Thermal Regulation of FMLP Receptors on Human Neutrophils

Abstract

Polymorphonuclear leukocytes (PMNs) from subjects diagnosed as having juvenile periodontitis (JP) have been categorized on the basis of their chemotactic (CTX) response to f-met-leu-phe (FMLP) when assayed concurrently with PMNs from periodontally healthy subjects (HP). When PMNs from JP groups demonstrating depressed CTX were assayed for lysosomal enzyme secretion (LES) in response to FMLP, there were no significant differences with respect to rate or amount. Significant differences were observed between HP and chemotactically depressed JP cells when assessed for FMLP receptor ligand binding at 23 degrees C, but not at 4 degrees C. Receptor differences observed at 23 degrees C in HP cells included an increase in amount of total binding, number of receptors, and available displaceable binding sites, compared with the chemotactically depressed JP PMNs, whereas the receptor affinities were similar. These data suggest that differences in FMLP receptor density in JP PMN that are chemotactically depressed may be related to processes that modulate receptor mobility and/or expression.

Granulocytes from chronic myeloid leukemia (CML) patients show differential response to different chemoattractants

“…Chemotaxis dysfunction in humans with juvenile periodontitis is also accompanied by diminished receptor-mediated binding of chemoattractant. The neutrophils exhibit fewer than normal cell surface receptors for fMLP [38].…”

Altered chemotactic behavior of crevicular PMNs in different forms of periodontitis

“…Bacterial invasion in the gingival crevice initiates the elaboration of a variety of substances which have the ability to attract PMN to the site of inflammation (chemotaxis). Chemotaxis disorders of peripheral blood PMN have been described in some forms of early onset periodontitis (EOP) including localised early onset periodontitis (LEOP), generalised early onset periodontitis (GEOP), and pre-pubertal periodontitis (Page & Schroeder 1982, Van Dyke et al 1981, Van Dyke et al 1985, 1987, Coles et al 1989, Daniel et al 1993. Deficiencies of neutrophil function often result in increased susceptibility to periodontitis.…”

“…Deficiencies of neutrophil function often result in increased susceptibility to periodontitis. Chemotaxis disorders of peripheral blood PMN have been described in some forms of early onset periodontitis (EOP) including localised early onset periodontitis (LEOP), generalised early onset periodontitis (GEOP), and pre-pubertal periodontitis (Page & Schroeder 1982, Van Dyke et al 1981, Van Dyke et al 1985, 1987, Coles et al 1989, Daniel et al 1993). These authors reported mainly about a reduced chemotactic function in LEOP patients, but others have discussed an enhanced chemotactic response of peripheral blood PMN in these periodontitis forms (Kinane et al 1989, Repo et al 1990).…”