Therapeutic potential of snake venom in cancer therapy: current perspectives

Vyas, Vivek K.; Brahmbhatt, Keyur; Bhatt, Hardik; Parmar, Utsav

doi:10.1016/s2221-1691(13)60042-8

Cited by 130 publications

(87 citation statements)

References 53 publications

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“…For instance, recent studies have demonstrated that the usage of various anti-VEGF/PDGF strategies is linked to an increased risk of early metastasis in animal cancer models. Although the clinical relevance of these preclinical studies is not yet clear, these data support the idea that not only is there an imperative need to design novel anti-angiogenic drugs with better anti-invasive properties, also to test the impact of standard of care anti-angiogenics such as Avastin on metastasis [30][31][32][33][34][35][36][37][38][39][40].…”

Section: Cr Venom Of Vn Was Collected Lyophilized Successfullymentioning

confidence: 94%

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A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in Vietnam

Trinh¹,

Thuc²

2016

Anat Physiol

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Aim: Vietnam is a tropical and agricultural country. Annually, there are 30,000 cases of snake bite. Two venomous snake families cause the big medical problem. In this, Calloselasma Rhodostoma (CR) is the most dangerous snake. Therefore, since 2001, the scientific research collaborations between Vietnam (VN) and University of Southern California (USC) were established. Since 2014 this project has been approved by VN government. The aims of the 1st project are to establish the technological process for purification of dsintegrin from CR venom of VN (CRd.VN), to determine the molecular weight, structure and its biological antitumor activities. Methods:The process of collection, lyophilisation of CR venom from VN. Protein concentration of CR venom was determined by BCA assay. High Performance Liquid Chromatography (HPLC), SDS-PAGE, Mass spectrometry (MS) analysis and sequencing by tryptic digestion were used for purification of CRd.VN and its molecular weight (MW) and structure. Standard cell biology methods were employed to characterize CRd's abilities (in vitro) to inhibit platelet aggregation, adhesion, migration and invasion of tumor cells. Its anti-cancer activity in a breast cancer (BC) murine model (in vivo) was tested.Results: Peak 7 of HPLC (CRd.VN), showed a single ~10 kDa band on SDS-PAGE gel. CRd.VN's MW, structure and the sequence are 7.33 kDa, a monomer containing 68 amino acids with an RGD motif (position 49-51) and 6 disulfide bonds. The anticancer activities of CRd.VN are very strong. Conclusion:We have shown that CRd.VN is a possible anti-tumor agent with clinical potential. However, further research is required on CRd recombinant production, preliminary pharmacokinetics/ toxicology properties and antitumor activities. The inhibition of angiogenesis is one of the most heavily explored treatment options for cancer, and snake venom disintegrins represent a library of molecules with different structures, potencies, and specificities and are good starting points for developing anti-angiogenesis therapeutics. Disintegrins first discovered in 1983 [11] and named in 1990 [12]. They are family of disulphide-rich, stable peptides, originally isolated from snake venom, also found in mammalian proteins (ADAMs). Disintegrins bind exclusively to activated integrins on cells, derived from multi-domain proteins by proteolytic processing, contain RGD (or alternate tri-peptide) motif at tip of a flexible 11-amino acid loop that is involved in integrin interaction. Range in size from small to medium to large and homo-and hetero-dimeric. Disintegrins hold a significant translational potential as anticancer agents based on their anti-angiogenic and anti-metastatic effects demonstrated in various experimental settings [13][14][15]. A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in VietnamAlong with metalloproteases, disintegrins are important compounds in most Viperid and Crotalid venoms. Disintegrins represent a family of nontoxic and nonenzymatic low molecular weight (5-10 kDa) RGD-containing...

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Section: Cr Venom Of Vn Was Collected Lyophilized Successfullymentioning

confidence: 94%

“…Therefore, since 2001, the scientific research collaboration between VN and University of Southern California (USC) was established. Since 2014 this project has been approved by VN government [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40]. …”

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confidence: 99%

A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in Vietnam

Trinh¹,

Thuc²

2016

Anat Physiol

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“…It produces toxicity only if contacted with blood. 8 This is very well understood by our ancient Acharya. So that Acharya Vagbhata used snake venom in various diseases which has been elaborated in 48 th chapter of Uttartantra of Ashtanga Sangraha, i.e.…”

Section: Introductionmentioning

confidence: 91%

A Review on Snake Venom: An Unrevealed Medicine for Human Ailments: Great Scope for Pharmaceutical Research

Kadu¹,

Asutkar²,

Chalakh³

2017

Int. J. Res. Ayurveda Pharm.

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Snake venom is the poisonous secretion (saliva), ejected from the poison apparatus of a poisonous snake, during the act of biting. Venoms of the different species of poisonous snakes are complex mixture of a number of proteins, toxic substances or toxins, peptides, enzymes, and non-protein inclusions in varying proportion. Snake venom is harmless if ingested in liquid or crystal form after drying through mouth and it will be excreted unchanged. It produces toxicity only if contacted with blood. This is very well understood by Ayurveda. Therapeutic uses of snake venom in various diseases have been explored by Acharya Vagbhata in 48 th chapter of Uttartantra of Ashtanga Sangraha, i.e. "Vishopayogiya Adhyaya." Forty-seven different Formulations have been mentioned by Acharya vagbhata in which snake venom is pertinently used with other Ayurvedic herbal drugs. Since last two decades, various researches explore the therapeutic potential of snake venom in various diseases like diabetes, high blood pressure, stroke, Neurological diseases such as Parkinson's disease and Alzheimer's disease, Cancer (various types), aging, skin diseases, exce ssive bleeding condition etc. Current review sums up the therapeutic use of snake venom which is mentioned in Ayurveda as well as in contemporary science which provide new thoughts in field of pharmaceutical research where the use of snake venom is beautifully understood by our ancient Acharyas.

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“…Induction of apoptosis is the most important mechanism for many anticancer agents. In fact, an ideal anticancer agent mostly potentiates apoptotic effects in cancer cells [37]. Fluorescence microscopic analysis of cell death showed that treatment of cells with compounds induce more apoptotic cell death rather than necrotic death.…”

Section: Fluorescence Microscopic Analysis Of Cell Deathmentioning

confidence: 99%

Synthesis of hetero annulated isoxazolo-, pyrido- and pyrimido carbazoles: Screened for in vitro antitumor activity and structure activity relationships, a novel 2-amino-4-(3'-bromo-4'-methoxyphenyl)-8-chloro-11 H -pyrimido[4,5- a ]carbazole as an antitumor agent

Murali

Sparkes

Prasad

2017

European Journal of Medicinal Chemistry

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. Synthesis of hetero annulated isoxazolo-, pyrido-and pyrimido carbazoles: Screened for in vitro antitumor activity and structure activity relationships, a novel 2-amino-4-(3'-bromo-4'-methoxyphenyl)-8-chloro-11H-pyrimido [4,5-a] University of Bristol -Explore Bristol Research General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms

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Therapeutic potential of snake venom in cancer therapy: current perspectives

Cited by 130 publications

References 53 publications

A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in Vietnam

A Novel Anti-tumor Protein from Calloselasma Rhodostoma Venom in Vietnam

A Review on Snake Venom: An Unrevealed Medicine for Human Ailments: Great Scope for Pharmaceutical Research

Synthesis of hetero annulated isoxazolo-, pyrido- and pyrimido carbazoles: Screened for in vitro antitumor activity and structure activity relationships, a novel 2-amino-4-(3'-bromo-4'-methoxyphenyl)-8-chloro-11 H -pyrimido[4,5- a ]carbazole as an antitumor agent

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