Therapeutic Potential of Extracellular Vesicles in Aging and Age-Related Diseases

Sanz-Ros, Jorge; Mas-Bargues, Cristina; Romero-García, Nekane; Huete-Acevedo, Javier; Dromant, Mar; Borrás, Consuelo

doi:10.3390/ijms232314632

Cited by 12 publications

(7 citation statements)

References 170 publications

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“…It has been well-documented that EVs are involved in aging processes through regulating multiple biogenesis pathways, such as cellular senescence, inflammation, tissue regeneration, oxidative stress, metabolism and autophagy ( 1 , 7 , 19 , 40 , 41 ). We have observed age-associated declines of multiple immune cell-related EV subpopulations in healthy humans and mice, consistent with an immunosenescence phenotype in healthy aging that is reflected in EV subpopulations ( 1 , 6 ).…”

Section: Discussionmentioning

confidence: 99%

Immune system-related plasma extracellular vesicles in healthy aging

Zhang,

Ma,

Huebner

et al. 2024

Front. Immunol.

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ObjectivesTo identify age-related plasma extracellular vehicle (EVs) phenotypes in healthy adults.MethodsEV proteomics by high-resolution mass spectrometry to evaluate EV protein stability and discover age-associated EV proteins (n=4 with 4 serial freeze-thaws each); validation by high-resolution flow cytometry and EV cytokine quantification by multiplex ELISA (n=28 healthy donors, aged 18-83 years); quantification of WI-38 fibroblast cell proliferation response to co-culture with PKH67-labeled young and old plasma EVs. The EV samples from these plasma specimens were previously characterized for bilayer structure, intra-vesicle mitochondria and cytokines, and hematopoietic cell-related surface markers.ResultsCompared with matched exo-EVs (EV-depleted supernatants), endo-EVs (EV-associated) had higher mean TNF-α and IL-27, lower mean IL-6, IL-11, IFN-γ, and IL-17A/F, and similar mean IL-1β, IL-21, and IL-22 concentrations. Some endo-EV and exo-EV cytokine concentrations were correlated, including TNF-α, IL-27, IL-6, IL-1β, and IFN-γ, but not IL-11, IL-17A/F, IL-21 or IL-22. Endo-EV IFN-γ and exo-EV IL-17A/F and IL-21 declined with age. By proteomics and confirmed by flow cytometry, we identified age-associated decline of fibrinogen (FGA, FGB and FGG) in EVs. Age-related EV proteins indicated predominant origins in the liver and innate immune system. WI-38 cells (>95%) internalized similar amounts of young and old plasma EVs, but cells that internalized PKH67-EVs, particularly young EVs, underwent significantly greater cell proliferation.ConclusionEndo-EV and exo-EV cytokines function as different biomarkers. The observed healthy aging EV phenotype reflected a downregulation of EV fibrinogen subpopulations consistent with the absence of a pro-coagulant and pro-inflammatory condition common with age-related disease.

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Section: Discussionmentioning

confidence: 99%

Immune system-related plasma extracellular vesicles in healthy aging

Zhang,

Ma,

Huebner

et al. 2024

Front. Immunol.

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“…Preclinical investigations showed that exosomes may have a therapeutic role in aging and other age-related diseases [99]. Cellular aging is due to various biological changes including, epigenetic alteration, genomic instability, senescence, oxidative stress, mitochondrial decline, and dysregulation of intracellular communication [100].…”

Section: Clinical Applicability Of Exosomes In Photoaging: Current St...mentioning

confidence: 99%

“…Some studies have demonstrated the therapeutic potential of exosome in preclinical models of age-related diseases such as Alzheimer's, Type 2 diabetes (T2DM), osteoarthritis, chronic kidney disease, etc. [99].…”

Section: Clinical Applicability Of Exosomes In Photoaging: Current St...mentioning

confidence: 99%

Exosomes in skin photoaging: biological functions and therapeutic opportunity

Hajialiasgary Najafabadi,

Soheilifar,

Masoudi-Khoram

2024

Cell Commun Signal

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Exosomes are tiny extracellular vesicles secreted by most cell types, which are filled with proteins, lipids, and nucleic acids (non-coding RNAs, mRNA, DNA), can be released by donor cells to subsequently modulate the function of recipient cells. Skin photoaging is the premature aging of the skin structures over time due to repeated exposure to ultraviolet (UV) which is evidenced by dyspigmentation, telangiectasias, roughness, rhytides, elastosis, and precancerous changes. Exosomes are associated with aging-related processes including, oxidative stress, inflammation, and senescence. Anti-aging features of exosomes have been implicated in various in vitro and pre-clinical studies. Stem cell-derived exosomes can restore skin physiological function and regenerate or rejuvenate damaged skin tissue through various mechanisms such as decreased expression of matrix metalloproteinase (MMP), increased collagen and elastin production, and modulation of intracellular signaling pathways as well as, intercellular communication. All these evidences are promising for the therapeutic potential of exosomes in skin photoaging. This review aims to investigate the molecular mechanisms and the effects of exosomes in photoaging.

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“…The reason is that EVs are released by all human cells and are ubiquitous conveyors of intercellular messages and can function as intercellular mediators because of their selective cargoes, such as specific miRNAs, proteins, and lipids [ 94 , 95 ]. They contain a wealth of biomarkers which could be used to monitor the status of some pathologies and clinical conditions [ 96 ]. The exact EV contents could be advantageous in using EVs as a biomarker in developing diseases.…”

Section: Extracellular Vesicles As Biomarkers In Aging and Age-associ...mentioning

confidence: 99%

Extracellular Vesicles as “Very Important Particles” (VIPs) in Aging

Mas-Bargues

Alique

2023

IJMS

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In recent decades, extracellular vesicles have been recognized as “very important particles” (VIPs) associated with aging and age-related disease. During the 1980s, researchers discovered that these vesicle particles released by cells were not debris but signaling molecules carrying cargoes that play key roles in physiological processes and physiopathological modulation. Following the International Society for Extracellular Vesicles (ISEV) recommendation, different vesicle particles (e.g., exosomes, microvesicles, oncosomes) have been named globally extracellular vesicles. These vesicles are essential to maintain body homeostasis owing to their essential and evolutionarily conserved role in cellular communication and interaction with different tissues. Furthermore, recent studies have shown the role of extracellular vesicles in aging and age-associated diseases. This review summarizes the advances in the study of extracellular vesicles, mainly focusing on recently refined methods for their isolation and characterization. In addition, the role of extracellular vesicles in cell signaling and maintenance of homeostasis, as well as their usefulness as new biomarkers and therapeutic agents in aging and age-associated diseases, has also been highlighted.

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Therapeutic Potential of Extracellular Vesicles in Aging and Age-Related Diseases

Cited by 12 publications

References 170 publications

Immune system-related plasma extracellular vesicles in healthy aging

Immune system-related plasma extracellular vesicles in healthy aging

Exosomes in skin photoaging: biological functions and therapeutic opportunity

Extracellular Vesicles as “Very Important Particles” (VIPs) in Aging

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