Thermal Melting Curves of tRNAPhe from Yeast Lacking Different Numbers of Nucleotides from the 3'-End

Abstract

The thermal melting curves of tRNAPhe and tRNALys from yeast lacking different numbers of nucleotides from the 3'-end were recorded. The removal of the first nucleotide had no effect on the melting profile. The stepwise removal of the following six nucleotides caused changes in the character of the derivative melting curves : they became rather broad and melting started at lower temperatures. These results indicate that the nucleotides at the 3'-end contribute to the stability of the molecular structure of tRNAs.

Single-molecule mechanical unfolding kinetics of unmodified Saccharomyces cerevisiae tRNAPhe: a hint to the tRNA chaperone-tRNA interaction mechanism

“…Unraveling the detailed mechanical folding and unfolding kinetics of native tRNA and tRNA intermediates may help understand the mechanism of these tRNAassociated biological functions. Interestingly, despite the great success achieved in tRNA folding research (13)(14)(15)(16)(17)(18)(19), the folding and unfolding kinetics of tRNAs, especially kinetics of tRNA intermediates remain largely (which was not certified by peer review) is the author/funder. All rights reserved.…”