Thermal model of capillary electrophoresis and a method for counteracting thermal band broadening

Gobie, William A.; Ivory, Cornelius F.

doi:10.1016/s0021-9673(01)90217-1

Cited by 145 publications

(126 citation statements)

References 10 publications

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“…The heat produced in the preparative DFGF device will increase proportionally with the applied electric field strength. Uncontrolled Joule heating leads to an autothermal effect [14] which would eventually boil the buffer [15], and cause arcing in the separation chamber. Heating will also denature proteins [7], robbing them of function.…”

Section: Preparative Dfgf Designmentioning

confidence: 99%

Assessing the scalability of dynamic field gradient focusing by linear modeling

Tracy

Ivory²

2008

J of Separation Science

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Dynamic field gradient focusing (DFGF) separates and concentrates proteins in native buffers, where proteins are most soluble, using a computer-controlled electric field gradient which lets the operator adjust the pace and resolution of the separation in real-time. The work in this paper assessed whether DFGF could be scaled up from microgram analytical-scale protein loads to milligram preparative-scale loads. Linear modeling of the electric potential, protein transport, and heat transfer simulated the performance of a preparative-scale DFGF instrument. The electric potential model showed where the electrodes should be placed to optimize the shape and strength of the electric field gradient. Results from the protein transport model suggested that in 10 min the device should separate 10 mg each of two proteins whose electrophoretic mobilities differ by 56. Proteins with electrophoretic mobilities differing by only 5% should separate in 3 h. The heat transfer model showed that the preparative DFGF design could dissipate 1 kW of Joule heat while keeping the separation chamber at 258C. Model results pointed to DFGF successfully scaling up by 10006 using the proposed instrument design.

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Section: Preparative Dfgf Designmentioning

confidence: 99%

Assessing the scalability of dynamic field gradient focusing by linear modeling

Tracy

Ivory²

2008

J of Separation Science

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“…The goal was to flatten the hydrodynamic velocity profile by opposing a laminar flow with a second laminar flow. Later, Gobie and Ivory [2] proposed to counter the thermally induced parabolic profile of the electrically driven migration with an opposing Poiseuille flow. Kok [3] suggested to apply a compensating pressure on the outlet end of the separation capillary to eliminate the effect of a band-broadening source introduced by the voltage decoupler into a separation capillary.…”

Section: Application Of Counterflowsmentioning

confidence: 99%

Potential of flow‐counterbalanced capillary electrophoresis for analytical and micropreparative separations

et al. 1999

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“…In this sense, the important influence of injection time on R s , N Fe(II) and N Ni(II) indicates that the dispersion effects due to the injected sample volume will be predominant. On the other hand, the dependence of the Ni(II) efficiency on the applied voltage can be explained if dominant diffusion effects are assumed at low voltages (higher migration time and diffusion) and Taylor dispersion due to the Joule effect [27,28] is assumed at high voltages. When the injection time is increased, these effects stop being predominant.…”

Section: Gold Processing Solutionsmentioning

confidence: 99%

Experimental designs and response surface modeling applied for the optimization of metal-cyanide complexes analysis by capillary electrophoresis

Martí¹,

Aguilar²,

Farrán³

1999

Electrophoresis

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The development of capillary zone electrophoresis (CZE) methods for the determination of metal cyanide complexes in real samples showed some problems, such as the low detection signal of Au (CN)2— and the low resolution between Ni(II) and Fe(II) cyanides in gold processing solutions, and the lack of separation of Pt(CN)42— and Pd(CN)42— in the leachates from automobile catalytic converters. To optimize some analytical parameters, the present study thus focused on the application of experimental designs and multiregression models. The following factors were examined by a two‐level factorial design: applied voltage, injection time, detection wavelength, buffer ion, ionic strength and buffer modifiers. For optimization of the CZE method, subsequent response‐surface experiments with the important factors were made with the two kinds of leaching solutions. Optimal analytical conditions were obtained in each case, giving good detection signals and resolution for the components of the studied leachates.

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Thermal model of capillary electrophoresis and a method for counteracting thermal band broadening

Cited by 145 publications

References 10 publications

Assessing the scalability of dynamic field gradient focusing by linear modeling

Assessing the scalability of dynamic field gradient focusing by linear modeling

Potential of flow‐counterbalanced capillary electrophoresis for analytical and micropreparative separations

Experimental designs and response surface modeling applied for the optimization of metal-cyanide complexes analysis by capillary electrophoresis

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