Therapeutic treatment with fluoxetine using the chronic unpredictable stress model induces changes in neurotransmitters and circulating miRNAs in extracellular vesicles

Estévez-Cabrera, M. Maetzi; Sánchez‐Muñoz, Fausto; Pérez‐Sánchez, Gilberto; Pavón, Lenin; Hernández-Diazcouder, Adrian; Cortes-Altamirano, J. Luis; Soria-Fregoso, C.; Alfaro-Rodríguez, Alfonso; Bonilla-Jaime, Herlinda

doi:10.1016/j.heliyon.2023.e13442

Cited by 5 publications

(2 citation statements)

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“…23,24 These EVs are membrane vesicles with 30-100 nanometers, which transfer specific sets of functional RNAs (mRNAs and noncoding RNAs) and proteins to recipient cells. [25][26][27] In light of existing research, miRNAs secreted from cells within EVs can be transferred to other cell compartments which essentially function as efficient therapeutic molecules for neuropathic pain. 28 Identifying specific miRNAs and their roles related to neuroinflammation and chronic pain would give us better understanding about the mechanisms of IC/BPS therapeutic action, facilitating the development of cell-free therapeutic strategy for IC/BPS therapy.…”

Section: Introductionmentioning

confidence: 99%

“…As a promising cell‐free therapeutic tool, MSCs‐derived EVs can simulate almost all the biological characteristics of MSCs, such as mediation of inflammatory response, tissue damage, and regeneration 23,24 . These EVs are membrane vesicles with 30‐100 nanometers, which transfer specific sets of functional RNAs (mRNAs and noncoding RNAs) and proteins to recipient cells 25–27 . In light of existing research, miRNAs secreted from cells within EVs can be transferred to other cell compartments which essentially function as efficient therapeutic molecules for neuropathic pain 28 .…”

Section: Introductionmentioning

confidence: 99%

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MiR‐9‐enriched mesenchymal stem cells derived exosomes prevent cystitis‐induced bladder pain via suppressing TLR4/NLRP3 pathway in interstitial cystitis mice

Cui,

Bi,

Zhang

et al. 2024

Immunity Inflam & Disease

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BackgroundInflammatory response of central nervous system is an important component mechanism in the bladder pain of interstitial cystitis/bladder pain syndrome (IC/BPS). Exosomes transfer with microRNAs (miRNA) from mesenchymal stem cell (MSCs) might inhibit inflammatory injury of the central nervous system. Herein, the purpose of our study was to explore the therapeutic effects by which extracellular vesicles （EVs） derived from miR‐9‐edreched MSCs in IC/BPS and further investigate the potential mechanism to attenuate neuroinflammation.MethodsOn the basis of IC/BPS model, we used various techniques including bioinformatics, cell and molecular biology, and experimental zoology, to elucidate the role and molecular mechanism of TLR4 in regulating the activation of NLRP3 inflammasome in bladder pain of IC/BPS, and investigate the mechanism and feasibility of MSC‐EVs enriched with miR‐9 in the treatment of bladder pain of IC/BPS.ResultsThe inflammatory responses in systemic and central derived by TLR4 activation were closely related to the cystitis‐induced pelvic/bladder nociception in IC/BPS model. Intrathecal injection of miR‐9‐enreched MSCs derived exosomes were effective in the treatment of cystitis‐induced pelvic/bladder nociception by inhibiting TLR4/NF‑κb/NLRP3 signal pathway in central nervous system of IC/BPS mice.ConclusionsThis study demonstrated that miR‐9‐enreched MSCs derived exosomes alleviate neuroinflammaiton and cystitis‐induced bladder pain by inhibiting TLR4/NF‑κb/NLRP3 signal pathway in interstitial cystitis mice, which is a promising strategy against cystitis‐induced bladder pain.

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