Therapeutic potential of biogenic and optimized silver nanoparticles using Rubia cordifolia L. leaf extract

Abstract

Rubia cordifolia L. is a widely used traditional medicine in the Indian sub-continent and Eastern Asia. In the present study, the aqueous leaf extract of the R. Cordifolia was used to fabricate silver nanoparticles (RC@AgNPs), following a green synthesis approach. Effect of temperature (60 °C), pH (8), as well the concentration of leaf extract (2 ml) and silver nitrate (2 mM) were optimized for the synthesis of stable RC@AgNPs. The phytofabrication of nanosilver was validated by UV–visible spectral analysis, which displayed a distinctive surface plasmon resonance peak at 432 nm. The effective functional molecules as capping and stabilizing agents, and responsible for the conversion of Ag+ to nanosilver (Ag0) were identified using the FTIR spectra. The spherical RC@AgNPs with an average size of ~ 20.98 nm, crystalline nature, and 61% elemental composition were revealed by TEM, SEM, XRD, and. EDX. Biogenic RC@AgNPs displayed a remarkable anticancer activity against B16F10 (melanoma) and A431 (carcinoma) cell lines with respective IC50 of 36.63 and 54.09 µg/mL, respectively. Besides, RC@AgNPs showed strong antifungal activity against aflatoxigenic Aspergillus flavus, DNA-binding properties, and DPPH and ABTS free radical inhibition. The presented research provides a potential therapeutic agent to be utilized in various biomedical applications.

Leucophyllum frutescens mediated synthesis of silver and gold nanoparticles for catalytic dye degradation

“…Likewise the optimum temperature, pH, leaf extract concentration and AgNO 3 concentration were 60 °C, 8, 2 ml and 2 mM, Frontiers in Chemistry frontiersin.org respectively during synthesis of AgNPs employing Rubia cordifolia L. leaf extract. This signifies that the higher temperature can enhance the synthesis of AgNPs (Chandraker et al, 2022b). The yield of AgNPs and AuNPs were calculated as 40 mg and 30 mg from 100 ml reaction mixture, apparent cost of which were 35 (INR) and 311.5 (INR), respectively.…”

The Underling Mechanisms Exploration of Rubia cordifolia L. Extract Against Rheumatoid Arthritis by Integrating Network Pharmacology and Metabolomics

“… 15 The aqueous leaf extract of RC showed antitumor, DNA-binding, antifungal, and antioxidant properties. 16 While the dry rhizome of RC is recorded in the 2020 edition of the Chinese Pharmacopoeia and are widely used as traditional Chinese medicine for the treatment of tuberculosis, contusions, menoxenia, and rheumatism in China, Japan, Korea, and India. 17 Our previous study indicated that the ethanol extract of R. cordifolia (RCE) has significant effects on foot swelling and joint damage.…”

Green synthesis of glucose-capped stable silver nanoparticles: a cost-effective sensor for the selective detection of Hg2+ ions in aqueous solutions

“…Among them, the noble metal nanoparticles, mainly silver nanoparticles (AgNPs), are found to be very promising, due to their versatile properties and application in the field of diagnostic medicine, 12 antimicrobial studies, and therapeutics. [13][14][15][16] Although there are different chemical methods reported for the synthesis of stable AgNPs, the use of the green method has captivated a lot of attention due to its safe synthetic procedures, use of environment-friendly renewable resources, nontoxic solvents, ambient temperature, and pressure, etc. 17 Plant-mediated green-route synthesized AgNPs have many advantages compared to chemically synthesized AgNPs.…”