Thermal and hydrolytic degradation of electrospun fish gelatin membranes

Correia, Daniela M.; Padrão, Jorge; Rodrigues, L. R.; Dourado, Fernando; Lanceros‐Méndez, S.; Sencadas, Vítor

doi:10.1016/j.polymertesting.2013.05.004

Cited by 71 publications

(57 citation statements)

References 27 publications

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“…Poly(vinyl alcohol) (PVA) gels are often applied as cartilage substitutes as they may mimic the elastic modulus of cartilage 20,21 , as they offer the possibility of formation of physical cross-links during freezing/thawing cycles without the need of toxic monomers used typically in chemically cross-linked gels 22 such as gelatin 23 or chitosan 24 . During exposure to cold temperatures promoting water freezing, phase separation leads to regions of high PVA concentration, hydrogen bonding and crystallite formation due to the higher packing of the PVA chains 25 .…”

Section: Introductionmentioning

confidence: 99%

In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

et al. 2014

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In vitro mechanical fatigue behavior of poly-ɛ-caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel AbstractPolymeric scaffolds used in regenerative therapies are implanted in the damaged tissue and submitted to repeated loading cycles. In the case of articular cartilage engineering, an implanted scaffold is typically subjected to long-term dynamic compression. The evolution of the mechanical properties of the scaffold during bioresorption has been deeply studied in the past, but the possibility of failure due to mechanical fatigue has not been properly addressed. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. In this work fatigue studies of polycaprolactone scaffolds were carried by subjecting the scaffold to repeated compression cycles in conditions simulating the scaffold implanted in the articular cartilage. The behavior of the polycaprolactone sponge with the pores filled with a poly(vinyl alcohol) gel simulating the new formed tissue within the pores was compared with that of the material immersed in water. Results were analyzed with Morrow's criteria for failure and accurate fittings are obtained just up to 200 loading cycles. It is also shown that the presence of poly(vinyl alcohol) increases the elastic modulus of the scaffolds, the effect being more pronounced with increasing the number of freeze/thawing cycles. AbstractPolymeric scaffolds used in regenerative therapies are implanted in the damaged tissue and submitted to repeated loading cycles. In the case of articular cartilage engineering, an implanted scaffold is typically subjected to long term dynamic compression. The evolution of the mechanical properties of the scaffold during bioresorption has been deeply studied in the past, but the possibility of failure due to mechanical fatigue has not been properly addressed.Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. In this work fatigue studies of polycaprolactone scaffolds were carried by subjecting the scaffold to repeated compression cycles in conditions simulating the scaffold implanted in the articular cartilage. The behaviour of the polycaprolactone sponge with the pores filled with a poly(vinyl alcohol) gel simulating the new formed tissue within the pores was compared with that of the material immersed in water. Results were analyzed with Morrow's criteria for failure 2 and accurate fittings are obtained just up to 200 loading cycles. It is also shown that the presence of poly(vinyl alcohol) increases the elastic modulus of the scaffolds, the effect being more pronounced with increasing the number of freeze/thawing cycles.

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Section: Introductionmentioning

confidence: 99%

In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

et al. 2014

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“…Moreover, cross-linking is typically associated to a colour change from white to pale yellow [25]. These colour changes have been attributed to the formation of aldimine linkages (-CH=N-) between the free amino acids of lysine or hydroxylysine amino acids residues of the protein and the aldehyde groups of GA [41,51]. (assigned to N-H bending vibration) [52].…”

Section: Resultsmentioning

confidence: 99%

“…Fish gelatine (FG) and formic acid (FA) were purchased from SigmaAldrich and N,N-dimethylformamide (DMF) was acquired from Merck. The polymer solution was prepared as described elsewhere [41]. Briefly, fish gelatine was dissolved in a mixture of FA and DMF (4:6 v/v) at 50 °C to achieve a final gelatine concentration of 30 wt%.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes

Padrão

Machado

Casal

et al. 2015

International Journal of Biological Macromolecules

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The increase of antibiotic resistant microorganisms urged the development and synthesis of novel antimicrobial biomaterials to be employed in a broad range of applications, ranging from food packaging to medical devices. This work describes the production and characterization of a protein-based electrospun fibrous membranes bearing antimicrobial properties. Its composition is exclusively comprised of proteins, with fish gelatine as structural matrix and bovine lactoferrin (bLF) as the active antimicrobial agent. The bLF bactericidal effect was determined against clinical isolates of Escherichia coli and Staphylococcus aureus through microdilution assays. Two distinctive methods were used to incorporate bLF into the fish gelatine nanofibres: (i) as a filler in the electrospinning formulation with concentrations of 2, 5 and 10 (wt%), and cross-linked with glutaraldehyde vapour, in order to achieve stability in aqueous solution; and (ii) through adsorption in a solution with 40mgmL -1 bLF. Fourier transform infrared spectroscopy analysis showed that the structure of both proteins remained intact through the electrospinning blending and cross-linking procedure. Remarkable antibacterial properties were obtained with membranes containing 5% and 10% bLF with a bacterial reduction of approximately 90% and 100%, respectively. and 10 (%wt), and cross-linked with glutaraldehyde vapour, in order to achieve stability in aqueous solution; and ii) through adsorption in a solution with 40 mg mL -1 bLF. Fourier transform infrared spectroscopy analysis showed that the structure of both proteins remained intact through the electrospinning blending and cross-linking procedure. Remarkable antibacterial properties were obtained with membranes containing 5% and 10% bLF with a bacterial reduction of approximately 90% and 100%, respectively.

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“…These color changes (along with the shrinkage effect) have been attributed to the formation of aldimine linkages (-CH=N-) between the free amino acids of lysine or hydroxylysine amino acids residues of the protein, and the aldehyde groups of GA [22,27]. Cross-linked gelatin fiber mats exposed for 24 h to GA vapor were immersed in PBS at room temperature, and the swelling behavior was measured over time (Figure 3a).…”

Section: Resultsmentioning

confidence: 99%

“…Finally, the modified membranes were evaluated regarding their viability for biological applications by evaluating cell adhesion and proliferation of mouse embryo fibroblast 3T3 cells (MC-3T3-E1) in vitro. [22]. A film of gelatin was processed from the above-mentioned solution by adding 1 % GA to the solution and before spreading into a Teflon dish.…”

Section: Introductionmentioning

confidence: 99%

Modifying Fish Gelatin Electrospun Membranes for Biomedical Applications: Cross-Linking and Swelling Behavior

Padrão¹,

Silva²,

Rodrigues³

et al. 2014

Soft Materials

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Development of suitable membranes is a fundamental requisite for tissue and biomedical engineering applications. This work presents fish gelatin random and aligned electrospun membranes cross-linked with glutaraldehyde (GA). It was observed that the fiber average diameter and the morphology is not influenced by the GA exposure time and presents fibers with an average diameter around 250 nm. Moreover, when the gelatin mats are immersed in a phosphate buffered saline solution (PBS), they can retain as much as 12 times its initial weight of solution almost instantaneously, but the material microstructure of the fiber mats changes from the characteristic fibrous to an almost spherical porous structure. Cross-linked gelatin electrospun fiber mats and films showed a water vapor permeability of 1.37 ± 0.02 and 0.13 ± 0.10 (g.mm)/(m 2 .h.kPa), respectively. Finally, the processing technique and cross-linking process does not inhibit MC-3T3-E1 cell adhesion. Preliminary cell culture results showed good cell adhesion and proliferation in the cross-linked random and aligned gelatin fiber mats.

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Thermal and hydrolytic degradation of electrospun fish gelatin membranes

Cited by 71 publications

References 27 publications

In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel

Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes

Modifying Fish Gelatin Electrospun Membranes for Biomedical Applications: Cross-Linking and Swelling Behavior

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