Thermal Inkjet Technology for the Microdeposition of Biological Molecules as a Viable Route for the Realization of Biosensors

Setti, Leonardo; Piana, Carlo; Bonazzi, Stefania; Ballarin, Barbara; Frascaro, D.; Fraleoni‐Morgera, Alessandro; Giuliani, S.

doi:10.1081/al-120037587

Cited by 57 publications

(49 citation statements)

References 15 publications

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“…However, previous work carried out on inkjet printing of proteins has reported that damage to the proteins is encountered upon printing, demonstrated by a drop in the retained activity or fluorescence of the protein [3][4][5][6]. Therefore, this work has a two fold purpose: to clarify what damage is occurring, if any, upon printing and to rectify this; and to produce a working solution and method for the deposition of the solutions or analytes onto the electrode using inkjet drop on demand printing technology.…”

Section: Introductionmentioning

confidence: 99%

Inkjet Printing of Enzymes for Glucose Sensors

2009

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Drop on demand inkjet printing is a potential method for depositing enzymes onto electrodes for sensor applications. This technology offers drop sizes in the region of picolitres and allows a production rate up to 200 mm/s. This enables not only a more rapid method of device prototyping but also a method for possible miniaturization of the sensors themselves. However, previous work [1] has indicated that inkjet printing may cause a drop in the retained activity of the enzyme.Here we assess the criticality of this drop in activity and how it may have been influenced by changes to the protein structure during printing. The enzyme used is glucose oxidase and the test methods include; protein analysis, in the form of analytical ultra-centrifugation and circular dichroism, scanning electron microscopy, atomic force microscopy and phase contrast microscopy, to analyse the surface topology of the electrodes and contact angle analysis, to assess the degree of spreading and the interactions between the drops and the electrode surface.With glucose oxidase there is no change in the conformation, structure or hydrodynamic radius of the protein after printing. The analysis of the electrode surface shows a relatively smooth surface that is made up of individual graphite flakes laid down by a screen printing method. When contact angle and spreading analysis is carried out it demonstrates reliability in the printing process as well as a drop in the sessile volume of the drop in conjunction with a growth in the base diameter of the drop as expected. It also demonstrates a fairly quick rate of evaporation of the drop. Upon the addition of surfactants to the solution the spreading is seen to be more extensive in relation to the surfactant concentration, although some initial reduction in experienced at low concentrations which may be due to the absorption into the carbon surface.

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Section: Introductionmentioning

confidence: 99%

Inkjet Printing of Enzymes for Glucose Sensors

2009

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“…Setti et al (2004) used a prototype model of thermal inkjet to dispense b-glactosidase (GAL) enzyme from Aspergillus oryzae. The enzyme was in phosphate buffer.…”

Section: Piezeoelectric and Thermal Inkjet Technologies In Biomoleculmentioning

confidence: 99%

Printing technologies for biomolecule and cell-based applications

Ihalainen

Määttänen

Sandler

2015

International Journal of Pharmaceutics

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“…Hence, the simpler process of pre-mixing (with pH adjustment to 7.0) was used for all further Table 1 Summary of the conditions used for the biosensor fabrication methods with their respective catalytic signals and signal-to-background (S/B) ratios for catalytic reduction of investigations. This method, as well as yielding the highest catalytic signals, also has the advantage of being the simplest method for incorporating protein, and could be developed further with techniques such as ink-jet printing [20,21]. Using the simultaneous casting method (with pH adjustment) for biosensor fabrication, the concentration of HRP was varied over the range 0-50 mg ml À1 in order to determine the optimum working concentration.…”

Section: Preparation Of Nanopani/dbsa Biosensorsmentioning

confidence: 99%

“…This was shown to be an effective biosensor format. The signal-to-background (S/B) was comparable to the biosensor developed using electrodeposited nanoparticles [19] but has the added advantage of ease of fabrication; a method that would lend itself to techniques such as ink-jet printing [20,21] for mass production of biosensors.…”

Section: Introductionmentioning

confidence: 99%

Novel biosensor fabrication methodology based on processable conducting polyaniline nanoparticles

Morrin

Wilbeer

Ngamna

et al. 2005

Electrochemistry Communications

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This work investigates polyaniline (PANI) nanoparticles, (synthesised using dodecylbenzenesulphonic acid (DBSA) as a dopant), as a novel, highly processable, non-diffusional mediating species in an enzyme biosensing application. These nanoparticles are readily dispersed in aqueous media which helps overcome some of the processability issues traditionally associated with polyaniline. Modification of screen-printed electrodes was readily achieved with these aqueous nanoparticle dispersions, where the nanoparticles were simply cast by a drop-coating method onto the surface. After suitable pH adjustment, it was shown that horseradish peroxidase (HRP) enzyme could be added to the dispersion, and cast simultaneously with the conducting polyaniline. This effective fabrication method involves no electrochemical steps, and as such is easily amenable to mass production. The feasibility of casting enzyme with polyaniline nanoparticles is demonstrated in this short communication. More accurate deposition of protein-containing inks onto screen-printed carbon working electrodes could in the future transfer the drop-coating protocol from manual deposition to largescale production by mechanical methods such as ink-jet printing.

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Thermal Inkjet Technology for the Microdeposition of Biological Molecules as a Viable Route for the Realization of Biosensors

Cited by 57 publications

References 15 publications

Inkjet Printing of Enzymes for Glucose Sensors

Inkjet Printing of Enzymes for Glucose Sensors

Printing technologies for biomolecule and cell-based applications

Novel biosensor fabrication methodology based on processable conducting polyaniline nanoparticles

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