Thermal deactivation of Pseudomonas aeruginosa biofilms

O’Toole, Ann

doi:10.17077/etd.78yn0isg

Cited by 9 publications

(22 citation statements)

References 33 publications

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“…The drip flow reactor has been shown to be a good model for growing large, bacterial dense, biofilms. 120 This was in comparison to other growth means, such as the shaker plate biofilm, explored more in Chapter 5, which had lower density biofilms than the drip flow reactor-grown biofilms. It was discovered early on that in order to quantify the amount of bacterial death observed a very high number of bacteria was required to accurately report the reduction.…”

Section: These Experiments Provide a Better Understanding Of Biofilmsmentioning

confidence: 93%

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The synergistic effects of orthogonal biofilm mitigation strategies

Ricker¹

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Section: These Experiments Provide a Better Understanding Of Biofilmsmentioning

confidence: 93%

“…Biofilms cultured in drip flow reactors were thicker (50-150 µm) and more carpetlike, as shown in Figure 3.2, than biofilms grown in shaker plates. 121…”

Section: Drip Flow Reactor Biofilmsmentioning

confidence: 99%

The synergistic effects of orthogonal biofilm mitigation strategies

Ricker¹

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“…To investigate the effect heat treatment has on biofilms, P. aeruginosa biofilms have been thermally shocked at medically accessible times and temperatures ranging from 1 min to 30 min and from 37 °C to 80 °C. 27 In that study, the thermal load was provided by a temperature controlled water bath and demonstrated up to six orders of magnitude reduction in colony forming units (CFUs) per cm 2 2. Thermal deactivation of biofilms using a water bath heat shock method.…”

Section: Thermal Deactivationmentioning

confidence: 99%

“…If (time.eq.1.or.mod(time,int((ttotal/60/delt))).eq.0) Then Do k=z2+1,z3 qz(k-z2)=(3.0*T(imax/2,y2+1,k)-4.0* T(imax/2,y2+2,k) & +T(imax/2,y2+3,k))*kcon(imax/2,y2+1,k)/2.0/dely/10000.0 End Do write(4,40)time*delt,qz(1), qz(2), qz(3), qz(4), qz (5), & qz (6), qz (7), qz (8), qz(9), qz(10), & qz (11), qz (12), qz (13), qz (14),qz (15), & qz (16), qz (17), qz (18), qz (19),qz (20), & qz (21), qz (22), qz (23), qz(24),qz (25), & qz (26), qz (27), qz (28), qz (29),qz (30), & qz (31), qz (32), qz (33), qz (34),qz (35), & qz (36), qz (37), qz (38), qz 39 Do k=5,kmax-4,2 rhoustar(imax-1,j,k)=rhoustar(imax-3,j,k) End Do End Do Do j=3,jmax-2,2 Do k=3,kmax-2,2 rhovstar(imax,j,k)=rhovstar(imax-2,j,k) End Do End Do Do j=4,jmax-3,2 Do k=4,kmax-3,2 rhowstar(imax-1,j,k)=rhowstar(imax-3,j,k) End Do End Do !using rhoustar, rhovstar, rhowstar, solve for the corrected pressure ! (denoted as pp)using the pressure correction formula which is solved !using the successive overrelaxation subroutine (sor) !prepare e matrix for sor subroutine !e can be thought of as a mass source term and represents how far the !continuity equation deviates from the converged answer.…”

Section: Expanding the Computational Modelmentioning

confidence: 99%

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Implementation and modeling of in situ magnetic hyperthermia

Coffel¹

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Millions of medical devices are implanted in patients annually; of these, hundreds of thousands become infected on difficult-to-treat implant surfaces such as artificial hip joints, bone pins, catheters, and cardiac devices. The bacteria that cause these infections are highly resistant to antibiotics and the patient's immune system. Consequently, the majority of infected devices are surgically removed to treat the infection with a subsequent additional surgery to replace the implant. The complications resulting from medical device infections cost patients and hospitals upwards of $5 billion annually, in addition to costing the patient a diminished quality of life. This work develops a material that can be used to remotely treat (i.e. without having the patient go under the knife) implanted medical device associated infections with heat energy delivered from a magnetic coating. By increasing the temperature of the bacteria using wirelessly delivered energy, a potential treatment is developed that does not rely on any drugs or chemicals (to which bacteria can develop resistance) or invasive surgery techniques. Wirelessly heated coatings were developed that can heat bacteria grown on their surface to temperatures as hot as 175 °F. These coatings are shown to kill bacteria that cause medical device infections to non-quantifiable levels. Additionally, by modeling the heat transfer from this coating in the human body, we can predict how much power is needed by the magnetic coating and how to deliver the treatment safely without causing severe thermal damage to the tissue and organs surrounding the medical device in the patient. v TABLE OF CONTENTS LIST OF TABLES .

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Remote control of biofouling by heating PDMS/MnZn ferrite nanocomposites with an alternating magnetic field

Panigrahi

Sharma

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The accumulation of unwanted microorganisms on wetted surfaces, leading to surface damage and contamination, is a common and significant global issue. RESULTS: Herein, we report a novel technique where the growth of microorganisms can be readily controlled by coating the surfaces with a polydimethylsiloxane (PDMS)/Mn 0.8 Zn 0.2 Fe 2 O 4 (manganese-zinc ferrite) nanocomposite followed by applying alternating magnetic field (AMF). The PDMS/MnZn ferrite nanocomposite is light weight and thermally stable (up to ∼ 330 ∘ C) that can form a flexible coating. PDMS also provides hydrophobicity, which is further enhanced by the addition of Mn and Zn. The improved hydrophobicity makes the coated surface less susceptible to biofilm formation. When external AMF was applied to nanocomposites containing various MnZn ferrite nanoparticle loads of 10%, 20% and 30%, the temperature of the surface of nanocomposites reached to 80, 120 and 160 ∘ C, respectively. Successful biofilm deactivation was achieved by heating the nanocomposites via AMF application, as shown in the biofilm test where up to ∼ 70% of the Pseudomonas aeruginosa PAO1 biofilm cells were killed when the AMF was applied for 20 min to the nanocomposites containing 30% nanoparticles. CONCLUSION: Coating the surface with PDMS/MnZn ferrite nanocomposites followed by applying external AMF can be an effective way to remove biofilm remotely in a wide range of applications. Evaluation of iron-cobalt/ferrite core-shell nanoparticles for cancer thermotherapy. J Appl Phys 103:07A307 (2008). 25 McNerny KL, Kim Y, Laughlin DE and McHenry ME, Chemical synthesis of monodisperse -Fe-Ni magnetic nanoparticles with tunable J Chem Technol Biotechnol 2019; 94: 2713-2720

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Thermal deactivation of Pseudomonas aeruginosa biofilms

Cited by 9 publications

References 33 publications

The synergistic effects of orthogonal biofilm mitigation strategies

The synergistic effects of orthogonal biofilm mitigation strategies

Implementation and modeling of in situ magnetic hyperthermia

Remote control of biofouling by heating PDMS/MnZn ferrite nanocomposites with an alternating magnetic field

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