Therapy of the experimental infection by Strongyloides venezuelensis in rats with injectable ivermectin or levamizole

Campos, Renato de; Pintó, Pedro; Neto, Vicente Amato; Matsubara, L; Miyamoto, Akimi; Carvalho, Silvino Alves de; Takiguti, Clovis Kiomitsu; Moreira, Antonio Augusto Baillot

doi:10.1590/s0036-46651989000100010

Cited by 6 publications

(3 citation statements)

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“…Strongyloides venezuelensis (Brumpt, 1934) (SVZ), a parasitic nematode of rodents, has been used recently as a suitable parasite model for the studies of biology [21,27,30,32], immunology [8,11,16], morphology [9,12,32], biochemical characteristic [31,33], worm expulsion mechanism [2,13], and nematocidal activity [7,20,22,28]. Our previous studies on SVZ have been made to establish the foundation of an in vitro cultivation technique [1,3] and described that Mongolian gerbil was a suitable carrier animal model for persistent infection [4].…”

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confidence: 99%

Migration of Strongyloides venezuelensis in Rats after Oral Inoculation of Free-Living Infective Larvae

Matsuda

Kim

Whang

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Strongyloides venezuelensis (SVZ) infection was chronologically monitored in 85 Sprague-Dawley rats (SDR), which were orally inoculated with approximately 1,000 infective larvae. In order to describe the characteristics of migrating larvae (MLS) in various visceral organs (the liver, lung, cardiac blood, and small intestine), 5 SDR were sacrified at 20 min, 45 min, 1 hr, 2 hr, 3 hr, 4 hr, 8 hr, 12 hr, 16 hr, 48 hr, 72 hr, 96 hr, 120 hr, 144 hr, 168 hr and 192 hr post inoculation (PI). MLS were recovered from the liver a nd blood 20 and 45 min PI and measured 788 ± 26 µm and 846 ± 40 µm in length, respectively. MLS were first observed in the lung tissue 45 min PI and measured 925 ± 38 µm on the average. In the trachea, MLS measuring 849 ± 75 µm appeared 3 to 96 hrs PI. Adult worms (AWS) measuring 1,926 ± 521 µm to 2,956 ± 159 µm in length were observed in the small intestine from 120 hr PI. The worms appeared to mature more than 168 hr PI and attained the average maximum length of 2,420 ± 532 µm. At 3 hr PI focal hyperemic and necrotic lesions were evidently observed in the liver and lung, together with eosinophilic infiltration in the stomach, liver, and lung. The parasites were histologically detectable in the lung tissues but were very difficult to find in the liver and the epithelial layer of small intestine. These data demonstrate that SVZ parasites take 20 min to reach the liver via the stomach and only three hours to reach the trachea through the same route. The development from eggs to adults takes 168 hr in the SDR model. This strongylid invades the host by skin penetration, migrates to the lungs where it molts to the 4th stage larvae, and then travels to the small intestine where it finally molts to mature adults [5,29,35]. The morphologic and kinetic time-lines, however, have not been fully elucidated. Takamure [27] observed intracorporal distribution of migrating filarial parasites (MFP) in Wistar rats and ddy mice from 24 to 96 hr after subcutaneous inoculation, and reported that the MFP were found in the subcutaneous tissue and muscles of rats for 42 hr after exposure. He also described the phenomenon of "concentration" of MFP in the lungs from 45 hr PI adult worms (AWS) appeared in the small intestine just 60 hr PI. On the contrary, the intracorporal migrating course of MLS has not been fully described in orally inoculated animals except the observation that the recovery rate of AWS was lower in the case of oral than in subcutaneous or percutaneous inoculation [26].Strongyloidiasis is a scarcely studied parasite that infects no fewer than 100 million people worldwide, generally in the regions between latitudes 35°N and 30°N. The disease is predominantly distributed in warm moist areas because such climates are suitable for the survival of the larval stage [14,18]. The soil-transmitted nematode, SVZ, occurs in two developmental stages: the free living and parasitic stages. When SVZ infects various species of animals (Mesocricetus auratus, Phodopus campbelli, Cricetulus griseus, Tscherskia ...

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mentioning

confidence: 99%

Migration of Strongyloides venezuelensis in Rats after Oral Inoculation of Free-Living Infective Larvae

Matsuda

Kim

Whang

et al. 2003

The Journal of Veterinary Medical Science

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“…Em outro estudo foram utilizados a ivermectina e o levamisol na forma injetável também em ratos experimentalmente infectados pelo S. venezuelensis, e demonstrou a eficácia tanto para a forma larvária quanto para a forma adulta do parasita. Sendo que a ivermectina demonstrou uma discreta superioridade para a fase larvária enquanto o segundo para os parasitas adultos (Campos et al, 1989).…”

Section: Tratamentounclassified

“…Em humanos, a ivermectina é atualmente mais utilizada para o tratamento da infecção oncocercótica e comumente utilizada contra ectoparasitas. E de acordo com os autores, pode-se cogitar a possibilidade da prescrição deste fármaco e também do levamisol na terapêutica destinada a enfermos parasitados pelo S. stercoralis, sobretudo na sua forma grave (Campos et al 1989;Amato Neto et al 1997;Carranza-Rodríguez et al, 2010).…”

Section: Tratamentounclassified

Efeito anti-helmíntico dos extratos etanólicos das folhas de diferentes espécies de árvores frutíferas sobre Strongyloides venezuelensis in vitro

Allegretti¹

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Dedico,À Deus que sempre esteve presente, tornando superáveis os momentos difíceis e transformando os momentos de felicidade em inesquecíveis e por todas as oportunidades concedidas durante este percurso na minha vida. À minha querida mãe, Maria Aparecida Duart por todo amor, dedicação, confiança, conselhos, paciência e fé. A senhora sabe o quanto é grande o meu amor e o quanto é importante na minha vida! Aos meus padrinhos, Luiz Carlos Matthes In memorian e Neuza Matthes, vocês foram sem dúvida, as pessoas mais importantes na minha formação pessoal e profissional. v AGRADECIMENTOS Á Profª. Silmara Marques Allegretti, pela amizade, oportunidade, confiança, orientação, dedicação, incentivo e apoio durante todo este período de mestrado. E sem dúvida, por ter despertado desde a graduação o interesse e paixão pela parasitologia. Muito obrigada! Aos professores que fizeram parte do exame prévio deste trabalho: Marlene Tiduko Ueta, Luiz Augusto Magalhães, Mara Cristina Pinto, que com suas sugestões e análises críticas contribuíram valorosamente para o desenvolvimento deste trabalho.Ao Prof. Arício Xavier Linhares, pelo auxílio nas análises estatísticas.

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Parasites of Rats and Mice

Baker¹

2007

Flynn's Parasites of Laboratory Animals

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Therapy of the experimental infection by Strongyloides venezuelensis in rats with injectable ivermectin or levamizole

Cited by 6 publications

References 8 publications

Migration of Strongyloides venezuelensis in Rats after Oral Inoculation of Free-Living Infective Larvae

Migration of Strongyloides venezuelensis in Rats after Oral Inoculation of Free-Living Infective Larvae

Efeito anti-helmíntico dos extratos etanólicos das folhas de diferentes espécies de árvores frutíferas sobre Strongyloides venezuelensis in vitro

Parasites of Rats and Mice

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