Therapeutic ultrasound ameliorates hyperalgesia and edema on CFA-induced persistent inflammatory response in mice

Rodrigues, Mariana; Barbosa, Rafael Inácio; Neves, Laís Mara Siqueira das; Kuriki, Heloyse Uliam; Gonçalves, Elaine Cristina Dalazen; Santos, Adair Roberto Soares; Dutra, Rafael Cypriano; Marcolino, Alexandre Márcio

doi:10.1016/j.bjane.2022.08.004

Cited by 1 publication

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“…This severe irritation lasts for three to four days (Cinzia et al, 2019) which begins to show a rise in blood neutrophil and leukocyte numbers on the 4th day following CFA injection, as well as erythrocyte sedimentation rate (ESR) values. At 1-2 weeks after the CFA injection, formation of oedema and hyperalgesia in the foot (Rodrigues et al, 2020). Aim of work: It was aimed to assess the effect of complete Freund's adjuvant for induction of rheumatoid arthritis and its effect on some biochemical parameter and some signaling transducers for genetic study.…”

Section: Introductionmentioning

confidence: 99%

Biochemical, Molecular and Histopathological changes of rheumatoid arthritis induced experimentally in rats

Amer

Elhamid

Ali

et al. 2023

Benha Veterinary Medical Journal

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Complete Freund's adjuvant (CFA) is a mixture of desiccated mycobacterium in mannide monooleate and paraffin oil that causes tissue death, lesions, and inflammation. Immunologists have been inducing inflammatory conditions and autoimmune diseases in laboratory animals using CFA for more than 50 years. Rheumatoid arthritis (RA) is a disease marked by ongoing inflammation that affects more than just joints. Twenty male rats were divided into two groups (10 each), Normal control group: Rats were given no medication. CFA-induced arthritis group: Rats were injected with Complete Freund's adjuvant (0.1 ml, once). After 60 days blood samples and Stifle joints were collected for biochemical assay and genetic study of some signaling transducers including IFNγ, Interleukin-4, STAT3 and Interleukin-12. The results revealed that CFA induced rheumatoid arthritis in rats causing an increase in serum liver enzymes and serum concentration of creatinine, urea, FBS, RF, Anti-CCP and CRP, while decreasing in serum total antioxidant capacity (TAC) concentration. Moreover, there was up regulation of IL-12, IFNγ and STAT3 gene, and down regulation of IL-4 gene in CFA-induced arthritis group contrasted with the control group. On conclusion, CFA injection into leg footpad of rats induced arthritic animal model associated with similar inflammatory changes recorded in RA patients IFNγ IL-12 IL-4 Rheumatoid arthritis STAT3

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Section: Introductionmentioning

confidence: 99%