2013
DOI: 10.1016/j.vascn.2013.02.003
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Thermal sensitivity as a measure of spontaneous morphine withdrawal in mice

Abstract: Introduction Opioid withdrawal syndrome is a critical component of opioid abuse and consists of a wide array of symptoms including increases in pain sensitivity (hyperalgesia). A reliable preclinical model of hyperalgesia during opioid withdrawal is needed to evaluate possible interventions to alleviate withdrawal. The following study describes a method for assessing increases in thermal sensitivity on the hotplate in a mouse model of spontaneous morphine withdrawal. Methods C57BL/6J mice received 5.5 days o… Show more

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Cited by 16 publications
(4 citation statements)
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“…Analgesia-like responses in were measured as previously described 55 using a hotplate analgesia meter with dimensions of 29.2 × 26.7 cm with mice restricted to a cylinder 8.9 cm in diameter and 15.2 cm high (IITC Life Sciences, Woodland Hills, California). Response was measured by recording the latency to lick, flutter, or splay hind paw(s), or an attempt to jump out of the apparatus at 55 °C, with a maximum cut-off time of 30 s. Once a response was observed or the cut-off time had elapsed, the subject was immediately removed from the hotplate and placed back in its home cage.…”
Section: Methodsmentioning
confidence: 99%
“…Analgesia-like responses in were measured as previously described 55 using a hotplate analgesia meter with dimensions of 29.2 × 26.7 cm with mice restricted to a cylinder 8.9 cm in diameter and 15.2 cm high (IITC Life Sciences, Woodland Hills, California). Response was measured by recording the latency to lick, flutter, or splay hind paw(s), or an attempt to jump out of the apparatus at 55 °C, with a maximum cut-off time of 30 s. Once a response was observed or the cut-off time had elapsed, the subject was immediately removed from the hotplate and placed back in its home cage.…”
Section: Methodsmentioning
confidence: 99%
“…Response was measured by recording the latency to lick, flutter, or splay hindpaw(s) or an attempt to jump out of the apparatus at 55°C, with a maximum cutoff time of 30 seconds. Once a response was observed or the cutoff time had elapsed, the subject was immediately removed from the hotplate and placed back in its home cage (Balter and Dykstra, 2013). The animals were acclimated to the hotplate, while cool, and a baseline analgesic response time was acquired several hours before drug treatment and testing.…”
Section: Hotplate Assaymentioning
confidence: 99%
“…Water and food were available ad libitum. The C57BL/6 J strain was selected because of its higher sensitivity to behavioral tests and lower inter-animal variability compared with other inbred strains [41,42]. All studies were conducted ethically in accordance with the Research Ethical Committee of UNIFESP (protocol #0675/09, June 19, 2010).…”
Section: Animalsmentioning
confidence: 99%