Therapeutic effects and mechanism of Atractylodis rhizoma in acute lung injury: Investigation based on an Integrated approach

Shi, Kun; Wang, Yan; Xiao, Yangxin; Tu, Jian; Zhou, Zhongshi; Cao, Guosheng; Liu, Yanju

doi:10.3389/fphar.2023.1181951

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…DC. (https://mpns.science.kew.org/ (accessed on 7 June 2022)) [11]. This compound possesses several beneficial effects such as anti-inflammatory, antioxidant, and anti-viral properties, as well as gastrointestinal regulation and neuroprotection [12,13].…”

Section: Introductionmentioning

confidence: 99%

Atractylenolide III Ameliorates Bile Duct Ligation-Induced Liver Fibrosis by Inhibiting the PI3K/AKT Pathway and Regulating Glutamine Metabolism

Wang

Shi

et al. 2023

Molecules

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Liver fibrosis is one of the leading causes of hepatic sclerosis and hepatocellular carcinoma worldwide. However, the complex pathophysiological mechanisms of liver fibrosis are unknown, and no specific drugs are available to treat liver fibrosis. Atractylenolide III (ATL III) is a natural compound isolated from the plant Atractylodes lancea (Thunb.) DC. that possesses antioxidant properties and the ability to inhibit inflammatory responses. In this study, cholestatic hepatic fibrosis was induced in mice using a bile duct ligation (BDL) model and treated with 10 mg/kg and 50 mg/kg of ATL III via gavage for 14 days. ATL III significantly reduced the liver index, lowered serum ALT and AST levels, and reduced liver injury in bile-duct-ligated mice. In addition, ATL III significantly attenuated histopathological changes and reduced collagen deposition. ATL III reduced the expression of fibrosis-related genes α-smooth muscle actin (α-SMA), Collagen I (col1a1), Collagen IV (col4a2), and fibrosis-related proteins α-SMA and col1a1 in liver tissue. Using RNA sequencing (RNA-seq) to screen molecular targets and pathways, ATL III was found to affect the PI3K/AKT singling pathway by inhibiting the phosphorylation of PI3K and AKT, thereby ameliorating BDL-induced liver fibrosis. Gas chromatography–mass spectrometry (GC-MS) was used to evaluate the effect of ATL III on liver metabolites in BDL mice. ATL III further affected glutamine metabolism by down-regulating the activity of glutamine (GLS1) and glutamine metabolism. ATL III further affected glutamine metabolism by down-regulating the activity of glutaminase (GLS1), as well as glutamine metabolism. Therefore, we conclude that ATL III attenuates liver fibrosis by inhibiting the PI3K/AKT pathway and glutamine metabolism, suggesting that ATL III is a potential drug candidate for treating liver fibrosis.

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Section: Introductionmentioning

confidence: 99%

Atractylenolide III Ameliorates Bile Duct Ligation-Induced Liver Fibrosis by Inhibiting the PI3K/AKT Pathway and Regulating Glutamine Metabolism

Wang

Shi

et al. 2023

Molecules

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Medicinal Herbal Extracts: Therapeutic Potential in Acute Lung Injury

Lee,

Yun

et al. 2024

Future Pharmacology

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Acute lung injury (ALI) is induced by pneumonia, sepsis and other conditions. The disease characteristics include severe lung inflammation, in which various cells, such as epithelial cells, macrophages, and neutrophils, play a pivotal role. Corticosteroids and antibiotics are used to treat ALI; however, they may have side effects. Cumulative data confirm that traditional herbal medicines exert therapeutic effects against endotoxin-induced inflammatory responses in both in vitro and in vivo ALI studies. This review briefly describes the anti-ALI effects of medicinal herbal extracts (MHEs) and their molecular mechanisms, especially focusing on Toll-like receptor 4/nuclear factor kappa B cell pathways, with a brief summary of in vitro and in vivo ALI experimental models. Thus, the present review highlights the excellent potential of MHEs for ALI therapy and prevention and may also be useful for the establishment of in vitro and in vivo ALI models.

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Bojungikgi-tang Alleviates Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages and C57BL/6 Mice

Kim

Han

Park

2023

PIM

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Background: This study evaluated the anti-inflammatory effect of Bojungikgi-tang (BJT) in a model of acute lung injury (ALI) in mice.Methods: Murine cell line macrophages (RAW264.7) were treated with BJT for 30 minutes before lipopolysaccharide (LPS) treatment. The levels of cytokines, mRNAs, proteins, and related markers were investigated. In addition, BJT (200 mg/kg/day) was administered orally to C57BL/6 mice for 2 weeks prior to an intraperitoneal injection with LPS to induce sepsis and ALI; 24 hours post LPS injection, mice were sacrificed and blood was collected from the infraorbital vein. Lung tissue was harvested, hematoxylin and eosin staining was performed, the wet/dry ratio of the lung tissue was measured, and the serum cytokine levels were analyzed.Results: Compared with LPS treatment, BJT suppressed LPS-induced mRNA expression and secretion of inflammatory cytokines in RAW264.7 macrophages. Furthermore, inducible nitric oxide synthase, cyclooxygenase-2, toll-like receptor 4, phosphorylation of mitogenactivated protein kinases, and phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells were inhibited by BJT. In mice, LPS-induced pathological changes in lung tissues, such as abnormal histological structures, immune cell infiltration, and lung edema were less severe following BJT treatment. BJT inhibited the LPS-induced increase of cytokines such as interleukin 4, 6, 10, and tumor necrosis factor alpha.Conclusion: BJT had an inhibitory effect in the pathological progress of LPS-induced sepsis and ALI and may be a promising therapeutic agent in the future.

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Therapeutic effects and mechanism of Atractylodis rhizoma in acute lung injury: Investigation based on an Integrated approach

Cited by 3 publications

References 44 publications

Atractylenolide III Ameliorates Bile Duct Ligation-Induced Liver Fibrosis by Inhibiting the PI3K/AKT Pathway and Regulating Glutamine Metabolism

Atractylenolide III Ameliorates Bile Duct Ligation-Induced Liver Fibrosis by Inhibiting the PI3K/AKT Pathway and Regulating Glutamine Metabolism

Medicinal Herbal Extracts: Therapeutic Potential in Acute Lung Injury

Bojungikgi-tang Alleviates Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages and C57BL/6 Mice

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