2002
DOI: 10.1006/biol.2002.0322
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Thermal Stability: A Means to Assure Tertiary Structure in Therapeutic Proteins

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Cited by 20 publications
(13 citation statements)
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“…Because the structural transition to intermolecular b-sheets occurs regardless of the initial secondary structure composition of the native protein, these bands can be used to monitor denaturation and aggregation reactions in both aqueous and solid state (4). Cauchy et al (40) plotted the fraction of denatured recombinant human growth hormone vs. temperature to obtain the thermal transition temperature as midpoint of the thermal denaturation curve and to calculate the free energy (DG u ) and enthalpy (DH u ) of protein unfolding. The protein unfolded fraction (f u ) was calculated by use of the peak height of an amide I frequency at 1620 cm j1 , which can be related to intermolecular b-sheet formation, and is based on an equation used by Pace et al (11) for the analysis of protein unfolding curves.…”
Section: Determination Of T M (Ftir) Of the Four Proteinsmentioning
confidence: 99%
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“…Because the structural transition to intermolecular b-sheets occurs regardless of the initial secondary structure composition of the native protein, these bands can be used to monitor denaturation and aggregation reactions in both aqueous and solid state (4). Cauchy et al (40) plotted the fraction of denatured recombinant human growth hormone vs. temperature to obtain the thermal transition temperature as midpoint of the thermal denaturation curve and to calculate the free energy (DG u ) and enthalpy (DH u ) of protein unfolding. The protein unfolded fraction (f u ) was calculated by use of the peak height of an amide I frequency at 1620 cm j1 , which can be related to intermolecular b-sheet formation, and is based on an equation used by Pace et al (11) for the analysis of protein unfolding curves.…”
Section: Determination Of T M (Ftir) Of the Four Proteinsmentioning
confidence: 99%
“…In contrast to the spectroscopic methods, one of the most important features of nDSC data is that the analysis does not require any assumption about the presence or absence of stable intermediates in the unfolding process. Furthermore, multistate transitions can be either detected directly or separated by a deconvolution algorithm (40,42). Nevertheless, the separate transition of the a-helix structure of b-LG occurring at a lower temperature in FTIR analysis compared to the changes in both the inter-and intramolecular b-sheet structures was not apparent in the calorimetric scans.…”
Section: Determination Of T M (Ndsc) Of the Four Proteinsmentioning
confidence: 99%
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