Thermal pretreatment of the solid fraction of manure: impact on the biogas reactor performance and microbial community

Mladenovska, Zuzana; Hartmann, Hinrich; Kvist, Torben; Sales‐Cruz, Mauricio; Gani, Rafiqul; Ahring, Birgitte Kiær

doi:10.2166/wst.2006.236

Cited by 69 publications

(30 citation statements)

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“…T-RFLP profiles (Mladenovska et al 2006) and DGGE band patterns (Bodelier et al 2005;Connaughton et al 2006) were found to reflect the change in the microbial population. In the present study, the microbial community dynamics in a CSTR during anaerobic co-digestion of cow manure and grass silage were investigated by 16S rRNA gene-based T-RFLP and DGGE analyses.…”

Section: Discussionmentioning

confidence: 97%

“…Both methods have several advantages over other fingerprinting methods, since they have high sample throughput, and enable the resolution of a complete target community. Very recently, DGGE and T-RFLP profiling have been used in exploring microbial community structure in the anaerobic digestion of industrial wastes (Connaughton et al 2006) and manure (Mladenovska et al 2006), and codigestion of food waste and biosolids (McMahon et al 2001). It is anticipated that knowledge on both the structure of microbial communities and the relations between microbial community structure and digester performance during anaerobic co-digestion of crops and manure would also be obtained by DGGE and T-RFLP profiling.…”

Section: Introductionmentioning

confidence: 99%

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Microbial community structure in anaerobic co-digestion of grass silage and cow manure in a laboratory continuously stirred tank reactor

et al. 2009

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The impacts of feeding ratio and loading rate on the microbial community during co-digestion of grass silage with cow manure in an anaerobic laboratory continuously stirred tank reactor were investigated by 16S rRNA gene-based fingerprints. The microbial community remained stable when the reactor was fed with cow manure alone and with up to 20% of grass silage in feedstock at an organic loading rate (OLR) of 2 kg VS m(-3) day(-1). Large changes in the bacterial community were observed when the loading ratio of grass was increased to 40%, while there was little change in the archaeal community. During the increase in OLR from 2 to 4 kg VS m(-3) day(-1) the bacterial community structure showed few differences, whereas Archaea was undetectable. Sequencing of the major DGGE bands indicated that the phylum Bacteriodetes predominated in the bacterial community. Two unclassified bacteria with high abundance survived throughout the operation of the reactor.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Microbial community structure in anaerobic co-digestion of grass silage and cow manure in a laboratory continuously stirred tank reactor

et al. 2009

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“…In a relatively recent investigation, laboratoryscale CSTRs were operated with manure, at 55°C, a HRT of 18 days, and an organic loading rate (OLR) of 2.5 g VS/l/day (Mladenovska et al 2005).…”

Section: The Effect Of Reactor Configurationmentioning

confidence: 99%

The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review

Demirel

Scherer

2008

Rev Environ Sci Biotechnol

987

497

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Among different conversion processes for biomass, biological anaerobic digestion is one of the most economic ways to produce biogas from various biomass substrates. In addition to hydrolysis of polymeric substances, the activity and performance of the methanogenic bacteria is of paramount importance during methanogenesis. The aim of this paper is primarily to review the recent literature about the occurrence of both acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of particulate biomass to methane (not wastewater treatment), while this review does not cover the activity of the acetate oxidizing bacteria. Both acetotrophic and hydrogenotrophic methanogens are essential for the last step of methanogenesis, but the reports about their roles during this phase of the process are very limited. Despite, some conclusions can still be drawn. At low concentrations of acetate, normally filamentous Methanosaeta species dominate, e.g., often observed in sewage sludge. Apparently, high concentrations of toxic ionic agents, like ammonia, hydrogen sulfide (H 2 S) and volatile fatty acids (VFA), inhibit preferably Methanosaetaceae and especially allow the growth of Methanosarcina species consisting of irregular cell clumps, e.g., in cattle manure. Thermophilic conditions can favour rod like or coccoid hydrogenotrophic methanogens. Thermophilic Methanosarcina species were also observed, but not thermophilic Methanosaetae. Other environmental factors could favour hydrogentrophic bacteria, e.g., short or low retention times in a biomass reactor. However, no general rules regarding process parameters could be derivated at the moment, which favours hydrogenotrophic methanogens. Presumably, it depends only on the hydrogen concentration, which is generally not mentioned in the literature.

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“…[6]. Thermal pretreatment of the solid fraction of manure was reported to improve the yield by 9%-24% [7]. Extrusion applied on straw and the solid fraction of manure resulted in 70% and 27% increases, respectively [8].…”

Section: Introductionmentioning

confidence: 99%

Improving Anaerobic Digestion of Wheat Straw by Plasma-Assisted Pretreatment

Heiske

Schultz-Jensen

Leipold

et al. 2013

Journal of Atomic and Molecular Physics

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Plasma-assisted pretreatment (PAP) of lignocellulosic biomass has been shown to be an efficient method to decompose lignin and consequently facilitate microbial access to cellulose and hemicellulose. In the present study, PAP was tested for its suitability to enhance bioconversion of wheat straw to methane. In thermophilic batch experiments, methane yields of up to 366 mL/g volatile solids (VSs) were achieved, accounting for a yield increase of 45%. Common lignin-derived inhibitors like 5-hydroxymethylfurfural (5-HMF) and furfural were not detected after PAP, but toxicity test resulted in lower methane yields at higher substrate concentrations, indicating the presence of other unidentified inhibitors. However, in a continuous lab-scale biogas reactor experiment, stable codigestion of cattle manure with 20% PAP wheat straw was demonstrated, while no signs of adverse effects on the anaerobic digestion process were observed. After the introduction of the pretreated wheat straw to the reactor, volatile fatty acid concentrations remained low and stable, while gas production increased. In co-digestion, the PAP wheat straw was converted at an average yield of 343 mL CH 4 /gVS.

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Thermal pretreatment of the solid fraction of manure: impact on the biogas reactor performance and microbial community

Cited by 69 publications

References 0 publications

Microbial community structure in anaerobic co-digestion of grass silage and cow manure in a laboratory continuously stirred tank reactor

Microbial community structure in anaerobic co-digestion of grass silage and cow manure in a laboratory continuously stirred tank reactor

The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review

Improving Anaerobic Digestion of Wheat Straw by Plasma-Assisted Pretreatment

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