Therapeutic ultrasound experiments in vitro: Review of factors influencing outcomes and reproducibility

Sněhota, Martin; Vachutka, Jaromír; Haar, Gail ter; Dolezal, L; Kolářová, Hana

doi:10.1016/j.ultras.2020.106167

Cited by 35 publications

(22 citation statements)

References 136 publications

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“…Inappropriate setups generate uncertainty in the exposure that for in vitro cell stimulation can exceed 700% . For example, a vast majority of systems currently used in this field exploit nontransparent materials ( e.g. , standard plastic Petri dishes) along the US beam path: they reflect part of the energy and may produce some hardly predictable effects, such as standing wave formation .…”

Section: Discussionmentioning

confidence: 99%

Piezoelectric Nanomaterials Activated by Ultrasound: The Pathway from Discovery to Future Clinical Adoption

et al. 2021

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Electrical stimulation has shown great promise in biomedical applications, such as regenerative medicine, neuromodulation, and cancer treatment. Yet, the use of electrical end effectors such as electrodes requires connectors and batteries, which dramatically hamper the translation of electrical stimulation technologies in several scenarios. Piezoelectric nanomaterials can overcome the limitations of current electrical stimulation procedures as they can be wirelessly activated by external energy sources such as ultrasound. Wireless electrical stimulation mediated by piezoelectric nanoarchitectures constitutes an innovative paradigm enabling the induction of electrical cues within the body in a localized, wireless, and minimally invasive fashion. In this review, we highlight the fundamental mechanisms of acoustically mediated piezoelectric stimulation and its applications in the biomedical area. Yet, the adoption of this technology in a clinical practice is in its infancy, as several open issues, such as piezoelectric properties measurement, control of the ultrasound dose in vitro , modeling and measurement of the piezo effects, knowledge on the triggered bioeffects, therapy targeting, biocompatibility studies, and control of the ultrasound dose delivered in vivo , must be addressed. This article explores the current open challenges in piezoelectric stimulation and proposes strategies that may guide future research efforts in this field toward the translation of this technology to the clinical scene.

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Section: Discussionmentioning

confidence: 99%

Piezoelectric Nanomaterials Activated by Ultrasound: The Pathway from Discovery to Future Clinical Adoption

et al. 2021

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“…To understand the physical phenomena, laboratory tests are performed on cells placed in Petri dishes and insonified by acoustic waves. Unfortunately, as mentioned in [13], several parameters are liable to compromise the monitoring of the acoustic dose delivered to the cells. Among them, the distance between the transducer and the Petri dish, the coupling medium (degassed water), and the formation of standing waves inside the culture medium.…”

Section: Discussionmentioning

confidence: 99%

“…The formation of standing waves inside the Petri dish of the in vitro LIUS device is a challenging problem that prevents accurate prediction of exposure conditions of the cells. This limitation of numerous in-vitro ultrasound stimulation protocols is often mentioned in the literature [11][12][13] and some technical solutions are proposed [14][15][16][17] but without examining their effectiveness, except a very recent paper [10].…”

Section: Introductionmentioning

confidence: 99%

Anti-reflection cover to control acoustic intensity in in vitro low-intensity ultrasound stimulation of cells

et al. 2022

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Low-intensity ultrasound stimulation is a technique used in therapeutic ultrasound for bone regeneration. However, the underlying mechanisms are still poorly understood. In vitro studies on cell cultures are implemented to understand the processes involved. To analyze the effects of ultrasonic waves on cells, the control of the delivered acoustic intensity is essential. However, depending on the insonification protocol chosen, multiple reflections and standing waves that form inside the culture medium strongly hinder the estimates. In this work, we propose the development and the experimental validation of an anti-reflection cover. We demonstrate that this custom-designed device is effective in avoiding multiple reflections and makes it possible to artificially replace the layer of culture medium with a large amount of water. Finally, an analytical study of the acoustic intensity delivered to the cells is proposed.

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“…Although ultrasound stimulation was found to promote sEV yields of various cell types, the poor reproducibility of these research results hindered the practical application of ultrasound. It mainly resulted from the uncertainty in the multiple ultrasound field parameters experienced by sonicated donor cells [ 112 ]. Therefore, it is necessary to develop more stable and controllable equipment for ultrasound output.…”

Section: Current Strategies To Regulate the Production And Biological Function Of Sevsmentioning

confidence: 99%

Regulating the production and biological function of small extracellular vesicles: current strategies, applications and prospects

Luo

Wang

et al. 2021

J Nanobiotechnol

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Numerous studies have confirmed the great application potentials of small extracellular vesicles (sEVs) in biological medical field, especially in tissue repair and regeneration. However, the production capability of sEVs by noncancerous cells is very limited, while their dosage requirements in disease treatments are usually very high. Meanwhile, as cell aging, the sEV production capability of cells decreases and the biological function of sEVs changes accordingly. In addition, for special applications, sEVs carrying desired bioactive substances should be designed to perform their expected biological function. Therefore, improving the production of sEVs and precisely regulating their biological function are of great significance for promoting the clinical applications of sEVs. In this review, some of the current classic strategies in affecting the cellular behaviors of donor cells and subsequently regulating the production and biological function of their sEVs are summarized, including gene engineering methods, stress-inducing conditions, chemical regulators, physical methods, and biomaterial stimulations. Through applying these strategies, increased yield of sEVs with required biological function can be obtained for disease treatment and tissue repair, such as bone regeneration, wound healing, nerve function recovery and cancer treatment, which could not only reduce the harvest cost of sEV but promote the practical applications of sEVs in clinic. Graphical Abstract

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Therapeutic ultrasound experiments in vitro: Review of factors influencing outcomes and reproducibility

Cited by 35 publications

References 136 publications

Piezoelectric Nanomaterials Activated by Ultrasound: The Pathway from Discovery to Future Clinical Adoption

Piezoelectric Nanomaterials Activated by Ultrasound: The Pathway from Discovery to Future Clinical Adoption

Anti-reflection cover to control acoustic intensity in in vitro low-intensity ultrasound stimulation of cells

Regulating the production and biological function of small extracellular vesicles: current strategies, applications and prospects

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