Therapeutic evaluation of acupoint stimulation with needle-scapelon on rat model of degenerative cervical intervertebral discs

Sun, Qinran; Liu, Fangming; Gao, Min; Wu, Wenqing; Liu, Weiju; Yin, Cong; Zhu, Zhiling; Zhang, Lijin; Ding, Xin; Guo, Yanping; Gong, Hui; Sun, Xin; Zhang, Daoping

doi:10.1016/j.biopha.2018.11.070

Cited by 3 publications

(6 citation statements)

References 15 publications

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“…The induction method is relatively mature and highly reproducible and is widely used in CS research. Our research group also verified the accuracy and feasibility of this model in previous studies [7] . Compared with the control group, the Miyamoto score of the CS group decreased significantly, apoptosis increased, and the mechanical pain threshold decreased significantly.…”

Section: Discussionsupporting

confidence: 78%

“…Twenty-four rats were randomly selected to construct models of CS according to the reference and preliminary study [7] . After 1 week of adaptive feeding, 24 rats were randomly selected to be anesthetized by intraperitoneal injection of 3% pentobarbitone sodium at a dosage of 30 mg/kg.…”

Section: Cs Modelsmentioning

confidence: 99%

“…Finally, morphological changes were observed under a microscope. An intervertebral disc was evaluated using the grading standard as previously reported [7] . Morphological changes were observed under a microscope.…”

Section: Sleep Deprivationmentioning

confidence: 99%

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Establishment and Evaluation of Rat Models of Cervical Spondylopathy with Yin Deficiency Syndrome

Yang

Liu

Sun

et al. 2023

Preprint

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Objective: Cervical spondylosis (CS) with Yin deficiency syndrome is an important classification of traditional Chinese medicine (TCM) symptoms of CS. However, there is no animal model for studying this TCM syndrome. This study aimes to establish and evaluate rat models of cervical spondylopathy with Yin deficiency syndrome. Method: Thirty-six Sprague–Dawley male rats were randomly divided into the blank control (control), CS and CS with Yin deficiency syndrome (YCS) groups (n = 12 rats/group). CS was induced using cervical static–dynamic imbalance to mimic disk degeneration (except in the control group). After 30 days of the CS rat model, rats in the YCS group were subjected to sustained sleep deprivation for 168h. After different induction times, the rats in each group were observed for behavior and weight. Pain behavior was assessed by a withdrawal response to von Frey filament application, and heart rate and blood pressure were measured using a rat noninvasive sphygmomanometer. Intervertebral disc pathology sections were observed using hematoxylin and eosin staining and an electron microscope. Western blotting was used to evaluate the protein level of collagen-II, Bcl-2, Bax and Bcl-2/Bax expression in the cervical intervertebral disc. Determination of related laboratory serum indexes, cyclic adenosine monophosphate, and cyclic guanosine phosphate were conducted using enzyme-linked immunosorbent assay. Results: The laboratory indexes in the YCS group were significantly different from those in the control and CS groups (P < 0.05), and indicators of 72-, 120-, and 178-h sleep deprivation showed varying degrees of difference from those of the CS group. Conclusion. After establishing a model of CS, continuous sleep deprivation for 72 h was used to create a rat model of CS with Yin deficiency syndrome. The established rat models of CS with Yin deficiency syndrome met the clinical and Chinese medicine characteristics, and thus, they can be expected to become an ideal model for studying CS with Yin deficiency syndrome in the future.

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Section: Discussionsupporting

confidence: 78%

Section: Cs Modelsmentioning

confidence: 99%

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Establishment and Evaluation of Rat Models of Cervical Spondylopathy with Yin Deficiency Syndrome

Yang

Liu

Sun

et al. 2023

Preprint

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“…e clinical manifestations of CSM vary according to the location and degree of spinal cord compression, representing abnormal limb function, unstable walking, and even quadriplegia. CSM has a high disability rate, which not only brings great pain to patients but also brings a certain degree of the medical economic burden to society and families [9,10]. e primary cause [11,12].…”

Section: Discussionmentioning

confidence: 99%

Relationship between Severity of Disease and Postoperative Neurological Recovery in Patients with Cervical Spondylotic Myelopathy Combined with Developmental Spinal Stenosis

Wang

Zhang

Peng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. The study aimed to investigate the correlation between the severity of disease and postoperative neurological recovery in patients with cervical spondylotic myelopathy (CSM) combined with developmental spinal stenosis. Methods. A retrospective analysis of the clinical data of 114 CSM patients combined with developmental spinal stenosis admitted to our hospital from June 2019 to June 2020 was performed. All of the patients who underwent posterior cervical unidoor vertebroplasty were divided into the mild, moderate, and severe groups according to the Torg–Pavlov ratio. The clinical data including patients’ age, course of spinal cord high signal change, and first onset age were collected. The recovery time, preoperative, and postoperative Japanese Orthopaedic Association (JOA) scores of patients in each group were compared with the calculation of the improvement rate. The correlation between the severity of disease and postoperative neurological recovery in CSM patients combined with developmental spinal stenosis was analyzed by Pearson correlation. The factors influencing postoperative neurological recovery were analyzed using multivariate logistic regression analysis. The receiver operating characteristic curve (AUC) was used to evaluate the value of each influencing factor in predicting postoperative recovery. Results. Significant differences were observed in the proportion of linear hyperintensity changes in the spinal cord, the age of first onset, the course of the disease, and the Torg–Pavlov ratio among the mild, moderate, and severe groups ( P < 0 . 05 ). The postoperative recovery time of the moderate and severe groups was significantly higher than that of the mild group, while the preoperative JOA score was significantly lower than that of the mild group. On the other hand, the postoperative recovery time of the severe group was prominently higher than that of the moderate group, whereas the preoperative JOA score was observably lower than that of the moderate group ( P < 0 . 05 ). Pearson correlation analysis showed that the postoperative recovery time was significantly negatively correlated with the Torg–Pavlov ratio, age at first onset, and disease course (r = −0.359, −0.502, −0.368, P < 0 . 05 ), while it was positively correlated with spinal cord linear high-signal changes (r = 0.641, P < 0 . 05 ). Multifactorial logistic regression analysis revealed that the Torg–Pavlov ratio, age at first onset, and disease course were protective factors, while spinal cord linear high-signal alterations were risk factors affecting the recovery time of postoperative neurological function ( P < 0 . 05 ). The area under the curve (AUC) of the Torg–Pavlov ratio, linear hyperintensity changes in the spinal cord, age at first onset, and disease duration in predicting the postoperative neurological recovery time were 0.794, 0.767, 0.772, and 0.802, respectively. The AUC predicted by the combined detection of each factor was 0.876, which was better than the area under the curve of single prediction. Conclusion. Patients with CSM combined with developmental spinal stenosis were characterized by younger age of onset, a short course of the disease, and linear changes in the spinal cord high signal. The degree of developmental spinal stenosis may affect the postoperative recovery time of neurological function in CSM patients but had little effect on postoperative neurological recovery. The Torg–Pavlov ratio, age of first onset, course of the disease, and changes in the spinal cord linear hyperintensity were the factors that affected postoperative neurological recovery, which may provide a basis for reasonably predicting a postoperative neurological recovery in patients with CSM combined with developmental spinal stenosis.

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“…It protects the cervical intervertebral disc via adjustment of the mechanical features of the soft tissues around the cervical vertebra, relieving stress around the NP and delaying its degeneration [24][25][26]. Our previous study suggested that Ns therapy could inhibit IVDD by modulating the ECM collagen system and improving the altered structure of the NP [27]. Based on the significant effect of NPC apoptosis on IVDD [28,29], it was hypothesized that Ns therapy inhibits NPC apoptosis in CS rats.…”

Section: Introductionmentioning

confidence: 99%

Needle-scalpel therapy inhibits the apoptosis of nucleus pulposus cells via the SDF-1/CXCR4 axis in a rat degenerative cervical intervertebral disc model

Yang,

Liu,

Sun

et al. 2024

Aging

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As a common disease, cervical spondylosis (CS) results from the degeneration of the cervical intervertebral disc. However, there are still no effective clinical strategies for the treatment of this disease. Needle-scalpel (Ns), a therapy guided by traditional Chinese medicine theory, alleviates intervertebral disc degradation and is widely used in the clinic to treat CS. Stromal cell-derived factor-1 (SDF-1) and its receptor CXC receptor 4 (CXCR4) in nucleus pulposus cells play an important role in CS onset and development. This study aimed to explore whether Ns can relieve pain and regulate the SDF-1/CXCR4 axis in nucleus pulposus cells to inhibit apoptosis, thereby delaying cervical intervertebral disc degradation in a rat model of CS. It was found that the Ns-treated groups exhibited higher mechanical allodynia scores than the model group, and H&E staining, MRI, and scanning electron microscopy revealed that Ns therapy inhibited intervertebral disc degeneration. Additionally, Ns therapy significantly inhibited increases in the RNA and protein expression levels of SDF-1 and CXCR4. Furthermore, these treatments alleviated the apoptosis of nucleus pulposus cells, which manifested as a decline in the proportion of apoptotic nucleus pulposus cells and inhibition of the decrease in the levels of Bcl-2/Bax. These findings indicated that Ns mitigated CS-induced pain, inhibited the apoptosis of nucleus pulposus cells, and alleviated intervertebral disc degeneration in CS rats. These effects may be mediated by specifically regulating the SDF-1/CXCR4 signaling axis. Based on these findings, we conclude that Ns might serve as a promising therapy for the treatment of CS.

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Therapeutic evaluation of acupoint stimulation with needle-scapelon on rat model of degenerative cervical intervertebral discs

Cited by 3 publications

References 15 publications

Establishment and Evaluation of Rat Models of Cervical Spondylopathy with Yin Deficiency Syndrome

Establishment and Evaluation of Rat Models of Cervical Spondylopathy with Yin Deficiency Syndrome

Relationship between Severity of Disease and Postoperative Neurological Recovery in Patients with Cervical Spondylotic Myelopathy Combined with Developmental Spinal Stenosis

Needle-scalpel therapy inhibits the apoptosis of nucleus pulposus cells via the SDF-1/CXCR4 axis in a rat degenerative cervical intervertebral disc model

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