Therapeutic Effects of Hydrogen-Rich Solution on Aplastic Anemia in Vivo

Abstract

Background: Aplasitc anemia (AA) is a bone marrow failure syndrome characterized by an immune-mediated destruction of hematopoietic stem cells. Though clinical symptoms could be ameliorated by bone marrow transplantation and/or immunosuppressive therapy, frequent recurrence and especially evolution of clonal hematologic diseases remains problematic clinically. Cytokines such as interferon-γ (INF-γ), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) secreted by autologous T cells are closely related with the development of AA. Hydrogen-rich solution was reported to inhibit the levels of cytokines including INF-γ, TNF-α and IL-6 in vivo in recent studies. This study was to investigate the potential therapeutic effects of hydrogen-rich solution on AA in vivo. Methods: AA model was determined in vivo by mice and body weights of the mice were used as the basic physiological index. Peripheral blood cells were calculated to evaluate the hematologic recovery degree. Bone marrow nucleated cells (BMNCs), tissue histology, as well as CFU-S and CFU-GM forming units were used to evaluate the recovery of bone marrow microenvironment. The ratio of CD4⁺ and CD8⁺ cells were examined along with cytokine levels in serum to determine the efficacy of H₂-rich solution on the affected immunological functions. Results: Body weight and number of peripheral blood cells were significantly improved for mice in the H₂-rich solution treated groups as compared with those with AA. The number of BMNCs and CFUs increased markedly and the bone marrow microenvironment was also improved significantly. The experimental group restrained the cell apoptosis, relieved hyperemia and accelerated tissue repair. The number of CD4⁺ and CD8⁺ cells as well as the ratio of CD4/CD8 increased to normal gradually, while the levels of TNF-α, IFN-γ, and IL-6 in serum decreased after H₂-rich solution treatment. Conclusion: Our study firstly showed that hydrogen-rich solution accelerated the recovery of either hematological or immunological recovery on aplastic anemia mice. This finding suggests hydrogen-rich solution as a potential clinical therapeutic agent for AA.

Radioprotective Effect of Grape Seed Proanthocyanidins In Vitro and In Vivo

“…Caudal vein WBC was counted in cell count plate at the same time of the day as described previously [ 7 ].…”

Molecular hydrogen: a preventive and therapeutic medical gas for various diseases

“…Combination therapy with H 2 and hyperoxia or HS treatment provides enhanced therapeutic efficacy via both anti-oxidative and anti-inflammatory mechanisms, and might be a clinically feasible approach to treat sepsis [ 228 – 231 ]. Other studies indicated that H 2 administration accelerated recovery in aplastic anemia mice [ 232 ], increased blood alkalinity in physically active men [ 233 , 234 ], inhibited collagen-induced platelet aggregation in healthy humans and rats [ 235 ], and elevated serum anti-oxidative function in thoroughbred horses [ 236 ].…”

TNF-α-induced programmed cell death in the pathogenesis of acquired aplastic anemia

“…TNF-a, IFN-g and IL-6 in serum decreased after the application of H2-rich solution [96]. Zhai et al [97] reported that brucine can reduce the levels of TNF-a and IFN-g through inhibiting the proliferation of T lymphocytes in AA patients.…”