Therapeutic Effect of Nigella sativa on Alcohol-induced Liver Disease in Rats

Mosbah, Ahmed; Zettal, H; Khither, Hanane; Mosbah, Camelia; Chaouche, Noreddine Kacem; Benboubetra, Mustapha

doi:10.9734/ejmp/2017/34661

Cited by 2 publications

(2 citation statements)

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“…Liver toxicity induced with ethanol diet consumption (Lieber-DeCarli Liquide diet) was assessed in terms of (i) state of oxidative stress markers (GSH, CAT, SOD, LPO), (ii) biochemical markers and lipid profile (iii) histopathology of liver tissue (25)(26)(27)(28)(29)(30). Results showed an association between oxidative stress and liver injury caused by alcohol consumption.…”

Section: Alcohol Liver Toxicitymentioning

confidence: 99%

Nigella sativa L. oil: Study of its toxicity, antiradical activity, and effect on circulating xanthine oxidoreductase

Asma

Khither

Camélia

et al. 2022

J Herbmed Pharmacol

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Introduction: Nigella sativa L. is a widely used medicinal plant throughout the world. The low toxic effects and low price of this plant make it an excellent treatment choice for many diseases. The present study aims to investigate the hepatoprotective effect of N. sativa L. total oil (TO) and its neutral lipid fraction (NLF) via the estimation of circulating xanthine oxidoreductase (XOR) level and anti-XOR antibodies titer. Methods: Antiradical activities of TO and NLF, in vitro, were carried out using three reactive oxygen species (ROS), superoxide anion, hydroxyl radical, and hydrogen peroxide. In vivo study was conducted to determine the possible protective effects of TO and NLF against ethanol-induced hepatotoxicity in rats feeding Lieber-DeCarli liquid diet in both sera and liver homogenate. Before conducting the hepatoprotective effects, we assessed the toxicity of our extracts using the same animal model. The administrated doses of 400 mg/kg for TO and 300 mg/kg for NLF showed no toxic effects. Results: ELISA assay indicated a significant increase (P<0.001) in the level of XOR and the titer of anti-XOR antibodies in rats treated with ethanol compared to the control group. After treatment with TO and NLF, the titer of anti-XOR antibodies and the level of XOR decreased significantly compared to the control group. Conclusion: XOR plays an important role in alcohol liver pathologies as a major source of free radicals. In addition, TO and NLF have significant potential as liver protective agents and might be utilized as new antioxidant therapeutics.

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Section: Alcohol Liver Toxicitymentioning

confidence: 99%

Nigella sativa L. oil: Study of its toxicity, antiradical activity, and effect on circulating xanthine oxidoreductase

Asma

Khither

Camélia

et al. 2022

J Herbmed Pharmacol

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“…Antioxidants have a major role in scavenging of free radicals, which are abundant in cell. For example, [20][21][22][23]. Due to these effects, N.sativa seed oil was chosen to prepare nanoformulations in our study.…”

Section: Introductionmentioning

confidence: 99%

Preparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cells

et al. 2021

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To improve bioavailability and stability of hydrophobic and hydrophilic compounds, nanoemulsions are good alternatives as delivery systems because of their nontoxic and non-irritant nature. Glutathione (GSH) suffers from low stability in water, where its encapsulation in nanoemulsions is a powerful strategy to its stability in aqueous systems. The aim of this study was to obtain nanoemulsions from the hydrophobic/hydrophilic contents of N. sativa seed oil so as to improve GSH stability along with bioavailability of N. sativa seed oil. Then, the prepared nanoemulsions were tested for in vitro hepatoprotective activity against ethanol toxicity. To the best of our knowledge, there is no study on the test of nanoemulsions by the combination of Nigella sativa seed oils and GSH in hepatoprotective activity. Here, nanoemulsions with different contents were prepared using Nigella sativa seed oils. Content analyses and characterization studies of prepared nanoemulsions were carried out. In order to investigate the protective effects against to ethanol exposure, THLE-2 cells were pretreated with nanoemulsions for two hours with the maximum benign dose (0.5 mg/mL 3 of nanoemulsions). Ethanol (400 mM) was introduced to pre-treated cells and nontreated cells for 48-or 72-hour periods, followed by cell viability assay was carried out. Fluorescence microscopy tests revealed the introduction of the nanoemulsions into THLE-2 cells. The findings show that nano-formulations have promising in vitro hepatoprotective effects on the THLE-2 cell line against ethanol exposure.

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Therapeutic Effect of Nigella sativa on Alcohol-induced Liver Disease in Rats

Cited by 2 publications

References 0 publications

Nigella sativa L. oil: Study of its toxicity, antiradical activity, and effect on circulating xanthine oxidoreductase

Nigella sativa L. oil: Study of its toxicity, antiradical activity, and effect on circulating xanthine oxidoreductase

Preparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cells

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