Thermal manipulation during broiler chicken embryogenesis: Effect on mRNA expressions of Hsp108, Hsp70, Hsp47 and Hsf-3 during subsequent post-hatch thermal challenge

Al-Zghoul, Mohammad-Borhan; Dalab, Abd Elhafeed S.; Yahya, Imadeldin; Althnaian, Thnaian A.; Al-Ramadan, Saeed Y.; Ali, Abdelhadi M.; Albokhadaim, Ibrahim; El-Bahr, Sabry M.; Busadah, Khalid Ahmed Al; Hannon, Kevin

doi:10.1016/j.rvsc.2015.10.015

Cited by 26 publications

(35 citation statements)

References 44 publications

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“…Moreover, in post-hatch day 14; TM1 group was significantly higher in body live weight, skinned carcass, breast and thigh muscle, gut, liver, feather and skin weights when compared to the control. These results may indicate that thermal manipulation at early-term broiler embryogenesis has the bestpositive influence on hatchabilty and body performance.In contrast, other previous studies on the effects of thermal manipulation during early embryonic day 7 up to 16 and late during embryonic day 16 to18 at 38.8 , 39.5°Cor 41 for 24h on hatchability and body performance showed significant reduction in hatching rate or only slightly reduced it (Willemsen et al, 2010;Al-Zghoul et al, 2013;Al-Zghoul et al, 2015;Piestun et al, 2015;Al-Zghoul, 2018;Shanmugasundaram et al, 2019;Decuypere & Michels, 1992). On the other hand, our findings in TM3 and TM4 treatments of thermal manipulation at late-or long-term showed the same adverse effect on hatchability rate, chick quality and growth when compared with the control group.…”

Section: Discussioncontrasting

confidence: 64%

Morphological Investigations of the Effect of Thermal Manipulation During Embryogenesis on Body Performance and Structure of Pectoral and Thigh Muscle of Ross Broiler Chicken

Dalab

Ali

2019

Braz. J. Poult. Sci.

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The aim of this study was to determine the optimum timing of thermal manipulation during early, mid, late and long lasting embryogenesis that may result in improvement of body performance and myofiber development (fiber diameter and thickness of perimysium) of pectoral and thigh muscles by a comparative morphometrical investigation on post-hatch day 35. 1440 fertile Ross broiler eggs were divided randomly and equally into 5 treatment groups: control (no TM), TM1, TM2 TM3 and TM4 were thermally subjected to 39ºC for 18h with 65% relative humidity daily during embryonic days ED 7-11, ED 11-15, ED 15-18 and ED 7-18 respectively. Out of TM conditions that were investigated, TM1 resulted in a significant improvement of hatchability rate (95.14%) and body performance when compared to the control. On post-hatch day 35, there were significant differences in BW, skinned carcass, breast, thigh and gut weight for all groups when compared to each other with a higher significant in TM1 and TM2 when compared to those of the control. FCR in this study was lower in TM groups when compared to the control group. In TM3 and TM4 the chicks show a significantly lower value of body performance when compared with those of the control group, TM1 and TM2.The histological evaluation of Pectoral muscle revealed myofibers of different diameter (38.39-40.27 µm) and perimysial thickening (17-30.5 µm), while myofibers diameter of thigh muscle ranged between (29-32.9 µm) and the perimysial thickening ranged between (14.36-22.15 µm). Thus, these results indicate that TM duringED7-11 improved muscle growth and body performance and this finding may be applied by commercial breeders to produce more enhanced broiler chickens.

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Section: Discussioncontrasting

confidence: 64%

Morphological Investigations of the Effect of Thermal Manipulation During Embryogenesis on Body Performance and Structure of Pectoral and Thigh Muscle of Ross Broiler Chicken

Dalab

Ali

2019

Braz. J. Poult. Sci.

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“…In the present study, during CS, hepatic HSF3 expression was lower in TM chicks, but splenic expression did not differ between TM and control chicks. Previously, TM was found to enhance HSF3 expression during HS in Cobb chicks, but it did enhance basal HSF3 expression in TM chicks compared to controls under normal conditions [34]. In other studies, TM was found to increase HSF3 expression in Cobb and Hubbard chicks during post-hatch HS [13,35].…”

Section: Discussionmentioning

confidence: 87%

“…Previous reports have shown that, during HS, Hsp70 expression was found to be lower in a commercial broiler breed (Cobb) compared to local ones (Caneluda and Peloco), and this high expression level was associated with resistance to elevated temperatures in the local breeds [12]. Moreover, Cobb and Hubbard chicks subjected to TM were found to express higher cardiac, hepatic, muscular and splenic levels of Hsp70 during post-hatch heat challenge [13,34,35,42]. The differences between Hsp70 expression in control and TM chicks may be attributed to modulated DNA methylation of the Hsp70 promoter [55].…”

Section: Discussionmentioning

confidence: 92%

“…Previous studies used similar TM treatment parameters, including incubation at 39 • C for 18 h/day from ED 7-11, at 38.5 • C from ED 16 to 18 and at 39 • C for 18 h/day from ED 12 to 18, and these parameters were shown to significantly enhance BW as well as upregulate expression of muscle marker genes [27,[31][32][33]. The duration of TM was reported to affect thermotolerance acquisition in broilers, and different TM treatments led to differences in physiological parameters as well as varied mRNA expression profiles of heat shock genes [34][35][36][37].…”

Section: Discussionmentioning

confidence: 98%

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HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Tarkhan

Saleh

Al‐Zghoul

2020

Veterinary Sciences

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Decades of selective breeding for commercial purposes have rendered the broiler chicken (Gallus gallus domesticus) highly susceptible to heat and cold stress. A multitude of studies have documented the effects of thermal manipulation (TM) on broiler thermotolerance during periods of post-hatch heat stress, but very few have focused on the effect of TM on a broiler’s ability to withstand cold stress. Therefore, the primary objective of the current study is to determine the effects of TM on the acquisition of thermotolerance in broilers via their expression of the stress-associated 70 kilodalton heat shock protein (Hsp70) gene and heat shock factor 3 (HSF3) gene. Briefly, Hubbard broiler embryos were subject to TM by increasing the incubation temperature to 39 °C and 65% relative humidity (RH) for 18 h daily, from embryonic days (ED) 10 to 18. Broilers were then exposed to cold stress by decreasing the room temperature to 16 °C during post-hatch days 32 to 37. After thermal challenge, broilers were euthanized and hepatic and splenic tissues were collected. Our results showed that TM decreased the hatchability rate and body temperature but improved the body weight gain. TM generally decreased the hepatic expression but did not change the splenic expression of HSF3 during cold stress. In contrast, both hepatic and splenic Hsp70 expression decreased during cold stress. The results of the present study may suggest that TM significantly affects a broiler’s genetic response to cold stress.

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“…Several studies have verified only the influence of different thermal stress intensities, without varying the duration of the thermal stress (Al-Zghoul et al, 2015;Cândido et al, 2016: Zhang et al, 2016. However, analyzing the intensity and duration together makes it possible to investigate possible occurrences of adaptation of the bird to the stressful environment, depending on the exposure time, or to verify how stressor intensity can aggravate productivity losses due to longer or shorter exposure times.…”

Section: Introductionmentioning

confidence: 99%

Artificial Neural Networks for Prediction of Physiological and Productive Variables of Broilers

et al. 2020

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Due to a number of factors involving the thermal environment of a broiler cutting installation and its interaction with the physiological and productive responses of birds, artificial intelligence has been shown to be an interesting methodology to assist in the decision-making process. For this reason, the main aim of this work was to develop an artificial neural network (ANN) to predict feed conversion (FC), water consumption (Cwater), and cloacal temperature (tclo) of broilers submitted to different air dry-bulb temperatures (24, 27, 30, and 33ºC) and durations (1, 2, 3, and 4 days) of thermal stress in the second week of the production cycle. Relative humidity and wind speed were fixed at 60% and 0.2 ms -1 , respectively. The experimental data were used for the development of an ANN with supervised training using the Levenberg-Marquardt backpropagation algorithm. The ANN consisted of three input layers one hidden, and three output with sigmoidal tangent transfer functions with values between -1 and 1. The developed ANN has adequate predictive capacity, with coefficients of determination (R 2 ) for tclo, FC, and Cwater of 0.79, 0.87, and 0.97, respectively. In this way, the proposed ANN can be used as a support for decision-making to trigger poultry heating systems for broiler breeding.

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Thermal manipulation during broiler chicken embryogenesis: Effect on mRNA expressions of Hsp108, Hsp70, Hsp47 and Hsf-3 during subsequent post-hatch thermal challenge

Cited by 26 publications

References 44 publications

Morphological Investigations of the Effect of Thermal Manipulation During Embryogenesis on Body Performance and Structure of Pectoral and Thigh Muscle of Ross Broiler Chicken

Morphological Investigations of the Effect of Thermal Manipulation During Embryogenesis on Body Performance and Structure of Pectoral and Thigh Muscle of Ross Broiler Chicken

HSF3 and Hsp70 Expression during Post-Hatch Cold Stress in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Artificial Neural Networks for Prediction of Physiological and Productive Variables of Broilers

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