Factors influencing the occurrence of gelation and clarification of frozen citrus concentrates and means of preventing these defects have been studied by a number of investigators (4,5,16,33,34,37,38). Rouse (37) reported that gelation was caused by pectinesterase (PE ) demethylating pectin t o form low-methoxyl pectin which, in the presence of a sufficient concentration of polyvalent cations, reacted to form a pectinate gel. Wenzel, Moore, Rouse, and Atkins (40) pointed out that the pectin content, PE activity, and the concentration of polyvalent cations in citrus concentrates were major factors associated with gel formation. They demonstrated that gelation and clarification could be prevented by storing the product a t o r below -18"C.(OoF.) or by thermal inactivation of PE in the unconcentrated juice. Moore, Huggart, and Hill (33) showed that unpasteurized, concentrated grapefruit and orange juice stored €or 6 months at -7", Oo, and 4.4"C.(2Oo, 32", and 40°F.) gelled o r clarified.According to Phaff and Joslyn (35) the action of P E was first described by Fri5my in 1840 who observed that when juice from young carrots was. mixed with pectin solution, gel formation invariably took place. Baker (6) and Kertesz (26) have summarized the results of several investigators who have studied enzyme deesterification of pectin, factors influencing the characteristics of low methoxyl-metallic pectinate gels, and the uses t G which low methoxyl gels may be put.Cruess (15) was among the first to report gelation and clarification of citrus juices. While working with orange juice he observed a sample that gelled, later the gel coagulated and settled out carrying the suspended particles of fruit with it, leaving the juice clear. He also demonstrated that pasteurization would prevent settling out of the suspended particles. Joslyn and Marsh (20) and Joslyn and Sedky (22) demonstrated that pectic enzymes were responsible for clarification. Loeffler (27) reported that PE enzymic changes occur rapidly in orange juice. Kertesz (24,25,26) has. studied the clarifying action of tomato P E thoroughly.Clarification of fruit juices may also occur as the result of depolymerizing enzymes. McColloch and Kertesz (3f) found an enzyme in tomatoes, which they called tomato pectic acid depolymerase (PD), which under certain conditions could cause clarification of tomato juice o r reduce the viscosity of tomato pulp (28,32,32). Pectin-polygalacturonase (PG) of "Journal Series Paper No. 4, College Agricultural Experiment Station, University of Georgia, Athens, .Georgia bA portion of a thesis submitted by Dan E. P r a t t to the Graduate Faculty, University of Georgia, June 1, 1952, in partial fulfillment of the requirements for the degree, Master of Science in Agriculture.152