Therapeutic immunomodulation with mesenchymal stromal cells: the need for in vivo clues

Dolcetti, Luigi; Dazzi, Francesco

doi:10.13052/ijts2246-8765.2015.003

Cited by 2 publications

(2 citation statements)

References 91 publications

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“…These features have often been used to explain their "immune privileged" status in allogeneic hosts. Furthermore, BM-MSCs have been shown to inhibit dendritic cell maturation [44], B cell [45] and T cell proliferation [46] and differentiation [47], impair natural killer cell activity [48], as well as support the generation of suppressive immune cells such as: regulatory T cells (Tregs) [43,49], myeloid derived suppressor cells (MDSCs) and tolerogenic dendritic cells (tDCs) [50]. Combination of many soluble factors are meritorious for immunosuppressive activity of BM-MSCs, such as: indoleamine 2,3-dioxygenase (IDO), inducible nitric oxide synthase (iNOS), cyclooxygenases (COX), metabolite prostaglandin E2 (PGE2), tumor necrosis factor α-induced protein 6 (TSG6), transforming growth factor β (TGF-β), soluble form of HLA-G5 [35,44].…”

Section: Immunosuppressive Properties Of Bm-mscsmentioning

confidence: 99%

Mesenchymal Stem Cells from Different Tissues: Immune Status and Activity

Trivanović¹,

Krstić²,

Mojsilović³

et al. 2016

Journal of Immunology and Infectious Diseases

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Mesenchymal stem cells (MSCs) have been found in almost all tissues and due to their regenerative properties represent promising tools in cell-based therapy. Role of MSCs in tissue repair is strongly governed by their interplay with immune cells and regulating factors. In addition to the first and most investigated MSCs isolated from bone marrow (BM-MSCs), other tissues were also reported as abundant sources of MSCs, such as peripheral and menstrual blood, fetal and perinatal tissues, adipose and dental tissue. Although BM-MSCs have been shown to be immunosuppressive in both in vitro and in vivo conditions, multiple evidences indicate that these cells have immunogenic properties, making their immune privileged status at least questionable. Besides their similarity with BM-MSCs, MSCs isolated from alternative sources manifest significant differences in their phenotype, functionality, immune status and activity. Complexity of interactions of BM-MSCs with the immune system is also found for MSCs isolated from other tissues. Due to specific conditions present in different tissues, variability in the immune repertoire of resident MSCs is to be expected and must be taken into consideration during experimental or clinical protocol planning.

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Section: Immunosuppressive Properties Of Bm-mscsmentioning

confidence: 99%

Mesenchymal Stem Cells from Different Tissues: Immune Status and Activity

Trivanović¹,

Krstić²,

Mojsilović³

et al. 2016

Journal of Immunology and Infectious Diseases

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“…These constructions can be classified into two main groups: with and without cells [2]. MSCs can be useful in many pathological conditions, though the mechanism of their action is still unknown [6]. Some problems by these con-structions are slow and insufficient vascularization of large ones [7].…”

Section: Introductionmentioning

confidence: 99%

Human Umbilical Cord Blood Stem Cells Differentiate into Keratinocytes under In Vitro Conditions and Culturing Differentiated Cells on Bacterial Cellulose Film

Yavari¹,

Abolhassani²,

Mohammadnejad³

2016

IJST

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Mesenchymal stem cells (MSC) are capable of self renewing and differentiating into other cell types. Human umbilical cord blood (HUCB) has been investigated as an alternative source to bone marrow. The aim of our examinations was to investigate MSC of HUCB could differentiate into keratinocytes under in vitro conditions. In this study, we examined the differentiation with chemical compounds, then to identify the stem cells are differentiated or not, PCR by using the expression of gene cytokeratin 18 was performed and to examine the proliferation characteristic of differentiated keratinocyte from HUCBMSCs, cultured them on cellulose film. Analysis of PCR confirmed the expression of cytokeratin 18 in keratinocytes; results of our study show that cellulose film isn't a compatible substrate for differentiated keratinocytes. And growth factor, BMP4, with hydrocortisone and ascorbic acid with specific amounts is an appropriate induction factor for inducing of differentiation into keratinocytes.

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Therapeutic immunomodulation with mesenchymal stromal cells: the need for in vivo clues

Cited by 2 publications

References 91 publications

Mesenchymal Stem Cells from Different Tissues: Immune Status and Activity

Mesenchymal Stem Cells from Different Tissues: Immune Status and Activity

Human Umbilical Cord Blood Stem Cells Differentiate into Keratinocytes under In Vitro Conditions and Culturing Differentiated Cells on Bacterial Cellulose Film

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