Therapeutic effect of local photothermal heating of gold nanoparticle-coated self-expandable metallic stents for suppressing granulation tissue formation in the mouse colon

Abstract

Purpose To investigate the therapeutic effect of local photothermal (PT) heating on suppression of stent-induced granulation tissue formation in mouse colon. Materials and methods A gold nanoparticle (GNP)-coated self-expandable metallic stent (SEMS) was prepared using a two-step synthesis process for local PT heating under near-infrared laser irradiation. Twenty-four mice were randomly divided into two groups of 12 and subjected to SEMS placement in the colon. Group A received a GNP-coated SEMS without local heating and Group B received a GNP-coated SEMS and underwent local heating at 55°C after SEMS placement. The therapeutic effect of local heating was assessed by comparing the histopathological, immunohistochemical, and endoscopic results. Results Four mice were excluded because of stent migration (n = 3, group B) or death (n = 1, group A). Stent-induced granulation tissue-related variables were significantly lower in group B than in group A (p < 0.001). In vivo endoscopic images, 4 weeks after stent placement, showed granulation tissue formation over the wire mesh in group A and relatively good patency of the stented colon with no definite irregularities in group B. There was more vascular endothelial growth factor (VEGF) positivity in group A than in group B. Conclusion Local PT heating suppresses granulation tissue formation after stent placement in mouse colon.

Prevention Effect of TGF-β Type I Receptor Kinase Inhibitor in Esophageal Stricture Formation after Corrosive Burn

“…There is no established medical treatment during the acute stage that can prevent further stricture formation. Experimentally, many treatment options have been used to reduce stricture formation [3][4][5][6][7][8][9]23]; however, only a few of them are used clinically.…”

“…Collagen synthesis is enhanced by TGF-β1. It plays an important role in the transition from inflammatory phase to proliferative phase, particularly in fibroblasts and collagen deposition, indicating the pathophysiological pathway of stenosis formation [23].…”

Prevention Effect of TGF-β Type I Receptor Kinase Inhibitor in Esophageal Stricture Formation after Corrosive Burn

“…There is no established medical treatment during the acute stage that can prevent further stricture formation. Experimentally, many treatment options have been used to reduce stricture formation [3][4][5][6][7][8][9]22]; however, only a few of them are used clinically.…”

“…Collagen synthesis is enhanced by TGF-β1. It plays an important role in the transition from inflammatory phase to proliferative phase, particularly in fibroblasts and collagen deposition, indicating the pathophysiological pathway of stenosis formation [22].…”

3D-printed versatile biliary stents with nanoengineered surface for anti-hyperplasia and antibiofilm formation

“…The slides were stained with H&E and MT. Histological evaluation using H&E determined the degree of submucosal inflammatory cell infiltration, thickness of submucosal fibrosis by eight points around circumference average, and percentage of granulation tissue formation of ductal cross-sectional area stenosis was calculated as follows [ [57] , [58] , [59] ]: …”