Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing

Abstract

Present study was conducted to explore the possibility of treating the diabetic rat wounds by canine bone marrow derived mesenchymal stem cells (BMSCs) and its conditioned media (CM). Canine bone marrow stem cells were isolated ascetically from dog and cultured in vitro. Stem cells conditioned media was collected from third passage cells at 120 hours of culture. Streptozotocin was used for induction of diabetes in rats. Six groups were made for the wound healing therapy in which group I, II and III were non-diabetic while group IV, V and VI were diabetic with six animals in each group and one wound was created in each animal. Group II and V received stem cells in culture media and group III and VI received conditioned media while group I and IV were kept as respective controls given only stem cell culture media. Stem cells and its conditioned media were injected at the periphery of wounds. Wound healing was assessed by wound contraction, photographic evaluation at different time interval (0, th day) and histomorphological examination on day 28. The outcomes of wound healing experiment suggested that canine bone marrow stem cells as well as its conditioned media can be exploited xenogenically very well for diabetic rat wound healing.

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

“…The different isolation methods could be responsible for the observed differences in collagen type II expression (Guest et al, 2008). Expression of the genes of haematopoetic stem cell markers (CD34 and CD45) was lacking in present study as reported by others (Guest et al, 2008;Gade et al, 2013;Ansari et al, 2013). However, there are reports that showed MSCs expression of the CD34 and CD45 surface markers, with one report showing their dim expression (Tan et al, 2013) and other reported that MSCs have heterogeneous CD34 and CD45 phenotype that changes under in vitro conditions (Kaiser et al, 2007).…”

“…Isolation of rMSCs was performed by a standard method reported earlier (Pittenger et al, 1999;Woodbury et al, 2002;Ansari et al, 2013;Udehiya et al, 2013). The rMSCs on the basis of morphology, cell frequency rate and molecular marker profile were similar to the canine, murine, porcine, rodent or human MSCs (Castro-Malaspina et al, 1980).…”

Impact of Cryopreservation on Caprine Fetal Adnexa Derived Stem Cells and Its Evaluation for Growth Kinetics, Phenotypic Characterization, and Wound Healing Potential in Xenogenic Rat Model

“…Topical administration of hCB MSCs, and cWJ, MSCs in the cutaneous wounds of mice (diabetic [Liu et al, ] and normal [Tark et al, ]) and rat [Pratheesh et al, ], respectively have exhibited positive effects on wound healing. Wound healing potential of MSCs have also been investigated in mice, rat, rabbit, canine, and human by different groups (McFarlin et al, ; Wu et al, ; Yoshikawa et al, ; Borena et al, ; Ansari et al, ). MSCs improve cutaneous wound healing by stimulating cellular response to injury and promote regeneration rather than scar formation (Rustad et al, ).…”

An allogenic therapeutic strategy for canine spinal cord injury using mesenchymal stem cells

“…Apart from proliferation ability, lineage differentiation potential is another important criterion for characterization of MSCs. MSCs were successfully differentiated in all the three lineages confirming the cell suitability for cartilage and bone‐related injuries (Ansari et al, ). Canine MSCs cultured in bFGF supplemented media get differentiated in neuronal cells (Nakano et al, ).…”