Therapeutic Effect of Curcumin on Scanning Electron Microscopy of Rat Adrenal Gland in Experimental Fluorosis

Abstract

Aim: The present study was designed to investigate the therapeutic role of Curcumin against fluoride induced toxicity on adrenal gland of rats by using scanning electron microscopy (SEM).
 Methodology: Wistar albino rats were divided randomly into six groups The group I was administered with 1 ml of deionized water/kg b.w./day orally for 40 days. The Groups II and III were given 300 and 600 mg of NaF/kg b.w./day for the same period, respectively. The group IV was given 200 mg/kg b.w. of Curcumin for 20 days. The Groups V and VI were treated with 300 and 600 mg of NaF/kg b.w./day for 40 days respectively were post-treated with 200 mg of Curcumin for next 20 days. The animals were excised and adrenal tissue was taken out and processed for SEM.
 Results: The results revealed that rats exposed to 300 mg/kg b.w./day of NaF showed rough edges, numerous microvilli and damaged surface with crystal depositions. Also, numerous granules were distributed all over the surface. The rats treated with 600 mg/kg b.w./day of NaF showed decellularized adrenal tissue along with network of collagen fibres. Moreover, adrenal gland surface displayed abrasions and distorted cuboidal cells. The filopodia were prominent on the surface and wall of cavity possessed rough outline. After post-treatment with Curcumin, fluoridated adrenal gland of rats showed normal structure, reappearance of cuboidal cells on the surface as well as less number of microvilli and filopodia.
 Conclusion: The post-treatment with Curcumin possess therapeutic potential against NaF induced toxicity in adrenal gland of rats.

Histopatholgical Alteration in the Spleen of Male Albino Rats Exposed to Sodium Fluoride with the Protective Role of Resveratrol

“…As regarding the sections of control group stained with Masson Trichrome indicated that collagen fibers showed normal arrangement in the capsule and in the extending trabaculae. On the other side the mean percent area of collagen fibers in the NaF treated group showed significant increase in comparison to control group and these finding proved by morphometric analysis and these results were in line with Fayza et al,(2014) who said that there was significant increase in collagen fibers percent area in rat lungs treated with NaF and also these results were in agreement with Shashi1 and Manisha., (2021) who showed that adrenal gland of NaF treated rat showed that adrenal tissue was decellularized with increased collagen deposition. The induction of collagen synthesis was due to increased secretion of growth factors and liberation of cytokines by leukocytes (Castejón and Arismendi., 2006).…”