Therapeutic role of glucogalactan polysaccharide extracted from Agaricus bisporus on trimethyltin chloride induced neuropathy in rats

Hassan, Amal I.; Ghoneim, Mona A. M.; Ibrahim, Rasha Y.M.

doi:10.5897/ajb2015.14501

Cited by 5 publications

(1 citation statement)

References 47 publications

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“…Plant polysaccharides have various pharmacological activities including anti-oxidant, anti-in ammatory, antitumor and immunoregulating effects [9,10]. For example, the polysaccharides from Agaricus bisporus can reduce homocysteine and neurotransmitter content and enhance body's antioxidant capacity to improve brain function [11]. Cordyceps cicada polysaccharides can protect PC12 cells from glutamate excitatory toxicity [12].…”

Section: Introductionmentioning

confidence: 99%

Two polysaccharides in Zanthoxylum bungeanum Maxim with protective effects against Parkinson's disease

Huang

Hui

Gao

et al. 2023

Preprint

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Background Zanthoxylum bungeanum Maxim can alleviate cognitive dysfunction, improve learning and memory impairment in mice. However, there are no reports on structure identification of polysaccharides from Z. bungeanum, and no data on the effects and possible mechanisms of polysaccharides from the plant against Parkinson's disease (PD). Methods In the present study, crude polysaccharides were obtained by hydroextraction and alcoholic precipitation, DEAE-52 and dextran G-75 chromatographic columns were used for the separation and purification. high-performance gel-permeation chromatography, ion chromatography, GC-MS and NMR were used for structure identification. Activity evaluation was performed by CCK-8, ROS, RT-PCR and Western Blotting experiments. Results Two degraded polysaccharides (WZBP-1-2 and WZBP-2) from Z. bungeanum were purified and identified, and their biological activities were evaluated. WZBP-1-2 and WZBP-2 with average molecular weight (Mw) of 10.89 and 18 kDa mainly contained galacturonic acid, and they were homogalacturonans with the main chain of →4)-α-D-GalAp-(1→. Biological investigation showed that WZBP-1-2 and WZBP-2 significantly increased the viabilities of MN9D cells induced by N-methyl-4-phenylpyridinium (MPP+) and reduced ROS levels compared with model groups. Mechanism study showed that WZBP-1-2 and WZBP-2 markedly increased the expression levels of SIRT1 and Nrf2, and inhibited the protein or mRNA levels of NF-κB, TNF-α and IL-6. Conclusion WZBP-1-2 and WZBP-2 from Z. bungeanum may exhibit protective effects against MPP+-induced neuroinflammation and oxidative stress through activation of SIRT1 signaling and should be considered as candidates for the treatment of PD.

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Section: Introductionmentioning

confidence: 99%

Two polysaccharides in Zanthoxylum bungeanum Maxim with protective effects against Parkinson's disease

Huang

Hui

Gao

et al. 2023

Preprint

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Neuroprotective properties of Cantharellus cibarius polysaccharide fractions in different in vitro models of neurodegeneration

Lemieszek¹,

Nunes

Cardoso

et al. 2018

Carbohydrate Polymers

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The Protective Potency of Medemia argun (An Egyptian Palm) Against Oxidative Stress, and Tissue Injury Induced by γ-Radiation in Rats

Abdel-alim

Moussa

El‐Saied

et al. 2022

Preprint

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Radiation damages living cells and affect all biological process in human body. Thereby, there is a magnificent interest for protecting patients from the aspect consequences radiotherapy and specialized professional workers by developing a natural antioxidant bio-drugs for amelioration of radiation hazards. Medemia argun (an Egyptian Palm) is a mysterious from northern Sudan and southern Egypt (Nubian desert oases). In ancient Egypt, Medemia argun (MA) dried dates have found in the famed tomb of Tutankhamun. Globally, this is first study related to the metabolomics and biological efficiency of MA in alleviating the harmful effect of γ-irradiation. This work highlights the ameliorative role of MA ethanolic seed’s extract, as a natural promising functional food ingredient in alleviating radiation hazard via its antioxidative properties, anti-inflammatory, antiapoptoic and cell regeneration abilities. In this search, we have estimated the LD50 of MA ethanolic seed’s extract in vivo using male Wistar albino rats. The obtained results showed that 200 mg/kg b.wt. is the recommended dose. Rats were randomly splited into four groups. Group I: Represent as control (normal rats), that were received normal food and water daily for six weeks. Group II. Rats subjected to gamma radiation (6 Gy), a single dose of whole body after one week of the experiment. Group III. Rats were injected intraperitoneally with 200 mg/kg b.wt. of MA ethanolic seed’s extract twice/week for six weeks. Group IV. Rats subjected to gamma radiation (6 Gy), a single dose of whole body after one week of the experiment, then injected intraperitoneally with MA ethanolic seed’s extract (200 mg/kg b.wt.) twice/week for six weeks. Each group contains 15 rats. γ-radiation treatment caused a significant increase in DNA fragmentation, NO, MDA, inflammatory biomarkers (TNF-α, HsP70, IL-6, IL-10, 8-OH-dG, CASP-3, MPC-1, and MMP-9), and liver function (ALT, AST, GGT, ALP, and AFP). Also, there is a significant decrease in GSH, SOD, CAT, POD, T. protein, and albumin. Conversely, MA (200 mg/Kg b.wt.) treatment for 6 weeks effectively reflects most of the altered measurements induced by γ-radiation. The potent therapeutic efficacy of MA was manifested in repairing the DNA fragmentation induced by γ-irradiation and this improvement confirmed by decrease in the concentration of 8-OH-dG. To assess the biological activities and beneficial effect of the ethanolic extract of MA seed’s in ameliorating the radiation hazards in rats, phytochemical analysis, were estimated which revealed presence of many beneficial natural health compounds such as, polyphenols (phenolics and flavonoids), proanthocyanidin (condensed tannins), saponins, protein and carbohydrate. Furthermore, histopathological examinations showed significant adverse deleterious changes in the structure of liver tissue due to exposure to γ-radiation, while treatment with MA maintains the cellular structure of the liver without the appearance of any changes. Biochemical data came to agree with the histopathological observations. There are no published reports of the radioprotective role of Medemia argun in rats. Our results showed that MA can be used during radiotherapy as a natural therapeutic drug due to its valuable nutritional benefits, safe, nature and low cost.

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Therapeutic role of glucogalactan polysaccharide extracted from Agaricus bisporus on trimethyltin chloride induced neuropathy in rats

Cited by 5 publications

References 47 publications

Two polysaccharides in Zanthoxylum bungeanum Maxim with protective effects against Parkinson's disease

Two polysaccharides in Zanthoxylum bungeanum Maxim with protective effects against Parkinson's disease

Neuroprotective properties of Cantharellus cibarius polysaccharide fractions in different in vitro models of neurodegeneration

The Protective Potency of Medemia argun (An Egyptian Palm) Against Oxidative Stress, and Tissue Injury Induced by γ-Radiation in Rats

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