Therapeutic effect of co-culture of rat bone marrow mesenchymal stem cells and degenerated nucleus pulposus cells on intervertebral disc degeneration

Shi, Ming; Zhao, Yan; Sun, Yue; Xin, Daqi; Xu, Wei-long; Zhou, Boyuan

doi:10.1016/j.spinee.2021.05.007

Cited by 26 publications

(20 citation statements)

References 22 publications

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“…Furthermore, BMSC-Exos were able to prevent and mitigate NPC apoptosis induced by acidic pH by repressing caspase-3 expression and attenuating caspase-3 cleavage. When NPCs are placed in the pathological state of low blood supply, BMSC-Exos could increase miR-155 expression in NPCs, thereby downregulating Bach1 expression and upregulating heme oxygenase-1(HO-1) expression, activating autophagy in NPCs, inhibiting the level of apoptosis, thereby inhibiting IDD (Shi et al, 2021). Besides, BMSC-Exos could reduce the apoptosis rate of NPCs induced by tumor necrosis factor-α (TNF-α), while the miR-532-5p level was decreased in apoptotic NPCs.…”

Section: Inhibiting Nucleus Pulposus Cell Apoptosis and Promoting Nuc...mentioning

confidence: 99%

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Liang

Han

Hai

et al. 2022

Front. Cell Dev. Biol.

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Exosomes are extracellular vesicles formed by various donor cells that regulate gene expression and cellular function in recipient cells. Exosomes derived from mesenchymal stem cells (MSC-Exos) perform the regulatory function of stem cells by transporting proteins, nucleic acids, and lipids. Intervertebral disc degeneration (IDD) is one of the main causes of low back pain, and it is characterized by a decreased number of nucleus pulposus cells, extracellular matrix decomposition, aging of the annulus fibrosus, and cartilage endplate calcification. Besides, nutrient transport and structural repair of intervertebral discs depend on bone and cartilage and are closely related to the state of the bone. Trauma, disease and aging can all cause bone injury. However, there is a lack of effective drugs against IDD and bone injury. Recent MSC-Exos fine tuning has led to significant progress in the IDD treatment and bone repair and regeneration. In this review, we looked at the uniqueness of MSC-Exos, and the potential treatment mechanisms of MSC-Exos with respect to IDD, bone defects and injuries.

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Section: Inhibiting Nucleus Pulposus Cell Apoptosis and Promoting Nuc...mentioning

confidence: 99%

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Liang

Han

Hai

et al. 2022

Front. Cell Dev. Biol.

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“…The second reference ( n = 92) titled “Differentiation of mesenchymal stem cells transplanted to a rabbit degenerative disc model - Potential and limitations for stem cell therapy in disc regeneration” was published by Sakai D on Spine in 2005 [ 35 ], The third reference ( n = 92) was “Transplantation of mesenchymal stem cells embedded in Atelocollagen((R)) gel to the intervertebral disc: a potential therapeutic model for disc degeneration”, published by Sakai D on Biomaterials in 2003 ( Figure 4(a) ). On the other hand, the most co-citation journal was Spine ( n = 3179) [ 36 ], followed by European Spine Journal ( n = 774) [ 37 ] and Spine Journal ( n = 582) [ 38 ] ( Figure 4(b) ). Furthermore, the most co-citation author was Sakai D ( n = 547) [ 9 ], the second was Risbud MV ( n = 333) [ 34 ], and the third was Richardson SM ( n = 234) [ 39 ] ( Figure 4(c) ).…”

Section: Resultsmentioning

confidence: 99%

Researches on Stem and Progenitor Cells in Intervertebral Discs: An Analysis of the Scientific Landscape

Cheng

Yang

Hai

et al. 2022

Stem Cells International

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Low back pain (LBP) is a common clinical symptom, and the prevalence is ranged from 60% to 70%. With the deepening of basic research, the development of intervertebral disc regeneration-oriented cell therapy, especially stem and progenitor cells therapy, showed good research prospects and was expected to become new methods of treatment for LBP. Our study is aimed at analyzing the scientific output of stem and progenitor cells in intervertebral discs and at driving future research into new publications. Researches focused on this file were searched from the Science Citation Index Expanded (SCI-E) of the Web of Science (WOS) core collection database and were screened according to inclusion criteria. We evaluated and visualized the results, including annual publications, citations, authors, organizations, countries, research directions, funds, and journals by bibliometric website, VOSviewer, and Citespace softwares on May 27, 2022. A total of 450 original articles and reviews were included, and the overall trend of the number of publications rapidly increased. In worldwide, China and the USA were the leading countries for research production. The retrieved 450 publications received 14322 citations, with an average of 31.83 citations and an H-index of 62. The most high-yield author, organization, country, research directions, funds, and journals were Chen QX from Zhejiang University, Zhejiang University, China, Cell Biology, National Natural Science Foundation of China, and Spine, respectively. Keywords cluster analysis showed the research hotspots in the future, including “human intervertebral disc”, “adipose-derived mesenchymal stem cell”, “intervertebral disc degeneration”, “degenerative disc model”, “nucleus pulposus regeneration”, “human cartilage”, “3d culture”, “shrinkage-free preparation”, and “polylactide disc”. Furthermore, with accumulating evidence demonstrating the role of stem and progenitor cells in intervertebral discs, “microenvironment”, “activation”, “intervertebral disc degeneration”, and “oxidative stress” are becoming the research frontiers and trends.

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“…The levels of apoptotic proteins such as caspase-3 and caspase-9 increased after H 2 O 2 treatment but the expression of these proteins was downregulated by BMSC EV treatment [88]. BMSC EVs increase the level of autophagy in NPCs and decrease apoptosis level by upregulating the miR-155 expression, which targets Bach1 in NPCs and promotes expression level of HO-1 thus abrogating IVDD [100].…”

Section: Antiapoptotic and Regenerative Activities Of Msc Evsmentioning

confidence: 94%

Mesenchymal Stem Cell-Derived Extracellular Vesicles: Immunomodulatory Effects and Potential Applications in Intervertebral Disc Degeneration

Xing

Zhang

et al. 2022

Stem Cells International

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Intervertebral disc (IVD) degenerative disease is a common health problem worldwide. Administration of mesenchymal stem cells (MSCs) in intervertebral disc degeneration (IVDD) has been widely explored in recent years. However, transplantation of MSCs is restricted by several factors. Currently, paracrine signaling is one of the main mechanisms by which MSCs play a therapeutic role in disc regeneration. Extracellular vehicles (EVs) are the main paracrine products of MSCs. They show great potential as an effective alternative to MSCs and play immunomodulation roles such as anti-inflammatory effects, antioxidative stress, antiapoptosis, and antiextracellular matrix (ECM) degradation during treatment of IVDD. This review focuses on the immunomodulatory effect of MSC EVs and their potential applications.

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Therapeutic effect of co-culture of rat bone marrow mesenchymal stem cells and degenerated nucleus pulposus cells on intervertebral disc degeneration

Cited by 26 publications

References 22 publications

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Researches on Stem and Progenitor Cells in Intervertebral Discs: An Analysis of the Scientific Landscape

Mesenchymal Stem Cell-Derived Extracellular Vesicles: Immunomodulatory Effects and Potential Applications in Intervertebral Disc Degeneration

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