Therapeutic Potential and Clinical Efficacy of Acid-Resistant Fungal Lipase in the Treatment of Pancreatic Steatorrhoea due to Cystic Fibrosis

Zentler-Munro, P L; Assoufi, B A; Balasubramanian, K.A.; Cornell, Steven H.; Benoliel, Daniel; Northfield, T. C.; Hodson, Margaret E.

doi:10.1097/00006676-199205000-00007

Cited by 64 publications

(30 citation statements)

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“…Promising application fields include waste treatment [3], the resolution of racemic mixtures to produce optically active compounds [4], and the development of characteristic flavors in food processing industries [5]. In the medical and therapeutic fields, they are particularly used as substitutes for pancreatic lipases for the treatment of mucovicidosis and cystic fibrosis [6,7].…”

Section: Introductionmentioning

confidence: 99%

An Enhanced Process for the Production of a Highly Purified Extracellular Lipase in the Non-conventional Yeast Yarrowia lipolytica

Turki

Ayed

Chalghoumi

et al. 2009

Appl Biochem Biotechnol

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Yarrowia lipolytica LgX64.81 is a non-genetically modified mutant that was previously identified as a promising microorganism for extracellular lipase production. In this work, the development of a fed-batch process for the production of this enzyme in this strain was described. A lipolytic activity of 2,145 U/mL was obtained after 32 h of batch culture in a defined medium supplemented with 10 g/L of tryptone, an enhancer of lipase expression. To maximize the volumetric productivity, two different fed-batch strategies had been investigated. In comparison to batch process, the intermittent fed-batch strategy had not improved the volumetric lipase productivity. In contrast, the stepwise feeding strategy combined with uncoupled cell growth and lipase production phases resulted in a 2-fold increase in the volumetric lipase productivity, namely, the lipase activity reached 10,000 U/mL after 80 h of culture. Furthermore, this lipase was purified to homogeneity by anion exchange chromatography on MonoQ resin followed by gel filtration on Sephacryl S-100. This process resulted in an overall yield of 72% and a 3.5-fold increase of the specific lipase activity. The developed process offers a great potential for an economic production of Lip2 at large scale in Y. lipolytica LgX64.81.

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Section: Introductionmentioning

confidence: 99%

An Enhanced Process for the Production of a Highly Purified Extracellular Lipase in the Non-conventional Yeast Yarrowia lipolytica

Turki

Ayed

Chalghoumi

et al. 2009

Appl Biochem Biotechnol

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“…In the medical and therapeutic Welds, they are particularly important as substitutes for pancreatic lipases (Zentler-Munro et al, 1992). The fact that lipases remain active in various organic solvents has allowed their use in the catalysis of inter-and trans-esteriWcation reactions of oils and fats (Kotting and Eibl, 1994).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica

Fickers

Fudalej

Dall

et al. 2005

Fungal Genetics and Biology

103

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“…The biotechnological potential of these enzymes is steadily increasing with various applications such as in the oleochemical, detergent, and food industries, surfactant production, organic chemistry, and fat-containing waste effluent treatment (for reviews see references 15 and 40). Lipases also may be used for human therapy of pancreatic deficiency (48). Y. lipolytica secretes several lipases and esterases.…”

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confidence: 99%

Autocloning and Amplification of LIP2 in Yarrowia lipolytica

Pignède

Wang²,

Fudalej

et al. 2000

Appl Environ Microbiol

136

115

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We synthesized a Yarrowia lipolytica strain overproducing lipase for industrial applications by using long terminal repeat () of the Y. lipolytica retrotransposon Ylt1 and an allele of URA3 with a promoter deletion to construct JMP3. JMP3 is a derivative of plasmid pHSS6 carrying a NotI-NotI cassette which contains a defective URA3 allele, a polylinker sequence, and the region for targeting to multiple sites in the genome of the recipient. We inserted the LIP2 gene (encoding extracellular lipase) under the control of the strong POX2 promoter into JMP3 to generate JMP6. The pHSS6 region was removed by NotI digestion prior to transformation. Two Y. lipolytica strains transformed with the JMP6 LIP2 cassette had a mean of 10 integrated copies devoid of the Escherichia coli region, corresponding to an autocloning event. The copy number in the transformants was stable even after 120 generations in nonselective and lipase-inducing conditions. The resulting strains could produce 0.5 g of active lipase per liter in the supernatant, 40 times more than the single-copy strain with the LIP2 promoter. This work provides a new expression system in Y. lipolytica that results in strains devoid of bacterial DNA and in strains producing a high level of lipase for industrial uses, waste treatment, and pancreatic insufficiency therapy.

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Therapeutic Potential and Clinical Efficacy of Acid-Resistant Fungal Lipase in the Treatment of Pancreatic Steatorrhoea due to Cystic Fibrosis

Cited by 64 publications

References 0 publications

An Enhanced Process for the Production of a Highly Purified Extracellular Lipase in the Non-conventional Yeast Yarrowia lipolytica

An Enhanced Process for the Production of a Highly Purified Extracellular Lipase in the Non-conventional Yeast Yarrowia lipolytica

Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica

Autocloning and Amplification of LIP2 in Yarrowia lipolytica

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