Thermal Analysis of Infrared Irradiation-Assisted Nanosecond-Pulsed Tumor Ablation

Hornef, James; Edelblute, Chelsea; Schoenbach, K.H.; Heller, Richard; Guo, Siqi; Jiang, Chunqi

doi:10.1038/s41598-020-62017-8

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…When the application of moderate heating and electric field is combined, these effects are more pronounced and delivery is more efficient. Moderate heating is also advantageous in the context of irreversible elec troporation, where the applied heat sensitizes cancer cells to the applied electric field, thus making a more effective treatment [48,67,68].…”

Section: Discussionmentioning

confidence: 99%

Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin

2021

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Gene-based approaches for protein replacement therapies have the potential to reduce the number of administrations. Our previous work demonstrated that expression could be enhanced and/or the applied voltage reduced by preheating the tissue prior to pulse administration. In the current study, we utilized our 16-pin multi-electrode array (MEA) and incorporated nine optical fibers, connected to an infrared laser, between each set of four electrodes to heat the tissue to 43 °C. For proof of principle, a guinea pig model was used to test delivery of reporter genes. We observed that when the skin was preheated, it was possible to achieve the same expression levels as gene electrotransfer without preheating, but with a 23% reduction of applied voltage or a 50% reduction of pulse number. With respect to expression distribution, preheating allowed for delivery to the deep dermis and muscle. This suggested that this cutaneous delivery approach has the potential to achieve expression in the systemic circulation, thus this protocol was repeated using a plasmid encoding Human Factor IX. Elevated Factor IX serum protein levels were detected by ELISA up to 100 days post gene delivery. Further work will involve optimizing protein levels and scalability in an effort to reduce application frequency.

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Section: Discussionmentioning

confidence: 99%

Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin

2021

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“…Thermal properties of biological targets are evaluated according to the treatment area, penetration depth, and heat distribution due to laser irradiation with nsPEF. The system provides a detailed thermal analysis of pig skin with infrared laser irradiation and is validated in a tumor-bearing mouse model [137].…”

Section: Thermal Therapymentioning

confidence: 99%

Fiber-Optic Biosensors for Cancer Theranostics: From in Vitro to in Vivo

Jin,

Xu,

Cao

et al. 2024

Photonic Sens

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Cancer has been one of the most serious diseases, resulting in more than 10 million deaths every year. Fiber-optic sensors have great potential for diagnosing and treating cancer due to their flexibility, precise positioning, real-time monitoring, and minimally invasive characteristics. Compared to traditional central laboratory examination, fiber-optic biosensors can provide high sensitivity, miniaturization, and versatility, which feature the point-of-care diagnostic capability. Herein, we focus on recent advances in fiber-optic biosensors for cancer theranostics. It is primarily concerned with advancements in the design of various fiber sensing approaches, fiber cancer sensing, and therapy sensors. With fiber-optic biosensors, cancer marker detection, cancerous cell differentiation, ex vivo tumor model validation, and in vivo tumor detection can be achieved. And the medical fiber also can be used to provide photothermal therapy, photodynamic therapy, and combination therapy for solid tumors. Additionally, cancer sensing and therapy can be integrated into the fiber, which demonstrates the multiplexing capabilities of fiber-optic biosensors. Lastly, we systematically summarize the fiber biosensor applications from in vitro to in vivo, and conclude with the challenges in development and prospects.

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“…Instead, it induces apoptosis of tumour cells and inhibits tumour angiogenesis. As of today, nsPEF has been widely used to treat a great number of cancers, including malignant melanoma (Chen et al, 2010;Rossi et al, 2019;Zhang et al, 2019), skin basal cell carcinoma (Yang et al, 2011), lung squamous cell cancer (Hornef et al, 2020), as well as skin squamous cell carcinoma (Yin et al, 2012). Previous studies have reported the treatment of HCC with nsPEF in animal models since nsPEF does not cause direct Joel heating but induces complete cancer cell death (Yin et al, 2014;Xu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Multi-Omics Analysis Reveals Disturbance of Nanosecond Pulsed Electric Field in the Serum Metabolic Spectrum and Gut Microbiota

Dong

Wang

et al. 2021

Front. Microbiol.

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Nanosecond pulsed electric field (nsPEF) is a novel ablation technique that is based on high-intensity electric voltage to achieve tumour-killing effect in the target region, and increasingly considered for treating tumours of the liver, kidneys and other organs with rich blood supply. This study aims to observe effect of nsPFE treatment on serum metabolites and gut microbiota. The serum and faecal specimens of the pigs were collected pre- and post-treatment. The gut microbiota of pigs was sequenced by Illumina Miseq platform for analysing the diversity and alterations of gut microbiota. Liquid chromatography-mass spectrometry (LC-MS)-based metabonomic analysis and Pearson coefficient method were also used to construct the interaction system of different metabolites, metabolic pathways and flora. A total of 1,477 differential metabolites from the serum were identified by four cross-comparisons of different post-operative groups with the control group. In addition, an average of 636 OTUs per sample was detected. Correlation analysis also revealed the strong correlation between intestinal bacteria and differential metabolites. The nsPEF ablation of the liver results in a degree of liver damage that affects various metabolic pathways, mainly lipid metabolism, as well as gut microbiota. In conclusion, our study provided a good point for the safety and feasibility of applying nsPEF on liver through the integrated analysis of metabolomics and microbiomes, which is beneficial for the improvement of nsPEF in clinical use.

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Thermal Analysis of Infrared Irradiation-Assisted Nanosecond-Pulsed Tumor Ablation

Cited by 9 publications

References 30 publications

Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin

Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin

Fiber-Optic Biosensors for Cancer Theranostics: From in Vitro to in Vivo

Multi-Omics Analysis Reveals Disturbance of Nanosecond Pulsed Electric Field in the Serum Metabolic Spectrum and Gut Microbiota

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