Therapeutic effects of the total lignans from Vitex negundo seeds on collagen-induced arthritis in rats

Jiang, Rui; Ban, Yanfei; Xu, Wei-Heng; Ni, Hua; Guo, Yaoli; Geng, Yiya; Zang, Yali; Zheng, Cheng‐Jian

doi:10.1016/j.phymed.2019.152825

Cited by 47 publications

(47 citation statements)

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“…Human breast cancer cells MCF-7 and MDA-MB-231 were grown in 6-wells plates up to 60% confluence, and treated with BD at indicated doses for 72 h. The cells were washed 2 times with phosphate buffered saline (PBS), lysed in 40 μL of cold RIPA lysis buffer containing 1X Phenylmethylsulfonyl Fluoride (PMSF) and then used for western blot assay. Western blot assay was performed as described previously ( 22 ). Membranes were probed using rabbit antibodies specific for ZFP36, EGR1, FOS, and GAPDH (1:1000), followed by incubation with HRP-conjugated secondary antibody (1:2000).…”

Section: Methodsmentioning

confidence: 99%

Network-Based Approach to Identify the Antiproliferative Mechanisms of Bruceine D in Breast Cancer From the Cancer Genome Atlas

2020

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Bruceine D (BD) is a natural compound extracted from a Chinese herb Brucea javanica that has been used for anti-inflammatory and anti-cancer treatment. However, little is reported about BD's effects in breast cancer tumorigenesis. In this paper, we aimed to investigate the effect of BD in breast cancer and elucidate the potential mechanism of BD by integrated multiple databases. Our data suggested BD inhibited MCF-7 and MDA-MB-231 cells proliferation and promoted cells apoptosis. We integrated multiple bioinformatics analysis strategies to identify genes, hub modules and pathways associated with BD treatment. Three key pathways, including AMIT_SERUM_RESPONSE_40_MCF10A, BILD_HRAS_ONCOGENIC_SIGNATURE, and NAGASHIMA_NRG1_SIGNALING_UP were identified to be associated with therapeutic effects of BD in breast cancer. Additionally, we validated the key genes by using quantitative real-time PCR and western blot. In conclusion, these findings revealed potential molecular mechanisms of BD to treat breast cancer by affecting AMIT_SERUM_RESPONSE_40_MCF10A, BILD_HRAS_ONCOGENIC_SIGNATURE, and NAGASHIMA_NRG1_SIGNALING_UP pathways and regulating expression of ZFP36, EGR1, and FOS.

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Section: Methodsmentioning

confidence: 99%

Network-Based Approach to Identify the Antiproliferative Mechanisms of Bruceine D in Breast Cancer From the Cancer Genome Atlas

2020

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“…are the major proin ammatory cytokines which modulate the pathologic RA progress [22][23][24] . COX-2 (PTGS2) is the rate-limiting enzyme of arachidonic acid metabolism, which controls the production of PGE 2 , the proin ammatory mediators of autoimmunity activities [25][26][27] .…”

Section: Network Pharmacology Unraveled Crucial Anti-ra Targets Of Geramentioning

confidence: 99%

The Anti-RA Activities of a Couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata, Explored by Untargeted Metabolomics and Network Pharmacology.

Yang¹,

Shen

et al. 2020

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Background The couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata (GERA), is an established formula extensively used in Chinese medicine for treating rheumatoid arthritis (RA). However, the anti-RA mechanisms of GERA are still unclear. This paper aims to explore the anti-RA mechanisms of GERA by a combined strategy of untargeted metabolomics and network pharmacology. Methods Three water extracts were prepared as propotions: Gastrodia Elata compared with Radix Aconitic Lateralis (w/w): 1:1, 3:2, or 2:3. The untargeted metabolomics were executed with UPLC-MS. The metabolites were annotated and identified by Human metabolome database (HMDB) and Lipid maps database. Finally, key genes and pathways related to anti-RA activities of GERA were mined by network pharmacology. Results The untargeted metabolomics profile displayed that four differentially expressed metabolites were involved in isoflavonoid biosynthesis and biosynthesis of unsaturated fatty acids (p < 0.05). Among these differential metabolites, the essential ingredients of GERA were linoleic acid, daidzein, and daidzin. The principal targets of anti-RA activities of GERA were IL-6, TNF, VEGFA, TP53, CASP3 and PTGS2. Thirty anti-RA targets of GERA were majorly belonged to pathways response for anti-inflammation, endothelial function and apoptosis, suggesting the fundamental process of RA treatment. Conclusion The anti-RA activities of GERA were based on the inhibition of inflammation and regulation of endothelial function and apoptosis.

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“…Rheumatoid arthritis (RA) is a chronic, systematic and autoimmune inflammatory disease that results in progressive synovitis, joint swelling and damage, synovial hyperplasia, and bone and cartilage erosion (Yousefi et al, 2014;Saleem et al, 2020;Zhu et al, 2020). Although the etiology of RA is intricate and vague, inflammatory factors, including pro-inflammatory cytokines, anti-inflammatory cytokines and inflammatory mediators, are responsible for bone and cartilage erosions, and play a crucial role in this disease (Wang et al, 2017a;Rui et al, 2019;Saleem et al, 2020). Additionally, the serum levels of these inflammatory mediators were determined by enzyme-linked immunosorbent assay (ELISA) kits (Lin et al, 2013;Wang et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the serum levels of these inflammatory mediators were determined by enzyme-linked immunosorbent assay (ELISA) kits (Lin et al, 2013;Wang et al, 2017a). Currently, immunosuppressants, biological agents and disease-modifying anti-rheumatic drugs (DMARDs), and steroidal and non-steroidal anti-inflammatory drugs (NSAIDs) are commonly used for the treatment of RA, but most of them display long-term adverse effects, toxicity and comorbidities (Dai et al, 2020;Li et al, 2019a, Li et al, 2019bRui et al, 2019). As a result of this, exploring effective and safe anti-RA drug candidates from natural products, especially traditional folk medicines, could be a momentous breakthrough.…”

Section: Introductionmentioning

confidence: 99%

“…Traditional Chinese medicines (TCMs) are decisive complementary and alternative medicines, which have been verified to be effective treating RA for centuries with more safety and little side-effects in China and other Southeast Asian countries (Bao et al, 2018;Li et al, 2019a, Li et al, 2019bLin et al, 2013;Jing et al, 2019;Yang et al, 2020). Pterospermum heterophyllum Hance is native only to China and widely distributed in Fujian, Guangdong, Guangxi and Hainan provinces, belonging to the Sterculiaceae family (Editorial Committee of Traditional Chinese Medicine 1999; Yang et al, , 2019a.…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

et al. 2020

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Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease without effective and beneficial drugs. Many traditional folk medicines have been proven to be effective in treating RA. Among these, the root of Pterospermum heterophyllum Hance has been widely used as a traditional remedy against RA in China, but there is no scientific basis yet. The aim of this study was to investigate for the first time the chemical compositions and therapeutic effect of P. heterophyllum on adjuvant-induced arthritis (AIA) model in rats. 73 compounds were identified from P. heterophyllum based on ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-qTOF-MS/MS), and flavonoids may be partly responsible for the major anti-arthritic effect. In parallel, the P. heterophyllum extract at 160, 320, and 640 mg/kg/day were orally administered to rats for 22 days after post-administration adjuvant. The results showed that P. heterophyllum remarkably ameliorated histological lesions of the knee joint, increased body weight growth, decreased arthritis score, reduced thymus and spleen indices in model rats. Moreover, P. heterophyllum treatment persuasively downregulated the levels of rheumatoid factor (RF), C-reactive protein (CRP), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-17, cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX) and matrix metalloproteinase-2 (MMP-2), and observably upregulated IL-4 and IL-10 levels in model rats. These findings suggest that P. heterophyllum has a prominent anti-RA effect on AIA rats by modulating the inflammatory responses, and supports the traditional folk use of this plant.

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Therapeutic effects of the total lignans from Vitex negundo seeds on collagen-induced arthritis in rats

Cited by 47 publications

References 50 publications

Network-Based Approach to Identify the Antiproliferative Mechanisms of Bruceine D in Breast Cancer From the Cancer Genome Atlas

Network-Based Approach to Identify the Antiproliferative Mechanisms of Bruceine D in Breast Cancer From the Cancer Genome Atlas

The Anti-RA Activities of a Couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata, Explored by Untargeted Metabolomics and Network Pharmacology.

Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

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