Therapeutic effect of ginsenoside Rd in rats with TNBS-induced recurrent ulcerative colitis

Abstract

Ulcerative colitis (UC) is characterized by oxidative and nitrosative stress and neutrophil infiltration. In the present study, we aimed to investigate the therapeutic effect of ginsenoside Rd (GRd) in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced recurrent UC. After UC was twice-induced by intracolonic injection of TNBS, rats were intragastrically administered different doses of GRd per day for 7 days. The colonic lesions and inflammation were evaluated both histologically and biochemically. Compared with the TNBS group, GRd treatment facilitated recovery of pathologic changes in the colon after induction of recurrent UC, as evidenced by a significant reduction of colonic weight/length ratio and macroscopic and microscopic damage scores (p < 0.01). The myeloperoxidase and inducible nitric oxide synthase activities with malonyldialdehyde and nitric oxide levels in colonic tissues were significantly decreased in the GRd group compared with those in the TNBS group (p < 0.01). GRd treatment was associated with remarkably increased superoxide dismutase and glutathione peroxidase activities. Results showed a valuable effect of GRd against TNBS-induced recurrent UC by inhibiting neutrophil infiltration and promoting the antioxidant capacity of the damaged colonic tissue.

Anti-inflammatory effects of Brazilian ginseng (Pfaffia paniculata) on TNBS-induced intestinal inflammation: Experimental evidence

“…The phytochemical analysis performed in our study showed that the P. paniculata extract contained a series of compounds belonging to these chemical classes. These substances have been found to be responsible for a number of biological activities, such as analgesic and anti-inflammatory properties [31,[59][60][61][62], as well as antitumour effects [28,[63][64][65][66]. Importantly, none of the phytochemical analyses showed the presence of β-ecdysterone in the studied extract (Fig.…”

“…This reduction in MPO activity may be related to a decrease in inflammatory cell infiltration as a consequence of the anti-inflammatory effects of the compounds contained in the P. paniculata extract. These compounds are chemically similar to the ginsenosides, which are substances with demonstrated antiinflammatory actions according to studies by [61,62,[75][76][77].…”

Ginsenosides Regulate PXR/NF-κB Signaling and Attenuate Dextran Sulfate Sodium–Induced Colitis

“…Although the anti-inflammatory effects of ginsenosides and its remedy effects against IBD have been well reported, the molecular mechanisms remain established (Yang et al, 2012a;Li et al, 2014;Ye et al, 2014). Here we hypothesized that ginsenosides may elicit its anti-inflammatory effects in IBD via restoring PXR/NF-kB signaling in the inflamed colon.…”

A Review on Chemical-Induced Inflammatory Bowel Disease Models in Rodents

“…Study conducted by Yang et al stated that recurrent ulcerative colitis model can also be induced in Wistar rats by instilling TNBS (100 mg/kg) into the colon through an obtuse cannula. This is followed by a second instillation with TNBS at a dose of 37.5 mg/kg into the colon 14 days after the first induction of colitis leading to generation of recurrent model of ulcerative colitis [48]. …”

“…Five to eight days after presensitization, mice are rechallenged by intrarectal administration of 70~150 µl of 0.75~1% oxazolone in 45-50% ethanol solution which leading to induction of colitis. Animals are sacrificed after 7~12 days after presensitization to assess severity of colitis [24,43,48,82]. Huang et al reported that colitis is induced in male BALB/C mice by presensitizing with transdermal application of 200 µl of a 3% (w/v) oxazolone solution in acetone/olive oil (4:1) on the shaven skin.…”