2018
DOI: 10.3390/s18061812
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Thermal Analysis of a Disposable, Instrument-Free DNA Amplification Lab-on-a-Chip Platform

Abstract: Novel second-generation rapid diagnostics based on nucleic acid amplification tests (NAAT) offer performance metrics on par with clinical laboratories in detecting infectious diseases at the point of care. The diagnostic assay is typically performed within a Lab-on-a-Chip (LoC) component with integrated temperature regulation. However, constraints on device dimensions, cost and power supply inherent with the device format apply to temperature regulation as well. Thermal analysis on simplified thermal models fo… Show more

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Cited by 8 publications
(5 citation statements)
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“…However, they can also be used to study electromagnetic or thermal effects. For instance, they can be used to analyze heat distribution under various operating conditions in microchannels where measurements would be difficult in situ [ 143 , 144 ]. Furthermore, they can be used to analyze electromagnetic fields and optimize electrode or magnet placement [ 100 , 145 ].…”
Section: Democratizing 3dp Droplet Microfluidicsmentioning
confidence: 99%
“…However, they can also be used to study electromagnetic or thermal effects. For instance, they can be used to analyze heat distribution under various operating conditions in microchannels where measurements would be difficult in situ [ 143 , 144 ]. Furthermore, they can be used to analyze electromagnetic fields and optimize electrode or magnet placement [ 100 , 145 ].…”
Section: Democratizing 3dp Droplet Microfluidicsmentioning
confidence: 99%
“…For measuring temperatures lower than 130 Celsius, the ubiquitously available analog temperature sensor TMP36 can be used [ 32 , 33 ]. For measurement of higher temperatures, up to 1000 Celsius, a suitable thermocouple and break-out board containing temperature sensing electronics can be selected [ 31 , 34 ]. For building of a thermocycler such as a polymerase chain reaction (PCR) machine, it is informative to look at the work of Chai, a company founded by Josh Perfetto and Jessie Ho at where they built up a PCR thermocycler based on an Arduino Uno.…”
Section: How To Get Startedmentioning
confidence: 99%
“…Self-regulating resistive heating elements are an interesting novel solution to integrated temperature control as they require no thermostat [21,22,23,24,25]. However, due to the open-loop nature of the PTCR (positive temperature coefficient of resistance) effect used for control, they require extensive optimization to the particular NAAT protocol and ambient temperature conditions they are used in [21]. Noncontact electrical heating solutions include induction and microwave heating [26], but are impractical for portable applications.…”
Section: Introductionmentioning
confidence: 99%
“…In previous works, we demonstrated the integration of commercially available electrical heating elements into isothermal LoC NAAT devices [46,47,48,49,50]. Furthermore, we demonstrated the application of a self-regulating PTCR polymer-resin heating element in a LoC NAAT device, including thermal analysis [21,51] and proof-of-concept LAMP tests [22]. Previously we focused exclusively on low-cost, integrated heating solutions for use specifically in single-use LoC devices and primarily on the LAMP assay.…”
Section: Introductionmentioning
confidence: 99%
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