Thermal adaptation and heat shock response of <i>Tilapia</i> ovary cells

Chen, J. Don; Yew, F. H.; Li, Gloria C.

doi:10.1002/jcp.1041340204

Cited by 33 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results from these studies have shown that fish heat shock proteins can be induced by thermal stress (Airaksinen et al 1998;Chen et al 1988), anoxia (Ossum et al 2004) as well as heavy metal exposure (Heikkila et al 1982;Misra et al 1989). Of all the heat shock protein families, the hsp70 family bas been most widely studied as a biomarker of stress (Ryan and Hightower 1996) and in our previous studies on sea bream we have assessed the expression profile of hsp70 using in vivo approaches (Deane et al 2002).…”

Section: Discussionmentioning

confidence: 96%

Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies

Zhou

Deane

Woo

2003

Fish Physiol Biochem

View full text Add to dashboard Cite

A fibroblast cell line (BSF) derived from a caudal fin explant of black sea bream (Mylio macrocephalus) was developed. The optimum fetal bovine serum (FBS) concentration for fibroblast cell line growth was found to be 15-20% v/v FBS and the optimum temperature range for growth was found to be 26-30 • C. The fibroblast cells displayed a diverse distribution in chromosome number with two modal chromosome numbers of 48 and 54. Upon acute heat shock (+8 • C) the cells displayed a 4.1 fold increase in hsp70 and this elevation was not prolonged as hsp70 returned to near basal levels following a 6 h recovery period. The effect of the hsp70 inducer L-azetidine-2-carboxylic acid was tested and it was found that at a concentration of 10 mM this inducer caused a 2.3 fold increase in hsp70 levels. The sensitivity of the fibroblast cell line to heavy metal exposure was tested by treatment with Cu 2+ and it was found that hsp70 was significantly elevated in the presence of micromolar concentrations of Cu 2+ . The data from this study demonstrates that the established black sea bream fibroblast cell line could serve as a useful in vitro model for stress protein studies.

show abstract

Section: Discussionmentioning

confidence: 96%

Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies

Zhou

Deane

Woo

2003

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Data from many of these studies have shown that fish HSP expression is induced by a number of stressors, such as thermal shock (Airaksinen et al 1998, Chen et al 1988, Mosser & Bols 1988, heavy metal exposure (Heikkila et al 1982, Misra et al 1989, hypoxia (Airaksinen et al 1998) and exposure to infectious agents (Cho et al 1997). Although fewer experiments have been performed using whole fish, elevated levels of HSP70 in different tissues from Fundulus heteroclitus (Koban et al 1991), Pimephales promelas (Dyer et al 1991), Gillichthys mirabilis and Gillichthys seta (Dietz & Somero 1992) after thermal shock have been described.…”

Section: Introductionmentioning

confidence: 99%

Effects of GH, prolactin and cortisol on hepatic heat shock protein 70 expression in a marine teleost Sparus sarba

Deane

Kelly²,

Lo³

et al. 1999

Journal of Endocrinology

View full text Add to dashboard Cite

Heat shock protein 70 (HSP70) expression was assessed in hepatic tissue of a marine teleost Sparus sarba after exogenous hormone administration. Using a PCR-amplified, homologous HSP70 cDNA clone, as a probe in Northern analysis, we detected a 2·3 kb transcript which was elevated after exposure to a temperature 7 C above the ambient. For our studies on hormonal effects on HSP70 expression, groups of fish were administered recombinant bream GH (rbGH), ovine prolactin (oPRL) or cortisol daily over a 7-day period. Quantification of hepatic HSP70 transcript revealed that the administration of GH and PRL significantly reduced HSP70 mRNA abundance by 42% and 54% from saline-injected fish respectively. Also hepatic HSP70 levels were reduced by 76% and 64% as determined by immunoblotting after rbGH and oPRL treatment respectively. The administration of exogenous cortisol did not alter hepatic HSP70 mRNA or protein levels in S. sarba. The results obtained in this study are the first evidence for hormonal modulation of heat shock protein expression in fish. The significance of these results is discussed within the context of current knowledge on the roles of these hormones in teleostean stress response.

show abstract

“…Other researchers using Tilapia sp. ovary cells (Chen et al, 1988), chinook salmon embryonic cells (Heikkila et al,. 1982;Gedamu et al, 1983), rainbow trout hepatoma (Misra et al, 1989) and fibroblast cells (Mosser et al, 1986) have not only shown high and medium molecular weight hsp's (100-60 kD) but also low molecular weight hsp's (42-19 kD) upon hyperthermic exposures.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Efficacy of the Stress Protein Response as a Biochemical Water Quality Biomonitoring Method

Dyer¹,

Dickson²,

Zimmerman³

1990

View full text Add to dashboard Cite

To evaluate the potential use of the SPR as a biomonitoring tool, a stepwise plan was utilized that proceeded through various physical and chemical laboratory exposures and culminated with a field validation study. The goals of the laboratory exposures were threefold: 1) determine the time required for induction of the SPR; 2) determine the dose-responsiveness of the SPR; and 3) compare the increased syntheses and accumulations of stress proteins to classical toxicological endpoints (i.e. percent mortality, LC50, LC1, etc).Brain, gill, and striated muscle tissues of fathead minnows (Pimephales promelas) exposed to heat shock, sodium arsenite, sodium chromate, lindane, and diazinon elicited tissue and stressor specific translation patterns. Increased syntheses and accumulations of several proteins were found to be concentration dependent at environmentally relevant levels. All tissues under all exposure regimes elicited a 70 kD protein. This protein was determined to be indicative of general stress. Two proteins of 20 and 30 kD were only found in fish exposed to arsenite and chromate. Thus, these two proteins may serve as metal indicators.Gill of fathead minnows exposed in situ to a toxicity gradient in Soldier Creek, Midwest City, Oklahoma were found to accumulate stress protein 70 in a manner that reflected in stream fish species richness.The results of this research demonstrates that the stress protein response has potential as a rapid and environmentally relevant biomonitoring method for assessing both general and stressor specific perturbations in fish. Figure 3. A. A fluorogram of striated muscle protein from fathead minnows exposed to 34 0 C for 1-hour and then transferred to 25 0 C for 11 hours and sampled at 0, 1, 3, 7, and 11 hours post heat shock exposure. Molecular weight markers of 97, 66, 42, 31, 21, and 14 kD, respectively, are designated on the left. Arrows on the right denote heat shock proteins of 90, 78 and 70 kD. B. Median intensities of 90, 78, and 70 kD proteins in fluorograms from replicate samples (n = 3) of experiment described in A. Intensities were determined densito metrically and are presented as arbitrary units (see Materials and methods Unfortunately, their clinical use is lacking (Bouk, 1984). Clinical use implies that the method has been validated by extensive testing-both to identify the biological significance of the parameter and the limitations in applying the test to the species in question.Much of the early biomarker work involved measurements of blood and enzyme activity as well as biochemical composition of blood and tissues (Mazeud and Mazeud, 1981;Neff, 1985), methods primarily transplanted from human and veterinary medicine. Unfortunately, many of these parameters were not applicable across phyla and measured secondary effects, symptoms, of an underlying problem rather than highly conserved parameters intimately involved in the protection and/or defense of the cell. Thus, biomarkers that correspond to events that are coupled to cellular protection and defens...

show abstract

Thermal adaptation and heat shock response of Tilapia ovary cells

Cited by 33 publications

References 27 publications

Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies

Development of a black sea bream fibroblast cell line and its potential use as an in vitro model for stress protein studies

Effects of GH, prolactin and cortisol on hepatic heat shock protein 70 expression in a marine teleost Sparus sarba

Evaluation of the Efficacy of the Stress Protein Response as a Biochemical Water Quality Biomonitoring Method

Contact Info

Product

Resources

About