Therapeutic effects of peptide P140 in a mouse periodontitis model

Akiyama, Kentaro; Aung, Kyaw Thu; Talamini, Laura; Huck, Olivier; Kuboki, Takuo; Muller, Sylviane

doi:10.1007/s00018-022-04537-2

Cited by 5 publications

(1 citation statement)

References 39 publications

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“…The software accompanying the micro-CT machine was used to reconstruct the scanned images and further produce 3D microarchitectures. As described previously, 25 the area of remaining bone volume was determined between the mesial and distal root of the first molar according to the region of interest (ROI) in micro-CT 2D sagittal sections. In addition, the alveolar bone loss was determined by the area of the lingual root surfaces of the first mandibular molar as well as the distance from cemento-enamel junction to alveolar bone crest on the mesial, middle and distal regions of the first mandibular molar.…”

Section: Methodsmentioning

confidence: 99%

Interactions of Fibroblast Subtypes Influence Osteoclastogenesis and Alveolar Bone Destruction in Periodontitis

Wang¹,

Wang²,

Yang³

et al. 2023

JIR

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Background To analyze the fibroblasts subtypes in the gingival tissues of healthy controls, gingivitis and periodontitis patients, as well as the effects of interaction between subtypes on alveolar bone destruction. Methods Gingival tissues were divided into three groups according to clinical and radiographic examination, and the immunostaining of EDA+FN was assessed. Fibroblasts from gingiva developed colony formation units (CFUs) and induced Trap+MNCs. The expression of osteoclastogenesis-related genes was assessed by real-time PCR. Variances in the gene profiles of CFUs were identified by principal component analysis, and cluster analysis divided CFUs into subtypes. The induction of Trap+MNCs and gene expression were compared among individual or cocultured subtypes. The fibroblast subtypes exerted critical effect on Trap+MNCs formation were selected and edited by CRISPR/Cas to investigate the influence on osteoclastogenesis in the periodontitis in mice. Results Most periodontitis samples exhibited intensive EDA+FN staining (P < 0.05), and these fibroblasts also induced most Trap+MNCs among three groups; consistently, fibroblasts from periodontitis highly expressed genes facilitating osteoclastogenesis. According to gene profiles and osteoclastogenic induction, four clusters of CFUs were identified. The proportion of clusters was significantly different (P < 0.05) among three groups, and their interaction influenced osteoclastogenic induction. Although Cluster 4 induced less osteoclasts, it enhanced the effects of Clusters 1 and 3 on Trap+MNCs formation (P < 0.05). EDA knockout in Cluster 4 abrogated this promotion (P < 0.05), and decreased osteoclasts and alveolar bone destruction in experimental periodontitis (P < 0.05). Conclusion Heterogeneous fibroblast subtypes affect the switch or development of periodontitis. A subtype (Cluster 4) played important role during alveolar bone destruction, by regulating other subtypes via EDA+FN paracrine.

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Section: Methodsmentioning

confidence: 99%