Therapeutic transfusions of granulocytes collected by simple bag method for children with cancer and neutropenic infections: results of a single‐centre pilot study

Kikuta, Atsushi; Ohto, Hitoshi; Nemoto, Kenji; Mochizuki, Kazuhiro; Sano, Hideki; Ito, Masaki; Suzuki, Hisamitsu

doi:10.1111/j.1423-0410.2006.00776.x

Cited by 22 publications

(26 citation statements)

References 22 publications

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“…This process is repeated two or three times, when necessary. It is noteworthy that the bag separation method does not require the use of an RBC-sedimenting agent, such as high-MW HES, which reduces the burden on the donor [17,18].…”

Section: Bag Separation Methodsmentioning

confidence: 99%

“…The simple 'bag separation method' without apheresis may be recommended for granulocyte collection in pediatric patients [17]. In brief, whole blood (200 or 400 ml) is drawn into the main bag of a triple-collection bag [200-or 400-ml capacity containing 34 or 68 ml, respectively, of citrate-phosphate-dextrose (CPD) solution] employing the gravity-flow principle.…”

Section: Bag Separation Methodsmentioning

confidence: 99%

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Guidelines for safety management of granulocyte transfusion in Japan

et al. 2010

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Granulocyte transfusion (GTX) has recently been revived by the ability to stimulate granulocyte donors with granulocyte colony-stimulating factor (G-CSF), resulting in a greatly increased number of cells that can be collected. However, there is a paucity of guidelines for assessing the appropriateness and safety management of GTX. The objective of this study was to establish guidelines for the safety management of GTX appropriate for the clinical situation in Japan. The Japan Society of Transfusion Medicine and Cell Therapy, Granulocyte Transfusion Task Force issued the first version of guidelines for GTX considering the safety management of both granulocyte donors and patients who receive GTX therapy. The current guidelines cover issues concerning: (1) the appropriateness of medical institutions, (2) management of granulocyte donors, (3) quality assurance of granulocyte concentrates, (4) administration of granulocyte concentrates, (5) evaluation of the effectiveness of GTX therapy, and (6) complications of GTX therapy. The simple 'bag separation method' without apheresis may be recommended for granulocyte collection in pediatric patients. The first version of guidelines for GTX therapy has been established, which may be appropriate for the clinical situation in Japan. Care should be taken to perform the safety management of both granulocyte donors and patients who receive GTX therapy.

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Section: Bag Separation Methodsmentioning

confidence: 99%

Section: Bag Separation Methodsmentioning

confidence: 99%

Guidelines for safety management of granulocyte transfusion in Japan

et al. 2010

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“…Kikuta et al () conducted a pilot study of GTX collected from G‐CSF‐stimulated blood relatives without apheresis. Only 2 of 13 patients had fungal infections (one with disseminated C. albicans , who died on day 3, and one with oral A. flavus , who survived to day 30).…”

Section: Clinical Efficacymentioning

confidence: 99%

Granulocyte transfusions in the management of invasive fungal infections

et al. 2017

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SUMMARY Granulocyte transfusions have a long history of being used in patients with neutropenia or neutrophil dysfunction to prevent and treat invasive fungal infections. However, there are limited and conflicting data concerning its clinical effectiveness, considerable variations in current granulocyte transfusion practices, and uncertainties about its benefit as an adjunct to modern antifungal therapy. In this review, we provide an overview on granulocyte transfusions and summarize the evidence on their role in the prevention and treatment of invasive fungal infections.

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“…Using this bag separation method, we can decrease the number of donors required for one series of GTX. We have also demonstrated the clinical efficacy of GTX with this bag separation method (Kikuta et al , 2006).…”

mentioning

confidence: 91%

“…Moreover, CFL can only be performed in a limited number of institutions, namely, those that have a continuous‐flow blood cell separator. To address this problem, we have investigated the applicability of a bag separation method (Kikuta et al , 2006; Nemoto et al , 2006). The bag separation method is a simple granulocyte collection procedure.…”

mentioning

confidence: 99%

Extended storage of granulocyte concentrates mobilized by G‐CSF with/without dexamethasone and collected by bag separation method

Mochizuki

Kikuta

Ohto

et al. 2007

Transfusion Medicine

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The aim of this study was to examine the extended storage of granulocyte concentrates mobilized by granulocyte-colony-stimulating factor (G-CSF) with/without dexamethasone (DEX) and collected by a bag separation method. Ten healthy adult volunteers donated blood three times: twice after granulocyte mobilization by (1) injecting G-CSF at 3 microg kg(-1) subcutaneously (s.c.) and (2) injecting G-CSF at 3 microg kg(-1) s.c. + DEX at 8 mg per oral and once (3) for a baseline control without any forms of mobilization. Granulocytes were collected by a bag separation method. The functions (phagocytosis and oxidative killing levels), viability and levels of interleukin (IL)-1beta, IL-8, IL-6 and tumour necrosis factor-alpha of granulocytes were measured. The average numbers of granulocytes collected from 200-mL samples of whole blood from the G-CSF and G-CSF + DEX groups were 35.1 x 10(8) and 49.4 x 10(8), respectively. Phagocytosis level, oxidative killing level and the viability of the granulocytes mobilized by G-CSF with/without DEX were well maintained for up to 72 h of storage after collection. The levels of the cytokines increased in a time-dependent manner. The in vitro phagocytosis level, oxidative killing level and the viability of granulocytes mobilized by G-CSF with/without DEX and collected by bag separation method can be maintained for as long as 72 h after collection.

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Therapeutic transfusions of granulocytes collected by simple bag method for children with cancer and neutropenic infections: results of a single‐centre pilot study

Abstract: The bag method without apheresis relieves the physical load of donors and enables patients with a low body weight to provide an adequate dose of granulocytes.

Cited by 22 publications

References 22 publications

Guidelines for safety management of granulocyte transfusion in Japan

Guidelines for safety management of granulocyte transfusion in Japan

Granulocyte transfusions in the management of invasive fungal infections

Extended storage of granulocyte concentrates mobilized by G‐CSF with/without dexamethasone and collected by bag separation method

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