Therapeutic effect of caffeic acid phenethyl ester oncerulein-induced acute pancreatitis

Abstract

AIM:To evaluate the therapeutic role of caffeic acid phenethyl ester (CAPE) in a rat model of ceruleaninduced acute pancreatitis (AP). METHODS:Seventy male Wistar albino rats were divided into seven groups. Acute edematous pancreatitis was induced by subcutaneous cerulein injection (20 μg/kg) four times at 1-h intervals. CAPE (30 mg/kg) was given by subcutaneous injection at the beginning (CAPE 1 group) and 12 h after the last cerulein injection (CAPE 2 group). Serum amylase, lipase, white blood cell count, and tumor necrosis factor (TNF)-α levels were measured, and pancreatic histopathology was assessed. RESULTS:In the AP group, amylase and lipase levels were found to be elevated and the histopathological evaluation showed massive edema and inflammation of the pancreas, with less fatty necrosis when compared with sham and control groups. Amylase and lipase levels and edema formation decreased significantly in the CAPE therapy groups (P < 0001); especially in the CAPE 2 group, edema was improved nearly completely (P = 0001). Inflammation and fatty necrosis were partially recovered by CAPE treatment. The pathological results and amylase level in the placebo groups were similar to those in the AP group. White blood cell count and TNF-α concentration was nearly the same in the CAPE and placebo groups.

The effect of quercetin on cerulein-induced acute pancreatitis

“…4,21 Pro-inflammatory cytokines such as TNF-α and IL-6 released by damaged pancreas cells play an important role in the pathogenesis of AP. 3,31 There are still no specific therapies for AP. Treatment remains substantially supportive for symptoms.…”

“…At present, all attention is focused on antioxidant and anti-inflammatory therapies. 2,3,4,6,18,20 Quercetin has been reported to exert multiple biological effects such as antioxidant, anti--inflammatory, and antihistaminic effects etc.…”

“…After auto-digestion, a massive infiltration of neutrophils and macrophages lead to a local and systemic inflammatory response. 3,5 This is partially caused by the release of cytokines from acinar cells. Reactive oxygen substances (ROS) may also contribute to the damage of pancreatic acinar cells.…”

Protective Effects of Lycopene on Cerulein-Induced Experimental Acute Pancreatitis in Rats

“…to that in humans and occurs within a short time [27]. This model is used widely to study potential agents for the treatment of AP [28]. Cerulein, an analogue of CCK acting through the CCK receptors, yields exaggerated stimulation of acinar cells, which leads to prematuration of trypsinogen, followed by lysosomal degradation of intracellular organelles within autophagic vacuoles in acinar cells and marked interstitial edema [29].…”

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

“…Flavonoids, phenolic acids like caffeic acid phenethyl ester (CAPE), and esters are the most biologically active compounds (Table 2) (Burdock, 1998;Daugsch et al, 2008;Baumann et al, 1980;Silva et al, 2007). These compounds exert multiple effects on bacteria, fungi and viruses and also present anti-inflammatory, antioxidant, immunomodulatory, wound healing, antiproliferative and antitumor activities (Machado et al, 2008;Pagliarone et al, 2009;Buyukberber et al, 2009;Jaganathan & Mandal, 2009;Medic-Saric et al, 2009;Pillai et al, 2010;Moreira et al, 2011;Lotfy, 2006).…”