Therapeutic potential of the methanolic extract of Lepidium sativum seeds on mice infected with Trypanosoma evansi

Al-Otaibi, Mamdooh S.A.; Al‐Quraishy, Saleh; Al-Malki, Esam S.; Abdel‐Baki, Abdel‐Azeem S.

doi:10.1016/j.sjbs.2018.08.031

Cited by 11 publications

(11 citation statements)

References 26 publications

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“…But the rate of increment in parasitemia level was signi cantly very low (P < 0.05) compared with that of the controls. The present study results are in agreement with those of the previous study by Al-Otaibi et al, (10), which reported antitrypanosomal activity of L. sativum against T. evansi. The antitrypanosomal activity of the extract might be attributed to its antioxidant and/or its immune boosting properties (10).…”

Section: Discussionsupporting

confidence: 94%

“…Lepidium sativum 'Fetto' in Amharic is also among the plants claimed to have antiprotozoal activity and/or antitrypanosomal effect in particular. A single dose in vivo test reported by Al-Otaibi et al, (10) concluded that the plant extract had antitrypanosomal activity against T. evansi. Therefore, the present study was targeted to investigate the in vivo antitrypanosomal activity of crude extracts of leaves of C. citratus and seeds of L. sativum at a variable concentration.…”

Section: Introductionmentioning

confidence: 98%

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Antitrypanosomal Activity of Hydromethanol Extract of Leaves of Cymbopogon Citratus and Seeds of Lepidium Sativum: in-vivo Mice Model

Emiru

Eshetu

Gari

et al. 2021

Preprint

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Background: Trypanosomiasis is one of the neglected tropical diseases of both humans and animals which decreases their productivity and causes death in the worst scenario. Unavailability of vaccine, low therapeutic index of trypanocidal drugs, and development of resistance lead to the need for research focused on developing alternative treatment options especially from medicinal plants. The present study was aimed to investigate antitrypanosomal activities of leaves of Cymbopogon citratus and seeds of Lepidium sativum in in vivo mice model. Methods: The plant extracts were prepared by maceration using 80% methanol and reconstituted with 10% dimethyl sulfoxide (DMSO) to have the desired concentration. The test doses were adjusted to 100, 200 and 400mg/kg based on the toxicity profile. The Plants extracts were administered to the respective groups of mice after the 12th day of field isolate T. congolense inoculation for seven consecutive days. The level of parasitemia, body weight, packed cell volume, and differential white blood cell counts were measured.Results: The in vivo test results revealed that both plant extracts had dose dependent antitrypanosomal activity. Both crude extracts showed a significant reduction in parasite load (P<0.05), ameliorate anaemia (increased or prevent the fall of PCV value) (P<0.05), decreased lymphocytosis and increased neutrophil counts (p<0.05) and improved body weight but significant body weight increment (P<0.05) was observed only in C. citratus treated mice compared to the negative and positive controls. Comparative results from all tested parameters showed that the best activities were observed with C. citratus treated groups of mice. Conclusion: The present study concluded that the crude extracts of leaves of C. citratus and seeds of L. sativum had antitrypanosomal effects and can be potential targets for further studies on the development of alternative antitrypanosomal agents.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 98%

Antitrypanosomal Activity of Hydromethanol Extract of Leaves of Cymbopogon Citratus and Seeds of Lepidium Sativum: in-vivo Mice Model

Emiru

Eshetu

Gari

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…But the rate of increment in parasitemia level was significantly very low ( P < 0.05) compared with that of the controls. The present study results are in agreement with those of the previous study by Al-Otaibi et al [ 14 ], which reported antitrypanosomal activity of L. sativum against T. evansi . Unlike the previous study that used single dose and fixed administration and the follow up periods were only for 4 days, the present study tried to show the effect of the extract in variable dose ranges and longer follow-up period (14 days) by considering the undulating nature of the level of parasitemia in the body of animals.…”

Section: Discussionsupporting

confidence: 94%

“…Lepidium sativum ‘Fetto’ in Amharic is also among the plants claimed to have antiprotozoal activity and/or antitrypanosomal effect in particular. A single dose in vivo test reported by Al-Otaibi et al, [ 14 ] concluded that the plant extract had antitrypanosomal activity against T. evansi . Therefore, the present study was targeted to investigate the in vivo antitrypanosomal activity of crude extracts of leaves of C. citratus and seeds of L. sativum at a variable concentration.…”

Section: Introductionmentioning

confidence: 99%

Antitrypanosomal activity of hydromethanol extract of leaves of Cymbopogon citratus and seeds of Lepidium sativum: in-vivo mice model

Emiru

Makonnen

Regassa

et al. 2021

BMC Complement Med Ther

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Background Trypanosomiasis is one of the neglected tropical diseases of both humans and animals which decreases their productivity and causes death in the worst scenario. Unavailability of vaccines, the low therapeutic index of trypanocidal drugs, and the development of resistance lead to the need for research focused on developing alternative treatment options especially from medicinal plants. The present study was aimed to investigate antitrypanosomal activities of leaves of Cymbopogon citratus and seeds of Lepidium sativum in in-vivo mice model. Methods The plant extracts were prepared by maceration using 80% methanol and reconstituted with 10% dimethyl sulfoxide (DMSO) to have the desired concentration. The test doses were adjusted to 100, 200 and 400 mg/kg based on the toxicity profile. The plants extracts were administered to the respective groups of mice after the 12th day of field isolate T. congolense inoculation for seven consecutive days. The level of parasitemia, bodyweight, packed cell volume (PCV), and differential white blood cell counts were measured. Results The in -vivo test results revealed that both plant extracts had dose-dependent antitrypanosomal activity. Both crude extracts showed a significant reduction in parasite load (P < 0.05), increased or prevent the fall of PCV value (P < 0.05), decreased lymphocytosis and increased neutrophil counts (p < 0.05) and improved bodyweight but significant bodyweight increment (P < 0.05) was observed only in C. citratus treated mice compared to the negative and positive controls. Conclusion The present study concluded that the crude extracts of leaves of C. citratus and seeds of L. sativum had antitrypanosomal effects. Both plants extracts reduced parasitemia level, prevented anemia and improved bodyweight of treated mice. Comparative results from all tested parameters showed that the best activities were observed with C. citratus treated groups of mice.

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“…This study also showed that infection with T. evansi induced leukopenia and this was enhanced by treatment with methanol extract. Leukocytosis, which was recorded in our study, is a good indicator of enhancement and strengthening of the immune system by increasing the mean production of WBCs [ 34 ] and increasing mononuclear phagocytic system activity [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Trypanocidal activity of methanol extracts of the hemolymph of Sarcophaga argyrostoma larva against Trypanosoma evansi infected mice

Farghaly

Sadek

2020

Vet World

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Background and Aim: Many natural products worldwide are used for medicinal purposes. Various insect-isolated compounds were investigated in pursuit of new therapeutic agents. This study aimed to compare the effects of methanol extract of hemolymph of Sarcophaga argyrostoma larvae with diminazene aceturate on some hematological and biochemical indices of mice infected with Trypanosoma evansi. Materials and Methods: Sixteen albino mice were randomly divided into four groups, of four mice, which received different treatments: In Group 1 (G1), mice were infected intraperitoneally with 1×104 T. evansi and received no treatment (positive control), in Group 2 (G2), infected mice were treated with 0.5 mL/kg of diminazene aceturate, in Group 3 (G3), infected mice were treated with 0.5 mL/kg methanol extract of the hemolymph of S. argyrostoma larvae, and in Group 4 (G4), uninfected mice received 0.5 ml of distilled water (negative control). In G3, treatment was started 3 days before injecting the parasite, while for the other groups, a single dose of treatment was applied when the parasite appeared in the blood. Results: Mice from G3 showed low parasitemia of 29×104/mm3 4 days post-infection until the infection completely disappeared on the 5th day, which was earlier than for other groups. The results showed that the numbers of red blood corpuscles (red blood cells [RBCs]) and white blood cells (WBCs) per unit volume were significantly different (p<0.05) between the four groups. The highest RBC (9.09×103 cell/ mm3) and WBC (14.30×103 cell/ mm3) counts were recorded in G3, whereas the lowest values of 6.60 and 4.60×103cell/ mm3, respectively, were recorded for G2. In addition, there were significant differences (p<0.05) between the different groups for platelet counts per unit volume, with G3 having the most (943×103 cell/ mm3) and G2 having the least (357×103 cell/ mm3). There was a significant (p<0.05) difference in the indices of biochemical activities between the extract-treated infected groups and the standard drug-treated group. Conclusion: This study suggests that the methanol extract of the hemolymph of S. argyrostoma larva exhibits trypanocidal activity, so it may be exploited as a suitable candidate for the development of trypanocidal drugs.

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Therapeutic potential of the methanolic extract of Lepidium sativum seeds on mice infected with Trypanosoma evansi

Cited by 11 publications

References 26 publications

Antitrypanosomal Activity of Hydromethanol Extract of Leaves of Cymbopogon Citratus and Seeds of Lepidium Sativum: in-vivo Mice Model

Antitrypanosomal Activity of Hydromethanol Extract of Leaves of Cymbopogon Citratus and Seeds of Lepidium Sativum: in-vivo Mice Model

Antitrypanosomal activity of hydromethanol extract of leaves of Cymbopogon citratus and seeds of Lepidium sativum: in-vivo mice model

Trypanocidal activity of methanol extracts of the hemolymph of Sarcophaga argyrostoma larva against Trypanosoma evansi infected mice

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