Therapeutic Efficacy of Malachite Green-Based Photodynamic Therapy in Acute Myeloid Leukemia

Çalışkan, Serçin Özlem; DURAN>, Ömer Furkan; Aslan, Cem; Özen, Hüsne; Caliskan, Metin; Sağkan, Rahşan İlikçi

doi:10.16899/jcm.1251398

Cited by 3 publications

(1 citation statement)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is the subject of clinical research both as a treatment and as a complementary therapeutic procedure to other treatments (13). Although previous studies have shown that acute monocytic leukemia and chronic myeloid leukemia cells can be effectively killed using a variety of classical photosensitizers such as zinc phthalocyanine, Nile blue, 5-aminolevulinic acid (5-ALA), hypericin, malachite green, and Photolon ® (17)(18)(19)(20)(21)(22), the use of PDT It has received relatively less interest in the treatment of leukemia. The results of our study showed that malachite green-mediated PDT caused a significant decrease in the proliferation of HL-60 cells compared to both controls and malachite green.…”

Section: Discussionmentioning

confidence: 99%

Anti-Leukemic Effect of Malachite Green-Mediated Photodynamic Therapy by Inducing ER Stress in HL-60 Cells

ÇALIŞKAN,

BAYRAK,

ÖZLEM ÇALIŞKAN

2024

Medical Records

Self Cite

View full text Add to dashboard Cite

Aim: In this study, we aimed to examine of anti-leukemic effect and the relationship with endoplasmic reticulum (ER) stress of malachite green-mediated photodynamic therapy (PDT) in HL60 acute myeloid leukemia cells. Material and Methods: The cells were incubated with different concentrations (3.125, 1.56, 0.39, 0.195, 0.0975, 0. 04875 μM) of malachite green for one hour, were exposed to delivered an irradiance of 0.47 mW/cm2 and a fluence of 0.84 J/cm2 at 30 minutes. Also, HL60 cells were exposed for PDT with light only and both in the presence or absence of malachite green. MTT was used to evaluate cell viability and immunocytochemical staining was used to determine ER stress markers Glucose-regulated protein 78 (GRP78) and Protein Kinase R-like ER Kinase (PERK). Results: Cell viability was significantly decreased in the treatment group (combination of malachite green and light) compared to the malachite, light and control groups. Moreover, immunocytochemical staining scoring showed that GRP78 and PERK were significantly upregulated in the treatment group compared to other groups. Conclusion: The results presented in this study indicate that ER stress may contribute to the cytotoxicity occurring in HL60 cancer cells after malachite green-mediated PDT. Future studies will be crucial in shedding light on the molecular mechanisms underlying ER stress that may occur after PDT. These findings lay the foundation for further investigations in this area.

show abstract

Section: Discussionmentioning

confidence: 99%

Anti-Leukemic Effect of Malachite Green-Mediated Photodynamic Therapy by Inducing ER Stress in HL-60 Cells

ÇALIŞKAN,

BAYRAK,

ÖZLEM ÇALIŞKAN

2024

Medical Records

Self Cite

View full text Add to dashboard Cite

show abstract

Monitoring Apoptosis and Myeloid Differentiation of Acridine Orange‐Mediated Sonodynamic Therapy‐Induced Human Promyelocytic Leukemia HL60 Cells

Caliskan,

Ilikci‐Sagkan,

Bayrak

et al. 2024

J of Ultrasound Medicine

View full text Add to dashboard Cite

ObjectivesIn the treatment of acute myeloid leukemia (AML), conventional therapies can lead to severe side effects and drug resistance. There is a need for alternative treatments that do not cause treatment resistance and have minimal or no side effects. Sonodynamic therapy (SDT), due to its noninvasive, multiple repeatability, localized treatment feature and do not cause treatment resistance, emerges as an alternative treatment option. However, it has not received sufficient attention in the treatment of AML especially acute promyelocytic leukemia (APL). The aim of the study was to investigate the potential differentiation and antileukemic effects of acridine orange (AO)‐mediated SDT on HL60 cells.MethodsCell viability was determined by the 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) method in the control, ultrasound, AO concentrations, and ultrasound‐exposed AO concentrations groups. Transmission electron microscopy (TEM) was used to determine morphology, and flow cytometry was used to determine apoptosis, DNA cycle, cell volume, mitochondria membrane potential (Δψm), reactive oxygen species (ROS) production, and differentiation markers (CD11b and CD15) expressions. Additionally, toluidine blue staining for semithin sections was used to determine differentiation.ResultsThe cytotoxicity of AO‐mediated SDT on HL60 cells was significantly higher than other groups, and TEM images showed that it caused various morphological changes typical for apoptosis. Flow cytometry results showed the presence of early apoptosis, subG1 arrest, loss of Δψm, increase of intracellular ROS production, decreased cell volume, and increased expression of CD11b (1.3‐fold) antigen and CD15 (1.2‐fold) antigen.ConclusionData showed that AO‐mediated SDT significantly induced apoptosis in HL60 cells. Increased expression of CD11b and CD15 antigens and morphological findings demonstrated that AO‐mediated SDT contributes to granulocytic differentiation in HL60 cells. AO‐mediated SDT has potential as an alternative treatment of APL.

show abstract

Investigation of Endoplasmic Reticulum Stress and Apoptosis Caused by Malachite Green-Mediated Sonodynamic Therapy in HL60 Cells

ÇALIŞKAN,

BAYRAK,

ÖZLEM ÇALIŞKAN

2024

Medical Records

Self Cite

View full text Add to dashboard Cite

Aim: Sonodynamic antitumor therapy is a promising, novel method for the treatment of cancer. To determine the effects of malachite green (MG) in the presence of ultrasound (US), MG was tested in vitro on HL60 cells at different concentrations as a sonodynamic compound. We investigated cell viability, morphology, and the occurrence of ER stress after MG-mediated sonodynamic therapy (SDT) in HL60 cells. Materials and Methods: Four groups were formed, including a control group, a group subjected to ultrasound (US) only, a group treated with various concentrations of MG, and a group treated with US using the same concentrations. The cells were treated with 1MHz ultrasound at 2 W/cm2 for 3 minutes. The assessment of cell viability was conducted 24 hours post-treatment through the utilization of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cell morphology and apoptotic index were determined using Giemsa staining, while GRP78 and PERK expressions were determined through immunocytochemistry staining. Results: The cell cytotoxicity of HL60 cells significantly increased after MG-mediated sonodynamic therapy. After treatment, apoptotic cells with micronuclei were observed morphologically. Significant levels of GRP78 and PERK expression were observed in all groups, except for PERK expression in the US group, compared to the control group. Conclusion: The induction of ER stress, accompanied by intense apoptosis and a marked decrease in cell viability, demonstrates the potential of MG-mediated sonodynamic therapy in cancer treatment. Investigating ER stress as a molecular target may contribute to improving the treatment method.

show abstract

Therapeutic Efficacy of Malachite Green-Based Photodynamic Therapy in Acute Myeloid Leukemia

Cited by 3 publications

References 37 publications

Anti-Leukemic Effect of Malachite Green-Mediated Photodynamic Therapy by Inducing ER Stress in HL-60 Cells

Anti-Leukemic Effect of Malachite Green-Mediated Photodynamic Therapy by Inducing ER Stress in HL-60 Cells

Monitoring Apoptosis and Myeloid Differentiation of Acridine Orange‐Mediated Sonodynamic Therapy‐Induced Human Promyelocytic Leukemia HL60 Cells

Investigation of Endoplasmic Reticulum Stress and Apoptosis Caused by Malachite Green-Mediated Sonodynamic Therapy in HL60 Cells

Contact Info

Product

Resources

About