Therapeutic exposure of diabetic blood using 532 nm-wavelength low level laser through electrical impedance spectroscopy (EIS).

Abstract

Prior information of blood dielectric properties is important and a valuable tool to examine cell morphological structures, functions and mechanisms of their metabolic systems; however, this is under investigated. The study explores the technique of electrical impedance spectroscopy (EIS) measurements to reveal the proper biostimulation conditions for diabetic blood-low level laser therapy. Dielectric spectroscopy of the diabetic patient’s blood was studied using Impedance analyser (Agilent 4294A) within the range of frequency 40 Hz-30 MHz. Blood biostimulations were done using a 532 nm wavelength portable solid-state laser (diode-pumped). The accurate characterization of irradiated diabetic blood impedance and proper observations at power output of 50 mw within exposure duration of 10-15 minutes yield an efficient biostimulation and positively enable rejuvenation and proliferation of the deformed erythrocytes and stimulate healthy blood condition. The irradiated diabetic blood got rejuvenated and had their electrode polarization (α-dispersion region) stretched from frequency of 40 Hz until within a range of 11.7- 11.9 kHz similar to the healthy human blood. This formed appreciable positive effect of the properly stimulated diabetic blood with significant impedance within the cell structures, cytoplasm and plasma of the blood.