Therapeutic potential of Ni(II) Schiff base complex on CCl4 toxicity

Abstract

Carbon tetrachloride (CCl4) is a known ecological hazardous xenobiotic that could motivate hepatotoxicity. We aimed to examine, for the first time, the therapeutic potential of nickel(II) diacetyl monoxime-2-pyridyl hydrazone complex versus CCl4induced hepatotoxicity in rats. We used six rat groups of ten animals each. The negative control, vehicle, normal rats injected i.p. with the complex (2.4 mg/kg/day), positive control rats injected i.p. with CCl4, and treated rats administered complex via injection at low and high concentrations of 1.2 and 2.4 mg/kg/day, respectively at the same time as CCl4 injection for shortterm (3-weeks) and long-term (8-weeks) treatment. Intoxicated-rodents exhibited significant elevations in the liver index, hepatic serum markers, and oxidative stress with significant reductions in the hepatic, antioxidants, nucleic acids, and proteins. Complex co-treatment with CCl4 significantly suppressed the elevated liver enzyme activities, attenuated oxidative stress, reactivated antioxidant-system components, and amended the hepatic tissue injury. The complex high dose was more efficient than the low dose. Results of negative control were analogous to those of normal rats injected with the complex high dose. The histopathological analysis also supported the above findings. These results show that complex has good antioxidant and therapeutic properties, which can help in treating and preventing CCl4-induced hepatotoxicity.

Therapeutic effect of date palm extract on ccl4 induced HCC in rat

“…The activation of kupffer cells, which is accompanied by the generation of proinflammatory mediators, is a component of the second stage of CCl4-induced hepatotoxicity [14]. In consistent with previous studies demonstrated the alteration of biochemical markers, hepatic antioxidant status and hepatic nucleic acid content may therefore manifest of oxidative stress and cellular DNA damage caused by CCl4, Enzymatic defence mechanisms such as catalase (CAT), superoxide dismutases (SOD), and gluthatione protect cells from free radical damage (GSH) [15]. As shown in the table(1), we revealed that palm dates increased the levels of glutathione, catalase, and superoxide dismutase when compared to the positive control group Since catalase breaks down hydrogen peroxide into water and oxygen and SOD is in responsible of removing superoxide radicals, these enzymes may help regulate the redox state of plasma.This observation perfectly agrees with those of Ceci et al [16].We focused on the potential chemotherapeutic effects of palm dates in the current study, which may have been mediated through regulating the expression of MMP9 and Caspase3 in an animal model of HCC.…”