“…The threedimensional plots (surface plots) of the fitted polynomial were generated to visualise the relationships between the responses and the independent variables using the statistical software. The decrease in luminosity during the storage time in fresh orange juices is attributed to the decantation of unstable particles in juice during the storage time (Genovese, Elustondo, & Lozano, 1997) and pigment break down, mainly carotenoids and lycopene (Shi & Maguer, 2000;Krebbers et al, 2003;Meléndez-Martínez, Vicario, & Heredi , a 2003;Vikram, Ramesh, & Prapulla, 2005); however, juices treated with chitosan did not show this behaviour (Fig. 4).…”