted the anti-inflammatory phenotype of these cells, but did not regulate Th cell proliferation. MSCs impaired the Th17-mediated response; on the other hand, SCs suppressed the inflammatory polarisation in general. SCs induced M2 macrophage polarisation more effectively than MSCs. For the first time, we demonstrated here the ability of SCs to transfer mitochondria to immune cells. Our results indicate that SCs are a type of MSCs and modulate the reactivity of the immune system. Therefore, we suggest that SCs are promising candidates for application in regenerative medicine due to their anti-inflammatory and protective effects, especially in the therapies for diseases associated with testicular tissue inflammation.

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Therapeutic effect of adipose-derived mesenchymal stem cells on Cisplatin induced testicular damage in adult male albino rat

Cited by 44 publications

References 75 publications

Cisplatin induced testicular damage through mitochondria mediated apoptosis, inflammation and oxidative stress in rats: impact of resveratrol

Cisplatin induced testicular damage through mitochondria mediated apoptosis, inflammation and oxidative stress in rats: impact of resveratrol

Intratesticular Human Adipose-Derived Stem Cell (hADSC) Transplantation Decreased Oxidative Stress in Testicular Torsion Model of Wistar Rat

Sertoli Cells Possess Immunomodulatory Properties and the Ability of Mitochondrial Transfer Similar to Mesenchymal Stem Cells

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