Therapy of Virus-Infected Plants by Heat Treatment I. Some Properties of Tomato Aspermy Virus and Its Inactivation at 36°C

Johnstone, G. R.; Wade, GC

doi:10.1071/bt9740437

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…2), 29 and 30 "C ( Table I). This would appear to be consistent with other reports that the proportion of plants 'cured' by thermal therapy was sometimes less with longer than shorter treatment periods (Welsh & Nyland, 1965; Mellor & Stace-Smith, 1970; Johnstone & Wade, 1974).…”

Section: Discussionsupporting

confidence: 92%

High temperature inactivation of cucumber and alfalfa mosaic viruses in Nicotiana rustica cultures

Walkey¹

1976

Ann Applied Biology

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SUMMARY Cucumber mosaic (CMV) and alfalfa mosaic (AlfMV) viruses could not be detected in Nicotiana rustica tissues cultured at 32 °C for 16–18 days or at 40 °C for 5 days, but infectivity remained high in comparable tissue cultured at 22 °C. Incubation of infected cultures at 28–30 °C resulted in an initial reduction followed by a partial recovery in the infectivity of both viruses. The infectivity of CMV in tissues grown between 12 and 32 °C was highest in cultures grown at 12 °C. Although CMV infectivity was not detected in cultures after 16–18 days at 32 °C, virus was eliminated only after a further 30 days at 32 °C. When cultures were transferred from 32 to 22 °C after shorter treatment periods, infectivity rapidly increased to levels higher than those of infected tissues grown continuously at 22 °C. At 40 °C, CMV was eliminated from infected tissues after 9 days and AlfMV after 7 days. Cultures grown continuously at 40 °C deteriorated rapidly but, when grown under diurnal alternating periods of 8 h at 40 °C and 16 h at 22 °C, they remained viable and CMV was also inactivated.

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Section: Discussionsupporting

confidence: 92%

High temperature inactivation of cucumber and alfalfa mosaic viruses in Nicotiana rustica cultures

Walkey¹

1976

Ann Applied Biology

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“…At 40°C C MV was eradicated from cultures after nine days treatment. This hypothesis may explain reports that the proportion of plants 'cured' by thermotherapy was sometimes less with longer, than with shorter treatment periods (Mellor and Stace-Smith, 1970;Johnstone and Wade, 1974a). After such treatment virus infectivity was as much as two-and-halftimes greater than in the infected control tissues grown continually at 22°C, although it gradually fell back to the level of the control material over a three to four week period at the lower temperature.…”

Section: Mechanism Of Virus Eradication By High Temperaturementioning

confidence: 98%

Applied Plant Virology

Walkey¹

1991

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“…The absence of strong electrostatic repulsion in the capsid leading to its structural stability in the neutral pH range makes sense biologically; the virion is known to use the sap of a healthy tobacco plant of pH 6.2 as a means for circulating through the plant in attempt to find other mechanically damaged cells to infect. 42 The buildup of a fairly uniform negative charge across the capsid at high pH ͓Fig. 8 ͑right panel͔͒ diminishes its stability due to Coulombic repulsion.…”

Section: The Outer Surfacementioning

confidence: 99%

An analytical approach to computing biomolecular electrostatic potential. II. Validation and applications

Gordon

Fenley

Onufriev

2008

The Journal of Chemical Physics

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An ability to efficiently compute the electrostatic potential produced by molecular charge distributions under realistic solvation conditions is essential for a variety of applications. Here, the simple closed-form analytical approximation to the Poisson equation rigorously derived in Part I for idealized spherical geometry is tested on realistic shapes. The effects of mobile ions are included at the Debye-Hückel level. The accuracy of the resulting closed-form expressions for electrostatic potential is assessed through comparisons with numerical Poisson-Boltzmann ͑NPB͒ reference solutions on a test set of 580 representative biomolecular structures under typical conditions of aqueous solvation. For each structure, the deviation from the reference is computed for a large number of test points placed near the dielectric boundary ͑molecular surface͒. The accuracy of the approximation, averaged over all test points in each structure, is within 0.6 kcal/ mol/ ͉e͉ϳkT per unit charge for all structures in the test set. For 91.5% of the individual test points, the deviation from the NPB potential is within 0.6 kcal/ mol/ ͉e͉. The deviations from the reference decrease with increasing distance from the dielectric boundary: The approximation is asymptotically exact far away from the source charges. Deviation of the overall shape of a structure from ideal spherical does not, by itself, appear to necessitate decreased accuracy of the approximation. The largest deviations from the NPB reference are found inside very deep and narrow indentations that occur on the dielectric boundaries of some structures. The dimensions of these pockets of locally highly negative curvature are comparable to the size of a water molecule; the applicability of a continuum dielectric models in these regions is discussed. The maximum deviations from the NPB are reduced substantially when the boundary is smoothed by using a larger probe radius ͑3 Å͒ to generate the molecular surface. A detailed accuracy analysis is presented for several proteins of various shapes, including lysozyme whose surface features a functionally relevant region of negative curvature. The proposed analytical model is computationally inexpensive; this strength of the approach is demonstrated by computing and analyzing the electrostatic potential generated by a full capsid of the tobacco ring spot virus at atomic resolution ͑500 000 atoms͒. An analysis of the electrostatic potential of the inner surface of the capsid reveals what might be a RNA binding pocket. These results are generated with the modest computational power of a desktop personal computer.

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Therapy of Virus-Infected Plants by Heat Treatment I. Some Properties of Tomato Aspermy Virus and Its Inactivation at 36°C

Cited by 7 publications

References 23 publications

High temperature inactivation of cucumber and alfalfa mosaic viruses in Nicotiana rustica cultures

High temperature inactivation of cucumber and alfalfa mosaic viruses in Nicotiana rustica cultures

Applied Plant Virology

An analytical approach to computing biomolecular electrostatic potential. II. Validation and applications

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