Therapeutic potential of chlorotoxin-like neurotoxin from the Chinese scorpion for human gliomas

Fu, Yuejun; Yin, Litian; Liang, Aihua; Zhang, Chao-Feng; Wang, Wei; Chai, Baofeng; Yang, Jie; Fan, Xiaojun

doi:10.1016/j.neulet.2006.10.056

Cited by 61 publications

(40 citation statements)

References 14 publications

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“…These results agree with previous reports that scorpion venom inhibited the growth of lymphoma (3,9), leukemia (10), neuroblastoma (4), gliomas (11)(12)(13), breast cancer (14) and prostate cancer (15,16).…”

Section: Discussionsupporting

confidence: 93%

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

Song

Zhang

Sun

et al. 2012

Experimental and Therapeutic Medicine

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Abstract. Scorpion venom contains various groups of compounds that exhibit anticancer activity against a variety of malignancies through a poorly understood mechanism. While the aberrant activation of nuclear factor κB (NF-κB) has been linked with hematopoietic malignancies, we hypothesized that scorpion venom mediates its effects by modulating the NF-κB signaling pathway. In the present study, we examined the effects of scorpion venom component III (SVCIII) on the human leukemia cell lines THP-1 and Jurkat and focused on the NF-κB signaling pathway. Our results showed that SVCIII inhibited cell proliferation, caused cell cycle arrest at G1 phase and inhibited the expression of cell cycle regulatory protein cyclin D1 in a dose-dependent manner in THP-1 and Jurkat cells. SVCIII also suppressed the constitutive NF-κB activation through inhibition of the phosphorylation and degradation of IκBα. NF-κB luciferase reporter activity was also inhibited by SVCIII. Our data suggest that SVCIII, a natural compound, may exert its antiproliferative effects by inhibiting the activation of NF-κB and, thus, has potential use in the treatment of hematopoietic malignancies, alone or in combination with other agents.

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Section: Discussionsupporting

confidence: 93%

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

Song

Zhang

Sun

et al. 2012

Experimental and Therapeutic Medicine

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“…Scorpion venom exhibits anticancer potential via the inhibition of cell proliferation and growth in vitro by interfering with the activity of various signaling molecules such as phosphatase and tensin homolog, which are involved in a number of signal transduction cascades (38)(39)(40). The present study evaluated the effects of scorpion venom on cell motility and colony formation in two well-established and characterized colorectal cancer cell lines, HCT-8 and HCT-116, in addition to the breast cancer cell line MDA-MB-231.…”

Section: Discussionmentioning

confidence: 99%

In vitro analysis of the anticancer properties of scorpion venom in colorectal and breast cancer cell lines

2015

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Abstract. Scorpion venom contains various types of proteins and peptides that are able to act as inhibitors of neurotransmitter molecules. This is achieved primarily via the inhibition of ion channels. In addition, scorpion venom has been demonstrated to exhibit anticancer properties in prostate and breast cancer, as well as leukemia. The anticancer properties of scorpion venom are due to its inhibitory effect on matrix metalloproteinase (MMP) activity, which leads to reduced motility and invasion in tumor cells. The inhibitory effects of venom on MMPs additionally lead to a reduction in the metastatic potential of malignant tumors. In the present study, the effect of venom obtained from a local serpentarium facility was examined in colorectal and breast cancer cell lines. Cell motility and clonogenic survival assays revealed a significant decrease (60-90%) in cell motility and colony formation, two significant hallmarks of cancer survival, following treatment with various concentrations of venom. These results were in agreement with previous studies demonstrating the anticancer activity of scorpion venom. In conclusion, the venom utilized at the Research Center of Prince Sultan Military Medical City Hospital (Riyadh, Saudi Arabia) possesses significant anticancer potential against colorectal and breast cancer cell lines.

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“…BmK CT also specifically and selectively binds to and blocks the voltage-gated glioma cell surface chloride channels in a mechanism similar with chlorotoxin, causing cell growth arrest and apoptosis (Fu et al 2007). The inhibition of the growth of C6 glioma cells treated with BmK CT at 2 9 10 -5 mg/ml (*5 lM) was 29% (Zhao et al 2010).…”

Section: Introductionmentioning

confidence: 98%

A potential strategy for high-grade gliomas: combination treatment with lithium chloride and BmK CT

Zheng

Huang

et al. 2011

Biotechnol Lett

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Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mood stabilizer, could synergistically inhibit the migration, invasion and proliferation of C6 glioma cells. The decreased invasiveness of C6 glioma cells was accompanied by inhibited activation, catalytic activity and/or expression of matrix metalloproteinase-2. Moreover, TOPfalsh luciferase reporter and immunofluorescence staining showed altered localization pattern of β-catenin at the leading edge of 2D scratch. Our results suggested that the combination treatment of lithium and BmK CT may constitute a novel and potential strategy for HHG therapy.

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Therapeutic potential of chlorotoxin-like neurotoxin from the Chinese scorpion for human gliomas

Cited by 61 publications

References 14 publications

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

Scorpion venom component III inhibits cell proliferation by modulating NF-κB activation in human leukemia cells

In vitro analysis of the anticancer properties of scorpion venom in colorectal and breast cancer cell lines

A potential strategy for high-grade gliomas: combination treatment with lithium chloride and BmK CT

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